当前位置： SCI文献检索 > NATURE STRUCTURAL & MOLECULAR BIOLOGY期刊下所有文献 > Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.

Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.

Abstract：

:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatches are accommodated among five of the gene products at the portal vertex. Reconstructions of infectious and empty particles show a conformational change of the 'valve' density in the nozzle, an orientation difference in the tail fibers, a disordering of the C terminus of the portal protein and the disappearance of the core proteins. In addition, cryo-electron tomography of P-SSP7 infecting Prochlorococcus showed the same tail-fiber conformation as that in empty particles. Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

doi

10.1038/nsmb.1823

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

830-6

issue

7

eissn

1545-9993

issn

1545-9985

pii

nsmb.1823

journal_volume

17

pub_type

杂志文章

相关文献

NATURE STRUCTURAL & MOLECULAR BIOLOGY文献大全
  • Molecular basis for RNA polymerization by Qβ replicase.

    abstract：:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that a...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2204

    authors： Takeshita D,Tomita K

    更新日期：2012-01-15 00:00:00

  • The WAVE regulatory complex is inhibited.

    abstract：:The WAVE regulatory complex (WRC) transmits information from the Rac GTPase to the actin nucleator Arp2/3 complex. We have reconstituted recombinant human and Drosophila WRC in several forms and shown that they are inactive toward Arp2/3 complex but can be activated by Rac in a nucleotide-dependent fashion. Our observ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1587

    authors： Ismail AM,Padrick SB,Chen B,Umetani J,Rosen MK

    更新日期：2009-05-01 00:00:00

  • A teacupful of medicine?

    abstract：:Green tea has held a long-standing place in traditional Asian medicine. Scientific research is now beginning to explain why. ...

    journal_title：Nature structural & molecular biology

    pub_type： 评论,社论

    doi：10.1038/nsmb0608-537

    authors：

    更新日期：2008-06-01 00:00:00

  • Catching DNA with hoops-biophysical approaches to clarify the mechanism of SMC proteins.

    abstract：:Structural maintenance of chromosome (SMC) complexes are central regulators of chromosome architecture that are essential in all domains of life. For decades, the structural biology field has been debating how these conserved protein complexes use their intricate ring-like structures to structurally organize DNA. Here...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb.3507

    authors： Eeftens J,Dekker C

    更新日期：2017-12-07 00:00:00

  • Interaction between the keratin cytoskeleton and eEF1Bgamma affects protein synthesis in epithelial cells.

    abstract：:Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its gamma subunit (eEF1Bgamma) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by approximately 20% and selectively increases 80S ribosom...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1301

    authors： Kim S,Kellner J,Lee CH,Coulombe PA

    更新日期：2007-10-01 00:00:00

  • Antisense transcripts are targets for activating small RNAs.

    abstract：:Agents that activate expression of specific genes to probe cellular pathways or alleviate disease would go beyond existing approaches for controlling gene expression. Duplex RNAs complementary to promoter regions can repress or activate gene expression. The mechanism of these promoter-directed antigene RNAs (agRNAs) h...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1444

    authors： Schwartz JC,Younger ST,Nguyen NB,Hardy DB,Monia BP,Corey DR,Janowski BA

    更新日期：2008-08-01 00:00:00

  • EJC-independent degradation of nonsense immunoglobulin-mu mRNA depends on 3' UTR length.

    abstract：:Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-ind...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1081

    authors： Bühler M,Steiner S,Mohn F,Paillusson A,Mühlemann O

    更新日期：2006-05-01 00:00:00

  • Computational redesign of protein-protein interaction specificity.

    abstract：:We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb749

    authors： Kortemme T,Joachimiak LA,Bullock AN,Schuler AD,Stoddard BL,Baker D

    更新日期：2004-04-01 00:00:00

  • Structure of the Dom34-Hbs1 complex and implications for no-go decay.

    abstract：:No-go decay (NGD) targets mRNAs with stalls in translation elongation for endonucleolytic cleavage in a process involving the Dom34 and Hbs1 proteins. The crystal structure of a Schizosaccharomyces pombe Dom34-Hbs1 complex reveals an overall shape similar to that of eRF1-eRF3-GTP and EF-Tu-tRNA-GDPNP. Similarly to eRF...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1922

    authors： Chen L,Muhlrad D,Hauryliuk V,Cheng Z,Lim MK,Shyp V,Parker R,Song H

    更新日期：2010-10-01 00:00:00

  • Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.

    abstract：:Point mutations in cysteine string protein-α (CSPα) cause dominantly inherited adult-onset neuronal ceroid lipofuscinosis (ANCL), a rapidly progressing and lethal neurodegenerative disease with no treatment. ANCL mutations are proposed to trigger CSPα aggregation/oligomerization, but the mechanism of oligomer formatio...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0375-y

    authors： Naseri NN,Ergel B,Kharel P,Na Y,Huang Q,Huang R,Dolzhanskaya N,Burré J,Velinov MT,Sharma M

