Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA.

abstract：:The ubiquitination cascade has a key role in the assembly of repair and signaling proteins at sites of double-strand DNA breaks. The E3 ubiquitin ligase RING finger protein 8 (RNF8) triggers the initial ubiquitination at double-strand DNA breaks, whereas sustained ubiquitination requires the downstream E3 ligase RING ...

journal_title：Nature structural & molecular biology

authors： Feng L,Chen J

更新日期：2012-01-22 00:00:00

abstract：:We have isolated a soluble cytochrome from Shewanella oneidensis that contains eight covalently attached heme groups and determined its crystal structure. One of these hemes exhibits novel ligation of the iron atom by the epsilon-amino group of a lysine residue, despite its attachment via a typical CXXCH motif. This h...

journal_title：Nature structural & molecular biology

authors： Mowat CG,Rothery E,Miles CS,McIver L,Doherty MK,Drewette K,Taylor P,Walkinshaw MD,Chapman SK,Reid GA

更新日期：2004-10-01 00:00:00

abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

journal_title：Nature structural & molecular biology

authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

更新日期：2014-05-01 00:00:00

abstract：:In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the ...

journal_title：Nature structural & molecular biology

authors： Yoshizawa S,Rasubala L,Ose T,Kohda D,Fourmy D,Maenaka K

更新日期：2005-02-01 00:00:00

abstract：:Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by degradation of nascent peptides. However, endogenous RQC-inducing sequences and the mechanism underlying the...

journal_title：Nature structural & molecular biology

authors： Matsuo Y,Tesina P,Nakajima S,Mizuno M,Endo A,Buschauer R,Cheng J,Shounai O,Ikeuchi K,Saeki Y,Becker T,Beckmann R,Inada T

更新日期：2020-04-01 00:00:00

abstract：:To locate key RNA features in the structure of the spliceosome by EM, we fused a sequence-specific RNA binding protein to a protein with a distinct donut-shaped structure. We used this fusion to label spliceosomes assembled on a pre-mRNA that contained the target sequence in the exons. The label is clearly visible in ...

journal_title：Nature structural & molecular biology

authors： Alcid EA,Jurica MS

更新日期：2008-02-01 00:00:00

abstract：:We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibo...

journal_title：Nature structural & molecular biology

authors： Egelhofer TA,Minoda A,Klugman S,Lee K,Kolasinska-Zwierz P,Alekseyenko AA,Cheung MS,Day DS,Gadel S,Gorchakov AA,Gu T,Kharchenko PV,Kuan S,Latorre I,Linder-Basso D,Luu Y,Ngo Q,Perry M,Rechtsteiner A,Riddle NC,Schwar

更新日期：2011-01-01 00:00:00

abstract：:To determine which genomic features promote homologous recombination, we created a genome-wide map of gene targeting sites. We used an adeno-associated virus vector to target identical loci introduced as transcriptionally active retroviral vectors. A comparison of ~2,000 targeted and untargeted sites showed that targe...

journal_title：Nature structural & molecular biology

authors： Deyle DR,Hansen RS,Cornea AM,Li LB,Burt AA,Alexander IE,Sandstrom RS,Stamatoyannopoulos JA,Wei CL,Russell DW

更新日期：2014-11-01 00:00:00

abstract：:Although the two B-family human DNA polymerases, pol δ and pol ε, are responsible for the bulk of nuclear genome replication, at least 14 additional polymerases have roles in nuclear DNA repair and replication. In this issue, newly reported crystal structures of two specialized A-family polymerases, pol δ and pol ε, e...

journal_title：Nature structural & molecular biology

authors： Beard WA,Wilson SH

更新日期：2015-04-01 00:00:00

abstract：:In budding yeast, DNA damage in G1 activates a Rad9-dependent checkpoint that targets the cyclin-dependent kinase (CDK) Cdc28 to delay G1 exit. After a transient arrest, cells may enter S phase before completing DNA repair. We used genetic analysis to identify the stress-responsive CDK Pho85, the cyclin Pho80 and the ...

