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Mapping the sequence of conformational changes underlying selectivity filter gating in the K(v)11.1 potassium channel.

Abstract：

:The potassium channel selectivity filter both discriminates between K(+) and sodium ions and contributes to gating of ion flow. Static structures of conducting (open) and nonconducting (inactivated) conformations of this filter are known; however, the sequence of protein rearrangements that connect these two states is not. We show that closure of the selectivity filter gate in the human K(v)11.1 K(+) channel (also known as hERG, for ether-a-go-go-related gene), a key regulator of the rhythm of the heartbeat, is initiated by K(+) exit, followed in sequence by conformational rearrangements of the pore domain outer helix, extracellular turret region, voltage sensor domain, intracellular domains and pore domain inner helix. In contrast to the simple wave-like sequence of events proposed for opening of ligand-gated ion channels, a complex spatial and temporal sequence of widespread domain motions connect the open and inactivated states of the K(v)11.1 K(+) channel.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Wang DT,Hill AP,Mann SA,Tan PS,Vandenberg JI

doi

10.1038/nsmb.1966

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

35-41

issue

1

eissn

1545-9993

issn

1545-9985

pii

nsmb.1966

journal_volume

18

pub_type

杂志文章

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