Nemo kinase phosphorylates β-catenin to promote ommatidial rotation and connects core PCP factors to E-cadherin-β-catenin.

abstract：:The function of human XPA protein, a key subunit of the nucleotide excision repair pathway, has been examined with site-directed substitutions in its putative DNA-binding cleft. After screening for repair activity in a host-cell reactivation assay, we analyzed mutants by comparing their affinities for different substr...

journal_title：Nature structural & molecular biology

authors： Camenisch U,Dip R,Schumacher SB,Schuler B,Naegeli H

更新日期：2006-03-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression, including type I interferon production, by promoting host mRNA degradation and inhibiting host translation, in infected cells. We present evidence that nsp1 uses a novel, two-pronged strategy to inhibit host translation and gen...

journal_title：Nature structural & molecular biology

authors： Kamitani W,Huang C,Narayanan K,Lokugamage KG,Makino S

更新日期：2009-11-01 00:00:00

abstract：:Chromatin is a dynamic structure that must respond to myriad stimuli to regulate access to DNA, and chemical modification of histones is a major means by which the cell modulates nucleosome mobility and turnover. Histone modifications are linked to essentially every cellular process requiring DNA access, including tra...

journal_title：Nature structural & molecular biology

authors： Zentner GE,Henikoff S

更新日期：2013-03-01 00:00:00

abstract：:Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-...

journal_title：Nature structural & molecular biology

authors： Diez M,Zimmermann B,Börsch M,König M,Schweinberger E,Steigmiller S,Reuter R,Felekyan S,Kudryavtsev V,Seidel CA,Gräber P

更新日期：2004-02-01 00:00:00

abstract：:The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of n...

journal_title：Nature structural & molecular biology

authors： Quistgaard EM,Madsen P,Grøftehauge MK,Nissen P,Petersen CM,Thirup SS

更新日期：2009-01-01 00:00:00

abstract：:The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates heterogeneous microtubules and affects their functions. Here we describe the crystal and solution structure of the tubulin carboxypeptidase complex between vasohibin (VASH1) and small vasohibin-binding protein (SVBP), which fol...

journal_title：Nature structural & molecular biology

authors： Adamopoulos A,Landskron L,Heidebrecht T,Tsakou F,Bleijerveld OB,Altelaar M,Nieuwenhuis J,Celie PHN,Brummelkamp TR,Perrakis A

更新日期：2019-07-01 00:00:00

abstract：:Protein quality control depends on the tight regulation of interactions between molecular chaperones and polypeptide substrates. Substrate release from the chaperone Hsp70 is triggered by nucleotide-exchange factors (NEFs) that control folding and degradation fates via poorly understood mechanisms. We found that the a...

journal_title：Nature structural & molecular biology

authors： Gowda NKC,Kaimal JM,Kityk R,Daniel C,Liebau J,Öhman M,Mayer MP,Andréasson C

更新日期：2018-01-01 00:00:00

abstract：:We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-...

journal_title：Nature structural & molecular biology

authors： Taggart AJ,DeSimone AM,Shih JS,Filloux ME,Fairbrother WG

更新日期：2012-06-17 00:00:00

abstract：:Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by c...

journal_title：Nature structural & molecular biology

authors： Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

更新日期：2006-08-01 00:00:00

abstract：:The proteasome is the main ATP-dependent protease in eukaryotic cells and controls the concentration of many regulatory proteins in the cytosol and nucleus. Proteins are targeted to the proteasome by the covalent attachment of polyubiquitin chains. The ubiquitin modification serves as the proteasome recognition elemen...

journal_title：Nature structural & molecular biology

authors： Prakash S,Tian L,Ratliff KS,Lehotzky RE,Matouschek A

更新日期：2004-09-01 00:00:00

abstract：:Allosteric proteins transition among different conformational states in a ligand-dependent manner. Upon resolution of a protein's individual states, one can determine the probabilities of these states, thereby dissecting the energetic mechanisms underlying their conformational changes. Here we examine individual regul...

journal_title：Nature structural & molecular biology

authors： Lewis JH,Lu Z

更新日期：2019-09-01 00:00:00

abstract：:Biogenesis of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits, requires assembly chaperones. Here we analyzed the role of Rubisco accumulation factor1 (Raf1), a dimer of ∼40-kDa subunits. We find that Raf1 from Synechococcus elongatus acts downstream of chaperonin-ass...

journal_title：Nature structural & molecular biology

authors： Hauser T,Bhat JY,Miličić G,Wendler P,Hartl FU,Bracher A,Hayer-Hartl M

更新日期：2015-09-01 00:00:00

abstract：:The Rbfox family of splicing factors regulate alternative splicing during animal development and in disease, impacting thousands of exons in the maturing brain, heart and muscle. Rbfox proteins have long been known to bind to the RNA sequence GCAUG with high affinity and specificity, but just half of Rbfox binding sit...

journal_title：Nature structural & molecular biology

authors： Begg BE,Jens M,Wang PY,Minor CM,Burge CB

更新日期：2020-10-01 00:00:00

abstract：:Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Br...

journal_title：Nature structural & molecular biology

authors： Ke J,Chen RZ,Ban T,Zhou XE,Gu X,Tan MH,Chen C,Kang Y,Brunzelle JS,Zhu JK,Melcher K,Xu HE

更新日期：2013-12-01 00:00:00

abstract：:Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-ind...