journal_title：Nature structural & molecular biology

authors： Wysocki R,Javaheri A,Kristjansdottir K,Sha F,Kron SJ

更新日期：2006-10-01 00:00:00

abstract：:The histone variant macroH2A1 regulates gene expression important for differentiation, stem-cell reprogramming and tumor suppression. Here, we demonstrate that in primary human cells, macroH2A1 participates in two physically and functionally distinct types of chromatin marked by either H3K27me3 or nine histone acetyla...

journal_title：Nature structural & molecular biology

authors： Chen H,Ruiz PD,Novikov L,Casill AD,Park JW,Gamble MJ

更新日期：2014-11-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:Although R loops can occur at different genomic locations, the factors that determine their formation and frequency remain unclear. Emerging evidence indicates that DNA breaks stimulate DNA-RNA hybrid formation. Here, we discuss the possibility that formation of hybrids may be an inevitable risk of DNA breaks that occ...

journal_title：Nature structural & molecular biology

authors： Aguilera A,Gómez-González B

更新日期：2017-05-04 00:00:00

abstract：:Otopetrins (Otop1-Otop3) comprise one of two known eukaryotic proton-selective channel families. Otop1 is required for otoconia formation and a candidate mammalian sour taste receptor. Here we report cryo-EM structures of zebrafish Otop1 and chicken Otop3 in lipid nanodiscs. The structures reveal a dimeric architectur...

journal_title：Nature structural & molecular biology

authors： Saotome K,Teng B,Tsui CCA,Lee WH,Tu YH,Kaplan JP,Sansom MSP,Liman ER,Ward AB

更新日期：2019-06-01 00:00:00

abstract：:The ≥ 10³⁰ bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanis...

journal_title：Nature structural & molecular biology

authors： Blower TR,Pei XY,Short FL,Fineran PC,Humphreys DP,Luisi BF,Salmond GP

更新日期：2011-02-01 00:00:00

abstract：:Desensitization is a universal feature of ligand-gated ion channels. Using the crystal structure of the GluR2 L483Y mutant channel as a guide, we attempted to build non-desensitizing kainate-subtype glutamate receptors. Success was achieved for GluR5, GluR6 and GluR7 with intermolecular disulfide cross-links but not b...

journal_title：Nature structural & molecular biology

authors： Weston MC,Schuck P,Ghosal A,Rosenmund C,Mayer ML

更新日期：2006-12-01 00:00:00

abstract：:The type III secretion system (T3SS) is a macromolecular 'injectisome' that allows bacterial pathogens to transport virulence proteins into the eukaryotic host cell. This macromolecular complex is composed of connected ring-like structures that span both bacterial membranes. The crystal structures of the periplasmic d...

journal_title：Nature structural & molecular biology

authors： Spreter T,Yip CK,Sanowar S,André I,Kimbrough TG,Vuckovic M,Pfuetzner RA,Deng W,Yu AC,Finlay BB,Baker D,Miller SI,Strynadka NC

更新日期：2009-05-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression, including type I interferon production, by promoting host mRNA degradation and inhibiting host translation, in infected cells. We present evidence that nsp1 uses a novel, two-pronged strategy to inhibit host translation and gen...

journal_title：Nature structural & molecular biology

authors： Kamitani W,Huang C,Narayanan K,Lokugamage KG,Makino S

更新日期：2009-11-01 00:00:00

abstract：:The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry ...

journal_title：Nature structural & molecular biology

authors： Park YJ,Walls AC,Wang Z,Sauer MM,Li W,Tortorici MA,Bosch BJ,DiMaio F,Veesler D

更新日期：2019-12-01 00:00:00

abstract：:Neuronal communication is mediated by Ca(2+)-triggered fusion of transmitter-filled synaptic vesicles with the presynaptic plasma membrane. Synaptotagmin I functions as a Ca(2+) sensor that regulates exocytosis, whereas soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins in th...