    更新日期：2020-02-01 00:00:00

  • Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA.

    abstract：:In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA. Using purified components in the presence of ATP-γS, we have captured in vitro an intermediate in pre-RC assembly that contain...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2629

    authors： Sun J,Evrin C,Samel SA,Fernández-Cid A,Riera A,Kawakami H,Stillman B,Speck C,Li H

    更新日期：2013-08-01 00:00:00

  • Structural basis of the proinflammatory signaling complex mediated by TSLP.

    abstract：:Thymic stromal lymphopoietin (TSLP), a cytokine produced by epithelial cells at barrier surfaces, is pivotal for the development of widespread chronic inflammatory disorders such as asthma and atopic dermatitis. The structure of the mouse TSLP-mediated signaling complex reveals how TSLP establishes extensive interface...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2794

    authors： Verstraete K,van Schie L,Vyncke L,Bloch Y,Tavernier J,Pauwels E,Peelman F,Savvides SN

    更新日期：2014-04-01 00:00:00

  • Crystal structure of the ribosome recycling factor bound to the ribosome.

    abstract：:In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1282

    authors： Weixlbaumer A,Petry S,Dunham CM,Selmer M,Kelley AC,Ramakrishnan V

    更新日期：2007-08-01 00:00:00

  • Reconstitution of anaphase DNA bridge recognition and disjunction.

    abstract：:Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0123-8

    authors： Sarlós K,Biebricher AS,Bizard AH,Bakx JAM,Ferreté-Bonastre AG,Modesti M,Paramasivam M,Yao Q,Peterman EJG,Wuite GJL,Hickson ID

    更新日期：2018-09-01 00:00:00

  • Structural basis for the coevolution of a viral RNA-protein complex.

    abstract：:The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to s...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1327

    authors： Chao JA,Patskovsky Y,Almo SC,Singer RH

    更新日期：2008-01-01 00:00:00

  • Let K-Ras activate its own inhibitor.

    abstract：: ...

    journal_title：Nature structural & molecular biology

    pub_type： 新闻

    doi：10.1038/s41594-018-0066-0

    authors： Statsyuk AV

    更新日期：2018-06-01 00:00:00

  • Synaptic vesicle fusion.

    abstract：:The core of the neurotransmitter release machinery is formed by SNARE complexes, which bring the vesicle and plasma membranes together and are key for fusion, and by Munc18-1, which controls SNARE-complex formation and may also have a direct role in fusion. In addition, SNARE complex assembly is likely orchestrated by...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb.1450

    authors： Rizo J,Rosenmund C

    更新日期：2008-07-01 00:00:00

  • Crystal structure of the tubulin tyrosine carboxypeptidase complex VASH1-SVBP.

    abstract：:The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates heterogeneous microtubules and affects their functions. Here we describe the crystal and solution structure of the tubulin carboxypeptidase complex between vasohibin (VASH1) and small vasohibin-binding protein (SVBP), which fol...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-019-0254-6

    authors： Adamopoulos A,Landskron L,Heidebrecht T,Tsakou F,Bleijerveld OB,Altelaar M,Nieuwenhuis J,Celie PHN,Brummelkamp TR,Perrakis A

    更新日期：2019-07-01 00:00:00

  • Physical and functional coupling of RNA-dependent RNA polymerase and Dicer in the biogenesis of endogenous siRNAs.

    abstract：:Many classes of small RNA (sRNA) involved in RNA silencing are generated by double-stranded RNA (dsRNA) processing. Although principles of sRNA biogenesis have emerged, newly identified classes of sRNAs have features that suggest additional biogenesis mechanisms. Tetrahymena thermophila expresses one such class, compr...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1262

    authors： Lee SR,Collins K

    更新日期：2007-07-01 00:00:00

  • Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.

    abstract：:The exon-junction complex (EJC) functionally links splicing to subsequent mRNA localization, translation and stability. Sequence-independent binding of the EJC core to RNA is ensured by the DEAD-box helicase eIF4AIII. Here, we identified the splicing factor CWC22 as a new eIF4AIII partner in flies and humans. CWC22 co...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2380

    authors： Barbosa I,Haque N,Fiorini F,Barrandon C,Tomasetto C,Blanchette M,Le Hir H

    更新日期：2012-10-01 00:00:00

  • Ribosome-associated protein quality control.

