Structural basis for the coevolution of a viral RNA-protein complex.

abstract：:Bacteriophages express proteins that inactivate the CRISPR-Cas bacterial immune system. Here we report the crystal structure of the anti-CRISPR protein AcrF3 in complex with Pseudomonas aeruginosa Cas3 (PaCas3). AcrF3 forms a homodimer that locks PaCas3 in an ADP-bound form, blocks the entrance of the DNA-binding tunn...

journal_title：Nature structural & molecular biology

authors： Wang X,Yao D,Xu JG,Li AR,Xu J,Fu P,Zhou Y,Zhu Y

更新日期：2016-09-01 00:00:00

abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

journal_title：Nature structural & molecular biology

authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

更新日期：2012-06-24 00:00:00

abstract：:Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using alpha-amanitin-resistant pol II CTD mutants and fibrone...

journal_title：Nature structural & molecular biology

authors： de la Mata M,Kornblihtt AR

更新日期：2006-11-01 00:00:00

abstract：:Beta-2 microglobulin (beta2m) is a globular protein that self-associates into fibrillar amyloid deposits in patients undergoing hemodialysis therapy. Formation of these beta-sheet-rich assemblies is a fundamental property of polypeptides that can be triggered by diverse conditions. For beta2m, oligomerization into pre...

journal_title：Nature structural & molecular biology

authors： Calabrese MF,Eakin CM,Wang JM,Miranker AD

更新日期：2008-09-01 00:00:00

abstract：:Although DNA replication is a fundamental aspect of biology, it is not known what determines where DNA replication starts and stops in the human genome. We directly identified and quantitatively compared sites of replication initiation and termination in untransformed human cells. We found that replication preferentia...

journal_title：Nature structural & molecular biology

authors： Chen YH,Keegan S,Kahli M,Tonzi P,Fenyö D,Huang TT,Smith DJ

更新日期：2019-01-01 00:00:00

abstract：:The ability of barrier-to-autointegration factor (BAF) to bind and bridge DNA in a sequence-independent manner is crucial for its role in retroviral integration and a variety of cellular processes. To better understand this behavior, we solved the crystal structure of BAF bound to DNA. The structure reveals that BAF b...

journal_title：Nature structural & molecular biology

authors： Bradley CM,Ronning DR,Ghirlando R,Craigie R,Dyda F

更新日期：2005-10-01 00:00:00

abstract：:Binding of the gp120 envelope (Env) glycoprotein to the CD4 receptor is the first step in the HIV-1 infectious cycle. Although the CD4-binding site has been extensively characterized, the initial receptor interaction has been difficult to study because of major CD4-induced structural rearrangements. Here we used cryog...

journal_title：Nature structural & molecular biology

authors： Liu Q,Acharya P,Dolan MA,Zhang P,Guzzo C,Lu J,Kwon A,Gururani D,Miao H,Bylund T,Chuang GY,Druz A,Zhou T,Rice WJ,Wigge C,Carragher B,Potter CS,Kwong PD,Lusso P

更新日期：2017-04-01 00:00:00

abstract：:Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by c...

journal_title：Nature structural & molecular biology

authors： Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

更新日期：2006-08-01 00:00:00

abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

journal_title：Nature structural & molecular biology

authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

更新日期：2014-05-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Mahalingan KK,Keith Keenan E,Strickland M,Li Y,Liu Y,Ball HL,Tanner ME,Tjandra N,Roll-Mecak A

更新日期：2020-09-01 00:00:00

abstract：:The monoclonal antibody 13H11 shares part of its epitope in the HIV-1 gp41 membrane-proximal external region (MPER) with the rare, broadly neutralizing human antibody 2F5. Although 13H11 partially cross-blocked 2F5 binding, 13H11 is non-neutralizing and does not block 2F5 neutralization. We show that unlike 2F5, 13H11...