journal_title：Nature structural & molecular biology

authors： Chicka MC,Hui E,Liu H,Chapman ER

更新日期：2008-08-01 00:00:00

abstract：:During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation comp...

journal_title：Nature structural & molecular biology

authors： Bernecky C,Plitzko JM,Cramer P

更新日期：2017-10-01 00:00:00

abstract：:Although t-loops protect telomeres, they are at risk of cleavage by Holliday junction (HJ) resolvases if branch migration converts the three-way t-loop junction into four-way HJs. T-loop cleavage is repressed by the TRF2 basic domain, which binds three- and four-way junctions and protects HJs in vitro. By replacing th...

journal_title：Nature structural & molecular biology

authors： Schmutz I,Timashev L,Xie W,Patel DJ,de Lange T

更新日期：2017-09-01 00:00:00

abstract：:Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How these events are coordinated with the cell cycle is poorly understood, particularly in mammalian cells. We report here, based on newly developed techniques for visualizing NPC formation,...

journal_title：Nature structural & molecular biology

authors： Maeshima K,Iino H,Hihara S,Funakoshi T,Watanabe A,Nishimura M,Nakatomi R,Yahata K,Imamoto F,Hashikawa T,Yokota H,Imamoto N

更新日期：2010-09-01 00:00:00

abstract：:Human telomeres are associated with ATM and the protein complex consisting of MRE11, RAD50 and NBS1 (MRN), which are central to maintaining genomic stability. Here we show that when targeted to telomeres, wild-type RAD50 downregulates telomeric association of TRF1, a negative regulator of telomere maintenance. TRF1 bi...

journal_title：Nature structural & molecular biology

authors： Wu Y,Xiao S,Zhu XD

更新日期：2007-09-01 00:00:00

abstract：:RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the bind...

journal_title：Nature structural & molecular biology

authors： Kassube SA,Jinek M,Fang J,Tsutakawa S,Nogales E

更新日期：2013-07-01 00:00:00

abstract：:The typical function of kinesins is to transport cargo along microtubules. Binding of ATP to microtubule-attached motile kinesins leads to cargo displacement. To better understand the nature of the conformational changes that lead to the power stroke that moves a kinesin's load along a microtubule, we determined the X...

journal_title：Nature structural & molecular biology

authors： Gigant B,Wang W,Dreier B,Jiang Q,Pecqueur L,Plückthun A,Wang C,Knossow M

更新日期：2013-08-01 00:00:00

abstract：:Histones have two structurally and functionally distinct domains: globular domains forming the nucleosomal core around which DNA is wrapped and unstructured tails protruding from the nucleosomal core. Whereas post-translational modifications (PTMs) in histone tails are well studied, much less is currently known about ...

journal_title：Nature structural & molecular biology

authors： Tropberger P,Schneider R

更新日期：2013-06-01 00:00:00

abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

journal_title：Nature structural & molecular biology

authors： Vincent JA,Kwong TJ,Tsukiyama T

更新日期：2008-05-01 00:00:00

abstract：:The extracellular domain of the metabotropic glutamate receptor 1alpha (mGluR1alpha) forms a dimer and the ligand, glutamate, induces a structural rearrangement in this domain. However, the conformational change in the cytoplasmic domain, which is critical for mGluR1alpha's interaction with G proteins, remains unclear...

journal_title：Nature structural & molecular biology

authors： Tateyama M,Abe H,Nakata H,Saito O,Kubo Y

更新日期：2004-07-01 00:00:00

abstract：:We show that convergent transcription induces transcriptional gene silencing (TGS) in trans for both fission yeast and mammalian cells. This method has advantages over existing strategies to induce gene silencing. Previous studies in fission yeast have characterized TGS as a cis-specific process involving RNA interfer...

journal_title：Nature structural & molecular biology

authors： Gullerova M,Proudfoot NJ

更新日期：2012-11-01 00:00:00