Abstract:
:Structural maintenance of chromosome (SMC) complexes are central regulators of chromosome architecture that are essential in all domains of life. For decades, the structural biology field has been debating how these conserved protein complexes use their intricate ring-like structures to structurally organize DNA. Here, we review the contributions of single-molecule biophysical approaches to resolving the molecular mechanism of SMC protein function.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Eeftens J,Dekker Cdoi
10.1038/nsmb.3507subject
Has Abstractpub_date
2017-12-07 00:00:00pages
1012-1020issue
12eissn
1545-9993issn
1545-9985pii
nsmb.3507journal_volume
24pub_type
杂志文章,评审abstract::In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the ...
journal_title:Nature structural & molecular biology
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doi:10.1038/nsmb890
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abstract::Hsp70s use ATP hydrolysis to disrupt protein-protein associations and to move macromolecules. One example is the Hsc70- mediated disassembly of the clathrin coats that form on vesicles during endocytosis. Here, we exploited the exceptional features of these coats to test three models-Brownian ratchet, power-stroke and...
journal_title:Nature structural & molecular biology
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journal_title:Nature structural & molecular biology
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb1063
更新日期:2006-04-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 历史文章,杂志文章,评审
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb.3302
更新日期:2016-11-01 00:00:00
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journal_title:Nature structural & molecular biology
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb1110
更新日期:2006-07-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/s41594-018-0171-0
更新日期:2019-01-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb1352
更新日期:2008-01-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb.2345
更新日期:2012-08-01 00:00:00
abstract::Microtubules are regulated by post-translational modifications of tubulin. The ligation and cleavage of the carboxy-terminal tyrosine of α-tubulin impact microtubule functions during mitosis, cardiomyocyte contraction and neuronal processes. Tubulin tyrosination and detyrosination are mediated by tubulin tyrosine liga...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/s41594-019-0242-x
更新日期:2019-07-01 00:00:00
abstract::During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/s41594-017-0002-8
更新日期:2018-01-01 00:00:00
abstract::The Rbfox family of splicing factors regulate alternative splicing during animal development and in disease, impacting thousands of exons in the maturing brain, heart and muscle. Rbfox proteins have long been known to bind to the RNA sequence GCAUG with high affinity and specificity, but just half of Rbfox binding sit...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/s41594-020-0475-8
更新日期:2020-10-01 00:00:00
abstract::Histone H3 Lys4 (H3K4) methylation is a prevalent mark associated with transcription activation. A common feature of several H3K4 methyltransferase complexes is the presence of three structural components (RbBP5, Ash2L and WDR5) and a catalytic subunit containing a SET domain. Here we report the first biochemical reco...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb.2332
更新日期:2012-06-24 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb.2565
更新日期:2013-05-01 00:00:00
abstract::How do the key features of protein folding, elucidated from studies on native, isolated proteins, manifest in cotranslational folding on the ribosome? Using a well-characterized family of homologous α-helical proteins with a range of biophysical properties, we show that spectrin domains can fold vectorially on the rib...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb.3355
更新日期:2017-03-01 00:00:00
abstract::The GTPase dynamin is essential for numerous vesiculation events including clathrin-mediated endocytosis. Upon GTP hydrolysis, dynamin constricts a lipid bilayer. Previously, a three-dimensional structure of mutant dynamin in the constricted state was determined by helical reconstruction methods. We solved the noncons...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb762
更新日期:2004-06-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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更新日期:2012-01-08 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb770
更新日期:2004-07-01 00:00:00
abstract::The PHD finger protein 1 (PHF1) is essential in epigenetic regulation and genome maintenance. Here we show that the Tudor domain of human PHF1 binds to histone H3 trimethylated at Lys36 (H3K36me3). We report a 1.9-Å resolution crystal structure of the Tudor domain in complex with H3K36me3 and describe the molecular me...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
abstract::The RNA-induced transcriptional silencing (RITS) complex, containing Ago1, Chp1, Tas3 and centromeric small interfering RNAs (siRNAs), is required for heterochromatic gene silencing at centromeres. Here, we identify a second fission yeast Argonaute complex (Argonaute siRNA chaperone, ARC), which contains, in addition ...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb1211
更新日期:2007-03-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature structural & molecular biology
pub_type: 已发布勘误
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更新日期:2020-09-01 00:00:00
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journal_title:Nature structural & molecular biology
pub_type: 杂志文章
doi:10.1038/nsmb.1378
更新日期:2008-02-01 00:00:00
abstract::Coupling between transcription and RNA processing is a key gene regulatory mechanism. Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation of the precursor mRNA (pre-mRNA) splicing factors hnRNP A1, U2AF65 and U1 and U5 snRNPs on the intron-containing human FOS gene. These factors w...
journal_title:Nature structural & molecular biology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00