journal_title：Nature structural & molecular biology

authors： Nicely NI,Dennison SM,Spicer L,Scearce RM,Kelsoe G,Ueda Y,Chen H,Liao HX,Alam SM,Haynes BF

更新日期：2010-12-01 00:00:00

abstract：:The Saccharomyces cerevisiae proteasome comprises a 19-subunit regulatory particle and a 28-subunit core particle. To be degraded, substrates must cross the core particle-regulatory particle interface, a site for complex conformational changes and regulatory events. This interface includes two aligned heteromeric ring...

journal_title：Nature structural & molecular biology

authors： Tian G,Park S,Lee MJ,Huck B,McAllister F,Hill CP,Gygi SP,Finley D

更新日期：2011-10-30 00:00:00

abstract：:Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-ind...

journal_title：Nature structural & molecular biology

authors： Bühler M,Steiner S,Mohn F,Paillusson A,Mühlemann O

更新日期：2006-05-01 00:00:00

abstract：:We have isolated a soluble cytochrome from Shewanella oneidensis that contains eight covalently attached heme groups and determined its crystal structure. One of these hemes exhibits novel ligation of the iron atom by the epsilon-amino group of a lysine residue, despite its attachment via a typical CXXCH motif. This h...

journal_title：Nature structural & molecular biology

authors： Mowat CG,Rothery E,Miles CS,McIver L,Doherty MK,Drewette K,Taylor P,Walkinshaw MD,Chapman SK,Reid GA

更新日期：2004-10-01 00:00:00

abstract：:The association of Zika virus (ZIKV) infections with microcephaly has resulted in an ongoing public-health emergency. Here we report the crystal structure of a C-terminal fragment of ZIKV nonstructural protein 1 (NS1), a major host-interaction molecule that functions in flaviviral replication, pathogenesis and immune ...

journal_title：Nature structural & molecular biology

authors： Song H,Qi J,Haywood J,Shi Y,Gao GF

更新日期：2016-05-01 00:00:00

abstract：:When targeting promoter regions, small interfering RNAs (siRNAs) trigger a previously proposed pathway known as transcriptional gene silencing by promoting heterochromatin formation. Here we show that siRNAs targeting intronic or exonic sequences close to an alternative exon regulate the splicing of that exon. The eff...

journal_title：Nature structural & molecular biology

authors： Alló M,Buggiano V,Fededa JP,Petrillo E,Schor I,de la Mata M,Agirre E,Plass M,Eyras E,Elela SA,Klinck R,Chabot B,Kornblihtt AR

更新日期：2009-07-01 00:00:00

abstract：: ...

journal_title：Nature structural & molecular biology

authors： Statsyuk AV

更新日期：2018-06-01 00:00:00

abstract：:In this article, the Ponceau staining presented in Fig. 1b (right, bottom) does not follow best practices for figure preparation since itinadvertently included duplications from the Ponceau staining presented in Supplementary Fig. 1b (for which the same preparation ofnucleosomes from HeLa cells had been used). A new F...

journal_title：Nature structural & molecular biology

authors： Daujat S,Weiss T,Mohn F,Lange UC,Ziegler-Birling C,Zeissler U,Lappe M,Schübeler D,Torres-Padilla ME,Schneider R

更新日期：2018-08-01 00:00:00

abstract：:Thousands of long noncoding RNAs (lncRNAs) have been discovered, but their functional characterization has been slowed by a limited set of research tools. Here we review emerging RNA-centric methods to interrogate the intrinsic structure of lncRNAs as well as their genomic localization and biochemical partners. Unders...

journal_title：Nature structural & molecular biology

authors： Chu C,Spitale RC,Chang HY

更新日期：2015-01-01 00:00:00

abstract：:Heterochromatin causes epigenetic repression that can be transmitted through multiple cell divisions. However, the mechanisms underlying silencing and stability of heterochromatin are not fully understood. We show that heterochromatin differs from euchromatin in histone turnover and identify histone deacetylase (HDAC)...

