Abstract：
:Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Brachypodium distachyon THA8 either free of RNA or bound to two of the identified RNA sites. The apostructure reveals a THA8 monomer with five tandem PPR repeats arranged in a planar fold. The complexes of THA8 bound to the two short RNA fragments surprisingly reveal asymmetric THA8 dimers with the bound RNAs at the dimeric interface. RNA binding induces THA8 dimerization, with a conserved G nucleotide of the bound RNAs making extensive contacts with both monomers. Together, these results establish a new model of RNA recognition by RNA-induced formation of an asymmetric dimer of a PPR protein.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Ke J,Chen RZ,Ban T,Zhou XE,Gu X,Tan MH,Chen C,Kang Y,Brunzelle JS,Zhu JK,Melcher K,Xu HEdoi
10.1038/nsmb.2710subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1377-82issue
12eissn
1545-9993issn
1545-9985pii
nsmb.2710journal_volume
20pub_type
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