Abstract:
:To our knowledge, no structural study to date has characterized, in an intact receptor, the coupling of conformational change in extracellular domains through a single-pass transmembrane domain to conformational change in cytoplasmic domains. Here we examine such coupling, and its unexpected complexity, using nearly full-length epidermal growth factor receptor (EGFR) and negative-stain EM. The liganded, dimeric EGFR ectodomain can couple both to putatively active, asymmetrically associated kinase dimers and to putatively inactive, symmetrically associated kinase dimers and monomers. Inhibitors that stabilize the active or inactive conformation of the kinase active site, as well as mutations in the kinase dimer interface and a juxtamembrane phosphorylation site, shift the equilibrium among the three kinase association states. This coupling of one conformation of an activated receptor ectodomain to multiple kinase-domain arrangements reveals previously unanticipated complexity in transmembrane signaling and facilitates regulation of receptor function in the juxtamembrane and cytoplasmic environments.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Mi LZ,Lu C,Li Z,Nishida N,Walz T,Springer TAdoi
10.1038/nsmb.2092subject
Has Abstractpub_date
2011-08-07 00:00:00pages
984-9issue
9eissn
1545-9993issn
1545-9985pii
nsmb.2092journal_volume
18pub_type
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