    abstract：:Protein synthesis by the ribosome can fail for numerous reasons including faulty mRNA, insufficient availability of charged tRNAs and genetic errors. All organisms have evolved mechanisms to recognize stalled ribosomes and initiate pathways for recycling, quality control and stress signaling. Here we review the discov...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb.3147

    authors： Brandman O,Hegde RS

    更新日期：2016-01-01 00:00:00

  • Author Correction: Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature structural & molecular biology

    pub_type： 已发布勘误

    doi：10.1038/s41594-020-0498-1

    authors： Mahalingan KK,Keith Keenan E,Strickland M,Li Y,Liu Y,Ball HL,Tanner ME,Tjandra N,Roll-Mecak A

    更新日期：2020-09-01 00:00:00

  • STAT2 is an essential adaptor in USP18-mediated suppression of type I interferon signaling.

    abstract：:Type I interferons (IFNs) are multifunctional cytokines that regulate immune responses and cellular functions but also can have detrimental effects on human health. A tight regulatory network therefore controls IFN signaling, which in turn may interfere with medical interventions. The JAK-STAT signaling pathway transm...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3378

    authors： Arimoto KI,Löchte S,Stoner SA,Burkart C,Zhang Y,Miyauchi S,Wilmes S,Fan JB,Heinisch JJ,Li Z,Yan M,Pellegrini S,Colland F,Piehler J,Zhang DE

    更新日期：2017-03-01 00:00:00

  • Telomere position effect regulates DUX4 in human facioscapulohumeral muscular dystrophy.

    abstract：:Telomeres may regulate human disease by at least two independent mechanisms. First, replicative senescence occurs once short telomeres generate DNA-damage signals that produce a barrier to tumor progression. Second, telomere position effects (TPE) could change gene expression at intermediate telomere lengths in cultur...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2571

    authors： Stadler G,Rahimov F,King OD,Chen JC,Robin JD,Wagner KR,Shay JW,Emerson CP Jr,Wright WE

    更新日期：2013-06-01 00:00:00

  • SR protein kinases promote splicing of nonconsensus introns.

    abstract：:Phosphorylation of the spliceosome is essential for RNA splicing, yet how and to what extent kinase signaling affects splicing have not been defined on a genome-wide basis. Using a chemical genetic approach, we show in Schizosaccharomyces pombe that the SR protein kinase Dsk1 is required for efficient splicing of intr...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3057

    authors： Lipp JJ,Marvin MC,Shokat KM,Guthrie C

    更新日期：2015-08-01 00:00:00

  • Cabin1 restrains p53 activity on chromatin.

    abstract：:The tumor suppressor p53 has been proposed to bind target promoters upon genotoxic stress. However, recent evidence shows that p53 occupies some target promoters without such stress, suggesting that a negative regulator might render p53 transcriptionally inactive on these promoters. Here we show that calcineurin bindi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1657

    authors： Jang H,Choi SY,Cho EJ,Youn HD

    更新日期：2009-09-01 00:00:00

  • Structural basis for the recognition of N-end rule substrates by the UBR box of ubiquitin ligases.

    abstract：:The N-end rule pathway is a regulated proteolytic system that targets proteins containing destabilizing N-terminal residues (N-degrons) for ubiquitination and proteasomal degradation in eukaryotes. The N-degrons of type 1 substrates contain an N-terminal basic residue that is recognized by the UBR box domain of the E3...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1907

    authors： Choi WS,Jeong BC,Joo YJ,Lee MR,Kim J,Eck MJ,Song HK

    更新日期：2010-10-01 00:00:00

  • The Anopheles-midgut APN1 structure reveals a new malaria transmission-blocking vaccine epitope.

    abstract：:Mosquito-based malaria transmission-blocking vaccines (mTBVs) target midgut-surface antigens of the Plasmodium parasite's obligate vector, the Anopheles mosquito. The alanyl aminopeptidase N (AnAPN1) is the leading mTBV immunogen; however, AnAPN1's role in Plasmodium infection of the mosquito and how anti-AnAPN1 antib...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3048

    authors： Atkinson SC,Armistead JS,Mathias DK,Sandeu MM,Tao D,Borhani-Dizaji N,Tarimo BB,Morlais I,Dinglasan RR,Borg NA

    更新日期：2015-07-01 00:00:00

  • Structural basis for the molecular evolution of SRP-GTPase activation by protein.

    abstract：:Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviat...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2141

    authors： Bange G,Kümmerer N,Grudnik P,Lindner R,Petzold G,Kressler D,Hurt E,Wild K,Sinning I

    更新日期：2011-11-06 00:00:00

  • A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction.

    abstract：:The CDK8 kinase module (CKM) is a conserved, dissociable Mediator subcomplex whose component subunits were genetically linked to the RNA polymerase II (RNAPII) C-terminal domain (CTD) and individually recognized as transcriptional repressors before Mediator was identified as a pre-eminent complex in eukaryotic transcr...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2549

    authors： Tsai KL,Sato S,Tomomori-Sato C,Conaway RC,Conaway JW,Asturias FJ

    更新日期：2013-05-01 00:00:00