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Direct sequencing of Arabidopsis thaliana RNA reveals patterns of cleavage and polyadenylation.

Abstract：

:It has recently been shown that RNA 3'-end formation plays a more widespread role in controlling gene expression than previously thought. To examine the impact of regulated 3'-end formation genome-wide, we applied direct RNA sequencing to A. thaliana. Here we show the authentic transcriptome in unprecedented detail and describe the effects of 3'-end formation on genome organization. We reveal extreme heterogeneity in RNA 3' ends, discover previously unrecognized noncoding RNAs and propose widespread reannotation of the genome. We explain the origin of most poly(A)(+) antisense RNAs and identify cis elements that control 3'-end formation in different registers. These findings are essential to understanding what the genome actually encodes, how it is organized and how regulated 3'-end formation affects these processes.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Sherstnev A,Duc C,Cole C,Zacharaki V,Hornyik C,Ozsolak F,Milos PM,Barton GJ,Simpson GG

doi

10.1038/nsmb.2345

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

845-52

issue

8

eissn

1545-9993

issn

1545-9985

pii

nsmb.2345

journal_volume

19

pub_type

杂志文章

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