Abstract:
:We present a structural analysis of the folding transition states of three SH3 domains. Our results reveal that the secondary structure is not yet fully formed at this stage of folding and that the solvent is only partially excluded from the interior of the protein. Comparison of the members of the transition state ensemble with a database of native folds shows that, despite substantial local variability, the transition state structures can all be classified as having the topology characteristic of an SH3 domain. Our results suggest a mechanism for folding in which the formation of a network of interactions among a subset of hydrophobic residues ensures that the native topology is generated. Such a mechanism enables high fidelity in folding while minimizing the need to establish a large number of specific interactions in the conformational search.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Lindorff-Larsen K,Vendruscolo M,Paci E,Dobson CMdoi
10.1038/nsmb765keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
443-9issue
5eissn
1545-9993issn
1545-9985pii
nsmb765journal_volume
11pub_type
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