journal_title：Nature structural & molecular biology

authors： Bühler M,Steiner S,Mohn F,Paillusson A,Mühlemann O

更新日期：2006-05-01 00:00:00

abstract：:Cholesterol-dependent cytolysins are bacterial protein toxins that bind to cholesterol-containing membranes, form oligomeric complexes and insert into the bilayer to create large aqueous pores. Membrane-dependent structural rearrangements required to initiate the oligomerization of perfringolysin O monomers have been ...

journal_title：Nature structural & molecular biology

authors： Ramachandran R,Tweten RK,Johnson AE

更新日期：2004-08-01 00:00:00

abstract：:Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification ...

journal_title：Nature structural & molecular biology

authors： Sellers JR,Veigel C

更新日期：2010-05-01 00:00:00

abstract：:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatch...

journal_title：Nature structural & molecular biology

authors： Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

更新日期：2010-07-01 00:00:00

abstract：:In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the ...

journal_title：Nature structural & molecular biology

authors： Yoshizawa S,Rasubala L,Ose T,Kohda D,Fourmy D,Maenaka K

更新日期：2005-02-01 00:00:00

abstract：:SUMO processing and deconjugation are essential proteolytic activities for nuclear metabolism and cell-cycle progression in yeast and higher eukaryotes. To elucidate the mechanisms used during substrate lysine deconjugation, SUMO isoform processing and SUMO isoform interactions, X-ray structures were determined for a ...

journal_title：Nature structural & molecular biology

authors： Reverter D,Lima CD

更新日期：2006-12-01 00:00:00

abstract：:Kinases are involved in disease development and modulation of their activity can be therapeutically beneficial. Drug-resistant mutant kinases are valuable tools in drug discovery efforts, but the prediction of mutants across the kinome is challenging. Here, we generate deep mutational scanning data to identify mutant ...

journal_title：Nature structural & molecular biology

authors： Persky NS,Hernandez D,Do Carmo M,Brenan L,Cohen O,Kitajima S,Nayar U,Walker A,Pantel S,Lee Y,Cordova J,Sathappa M,Zhu C,Hayes TK,Ram P,Pancholi P,Mikkelsen TS,Barbie DA,Yang X,Haq R,Piccioni F,Root DE,Johannes

更新日期：2020-01-01 00:00:00

abstract：:p53 binds as a tetramer to DNA targets consisting of two decameric half-sites separated by a variable spacer. Here we present high-resolution crystal structures of complexes between p53 core-domain tetramers and DNA targets consisting of contiguous half-sites. In contrast to previously reported p53-DNA complexes that ...

journal_title：Nature structural & molecular biology

authors： Kitayner M,Rozenberg H,Rohs R,Suad O,Rabinovich D,Honig B,Shakked Z

更新日期：2010-04-01 00:00:00

abstract：:Primary microRNA cleavage by the Drosha-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the au...

journal_title：Nature structural & molecular biology

authors： Barad O,Mann M,Chapnik E,Shenoy A,Blelloch R,Barkai N,Hornstein E

更新日期：2012-05-13 00:00:00

abstract：:Thousands of long noncoding RNAs (lncRNAs) have been discovered, but their functional characterization has been slowed by a limited set of research tools. Here we review emerging RNA-centric methods to interrogate the intrinsic structure of lncRNAs as well as their genomic localization and biochemical partners. Unders...

journal_title：Nature structural & molecular biology

authors： Chu C,Spitale RC,Chang HY

更新日期：2015-01-01 00:00:00

abstract：:We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show...

journal_title：Nature structural & molecular biology

authors： Kortemme T,Joachimiak LA,Bullock AN,Schuler AD,Stoddard BL,Baker D

更新日期：2004-04-01 00:00:00

abstract：:The core of the neurotransmitter release machinery is formed by SNARE complexes, which bring the vesicle and plasma membranes together and are key for fusion, and by Munc18-1, which controls SNARE-complex formation and may also have a direct role in fusion. In addition, SNARE complex assembly is likely orchestrated by...

journal_title：Nature structural & molecular biology

authors： Rizo J,Rosenmund C

更新日期：2008-07-01 00:00:00

abstract：:Translation has a fundamental function in defining the fate of the transcribed genome. RNA-sequencing (RNA-seq) data enable the quantification of complex transcript mixtures, often detecting several transcript isoforms of unknown functions for one gene. Here, we describe ORFquant, a method to annotate and quantify tra...

journal_title：Nature structural & molecular biology

authors： Calviello L,Hirsekorn A,Ohler U

更新日期：2020-08-01 00:00:00

abstract：:TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by...

journal_title：Nature structural & molecular biology

authors： Price AJ,Marzetta F,Lammers M,Ylinen LM,Schaller T,Wilson SJ,Towers GJ,James LC

更新日期：2009-10-01 00:00:00

abstract：:The exosome is an RNA-decay complex that constantly monitors transcription and contributes to post-transcriptional turnover of faulty mRNAs. Yet how nuclear RNA surveillance by the exosome is coordinated with transcription is still unknown. Here we show that the RNA exosome of Schizosaccharomyces pombe can target the ...

journal_title：Nature structural & molecular biology

authors： Lemay JF,Larochelle M,Marguerat S,Atkinson S,Bähler J,Bachand F

更新日期：2014-10-01 00:00:00

abstract：:An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from h...

journal_title：Nature structural & molecular biology

authors： Schwartz S,Meshorer E,Ast G

更新日期：2009-09-01 00:00:00