Abstract：
:In eukaryotic cells, many introns are constitutively, rather than alternatively, spliced and therefore do not contribute to isoform diversification. It has remained unclear what functional roles such constitutive splicing provides. To explore this issue, we asked how splicing affects the efficiency with which individual pre-messenger RNA transcripts are productively processed across different gene expression levels. We developed a quantitative single-molecule fluorescence in situ hybridization-based method to quantify splicing efficiency at transcription active sites in single cells. We found that both natural and synthetic genes in mouse and human cells exhibited an unexpected 'economy of scale' behavior in which splicing efficiency increased with transcription rate. Correlations between splicing efficiency and spatial proximity to nuclear speckles could explain this counterintuitive behavior. Functionally, economy of scale splicing represents a non-linear filter that amplifies the expression of genes when they are more strongly transcribed. These results indicate that constitutive splicing plays an active functional role in modulating gene expression.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Ding F,Elowitz MBdoi
10.1038/s41594-019-0226-xsubject
Has Abstractpub_date
2019-06-01 00:00:00pages
424-432issue
6eissn
1545-9993issn
1545-9985pii
10.1038/s41594-019-0226-xjournal_volume
26pub_type
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pub_type： 已发布勘误
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