journal_title：Nature structural & molecular biology

authors： Aygün O,Mehta S,Grewal SI

更新日期：2013-05-01 00:00:00

abstract：:The proteasome is the main ATP-dependent protease in eukaryotic cells and controls the concentration of many regulatory proteins in the cytosol and nucleus. Proteins are targeted to the proteasome by the covalent attachment of polyubiquitin chains. The ubiquitin modification serves as the proteasome recognition elemen...

journal_title：Nature structural & molecular biology

authors： Prakash S,Tian L,Ratliff KS,Lehotzky RE,Matouschek A

更新日期：2004-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Nicoll CR,Bailleul G,Fiorentini F,Mascotti ML,Fraaije MW,Mattevi A

更新日期：2020-02-01 00:00:00

abstract：:Primary microRNA cleavage by the Drosha-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the au...

journal_title：Nature structural & molecular biology

authors： Barad O,Mann M,Chapnik E,Shenoy A,Blelloch R,Barkai N,Hornstein E

更新日期：2012-05-13 00:00:00

abstract：:Processing of microRNAs (miRNAs) from their precursors to their biologically active mature forms is regulated during development and cancer. We show that mouse pri- or pre-miR-151 can bind to and compete with mature miR-151-5p and miR-151-3p for binding sites contained within the complementary regions of the E2f6 mRNA...

journal_title：Nature structural & molecular biology

authors： Roy-Chaudhuri B,Valdmanis PN,Zhang Y,Wang Q,Luo QJ,Kay MA

更新日期：2014-09-01 00:00:00

abstract：:Bacteriophage T4 and related viruses have a contractile tail that serves as an efficient mechanical device for infecting bacteria. A three-dimensional cryo-EM reconstruction of the mature T4 tail assembly at 15-A resolution shows the hexagonal dome-shaped baseplate, the extended contractile sheath, the long tail fiber...

journal_title：Nature structural & molecular biology

authors： Kostyuchenko VA,Chipman PR,Leiman PG,Arisaka F,Mesyanzhinov VV,Rossmann MG

更新日期：2005-09-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) play critical roles during tumorigenesis by functioning as scaffolds that regulate protein-protein, protein-DNA or protein-RNA interactions. Using a clinically guided genetic screening approach, we identified lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1), which is overexpre...

journal_title：Nature structural & molecular biology

authors： Zhang Y,He Q,Hu Z,Feng Y,Fan L,Tang Z,Yuan J,Shan W,Li C,Hu X,Tanyi JL,Fan Y,Huang Q,Montone K,Dang CV,Zhang L

更新日期：2016-06-01 00:00:00

abstract：:To our knowledge, no structural study to date has characterized, in an intact receptor, the coupling of conformational change in extracellular domains through a single-pass transmembrane domain to conformational change in cytoplasmic domains. Here we examine such coupling, and its unexpected complexity, using nearly f...

journal_title：Nature structural & molecular biology

authors： Mi LZ,Lu C,Li Z,Nishida N,Walz T,Springer TA

更新日期：2011-08-07 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression, including type I interferon production, by promoting host mRNA degradation and inhibiting host translation, in infected cells. We present evidence that nsp1 uses a novel, two-pronged strategy to inhibit host translation and gen...

journal_title：Nature structural & molecular biology

authors： Kamitani W,Huang C,Narayanan K,Lokugamage KG,Makino S

更新日期：2009-11-01 00:00:00

abstract：:An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from h...

journal_title：Nature structural & molecular biology

authors： Schwartz S,Meshorer E,Ast G

更新日期：2009-09-01 00:00:00

abstract：:Advances in structure determination of the bacterial and eukaryotic transcription machinery have led to a marked increase in the understanding of the mechanism of transcription. Models for the specific assembly of the RNA polymerase II transcription machinery at a promoter, conformational changes that occur during ini...

journal_title：Nature structural & molecular biology

authors： Hahn S

更新日期：2004-05-01 00:00:00