Structural basis for DNA bridging by barrier-to-autointegration factor.

abstract：:Type I interferons (IFNs) are multifunctional cytokines that regulate immune responses and cellular functions but also can have detrimental effects on human health. A tight regulatory network therefore controls IFN signaling, which in turn may interfere with medical interventions. The JAK-STAT signaling pathway transm...

journal_title：Nature structural & molecular biology

authors： Arimoto KI,Löchte S,Stoner SA,Burkart C,Zhang Y,Miyauchi S,Wilmes S,Fan JB,Heinisch JJ,Li Z,Yan M,Pellegrini S,Colland F,Piehler J,Zhang DE

更新日期：2017-03-01 00:00:00

abstract：:We describe a novel approach to separate two ribosome populations from the same cells and use this method in combination with RNA-seq to identify mRNAs bound to Saccharomyces cerevisiae ribosomes with and without Rps26, a protein linked to the pathogenesis of Diamond-Blackfan anemia (DBA). These analyses reveal that R...

journal_title：Nature structural & molecular biology

authors： Ferretti MB,Ghalei H,Ward EA,Potts EL,Karbstein K

更新日期：2017-09-01 00:00:00

abstract：:Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can ...

journal_title：Nature structural & molecular biology

authors： Sarlós K,Biebricher AS,Bizard AH,Bakx JAM,Ferreté-Bonastre AG,Modesti M,Paramasivam M,Yao Q,Peterman EJG,Wuite GJL,Hickson ID

更新日期：2018-09-01 00:00:00

abstract：:The monoclonal antibody 13H11 shares part of its epitope in the HIV-1 gp41 membrane-proximal external region (MPER) with the rare, broadly neutralizing human antibody 2F5. Although 13H11 partially cross-blocked 2F5 binding, 13H11 is non-neutralizing and does not block 2F5 neutralization. We show that unlike 2F5, 13H11...

journal_title：Nature structural & molecular biology

authors： Nicely NI,Dennison SM,Spicer L,Scearce RM,Kelsoe G,Ueda Y,Chen H,Liao HX,Alam SM,Haynes BF

更新日期：2010-12-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:The highly contagious measles virus infects millions of individuals worldwide, causing serious disease in children of developing countries. Infection is initiated by attachment of the measles virus hemagglutinin (MV-H), a glycoprotein anchored to the virus envelope, to the host cell receptors CD46 or signaling lymphoc...

journal_title：Nature structural & molecular biology

authors： Santiago C,Celma ML,Stehle T,Casasnovas JM

更新日期：2010-01-01 00:00:00

abstract：:During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1...

journal_title：Nature structural & molecular biology

authors： Ito K,Murayama Y,Takahashi M,Iwasaki H

更新日期：2018-01-01 00:00:00

abstract：:Mosquito-based malaria transmission-blocking vaccines (mTBVs) target midgut-surface antigens of the Plasmodium parasite's obligate vector, the Anopheles mosquito. The alanyl aminopeptidase N (AnAPN1) is the leading mTBV immunogen; however, AnAPN1's role in Plasmodium infection of the mosquito and how anti-AnAPN1 antib...

journal_title：Nature structural & molecular biology

authors： Atkinson SC,Armistead JS,Mathias DK,Sandeu MM,Tao D,Borhani-Dizaji N,Tarimo BB,Morlais I,Dinglasan RR,Borg NA

更新日期：2015-07-01 00:00:00

abstract：:Tripeptidyl peptidase II (TPP II) is the largest known eukaryotic protease (6 MDa). It is believed to act downstream of the 26S proteasome, cleaving tripeptides from the N termini of longer peptides, and it is implicated in numerous cellular processes. Here we report the structure of Drosophila TPP II determined by a ...

journal_title：Nature structural & molecular biology

authors： Chuang CK,Rockel B,Seyit G,Walian PJ,Schönegge AM,Peters J,Zwart PH,Baumeister W,Jap BK

更新日期：2010-08-01 00:00:00

abstract：:Newly synthesized polypeptides undergo various cotranslational maturation steps, including N-terminal enzymatic processing, chaperone-assisted folding and membrane targeting, but the spatial and temporal coordination of these steps is unclear. We show that Escherichia coli methionine aminopeptidase (MAP) associates wi...

journal_title：Nature structural & molecular biology

authors： Sandikci A,Gloge F,Martinez M,Mayer MP,Wade R,Bukau B,Kramer G

更新日期：2013-07-01 00:00:00

abstract：:Small-molecule BET inhibitors interfere with the epigenetic interactions between acetylated histones and the bromodomains of the BET family proteins, including BRD4, and they potently inhibit growth of malignant cells by targeting cancer-promoting genes. BRD4 interacts with the pause-release factor P-TEFb and has been...

journal_title：Nature structural & molecular biology

authors： Kanno T,Kanno Y,LeRoy G,Campos E,Sun HW,Brooks SR,Vahedi G,Heightman TD,Garcia BA,Reinberg D,Siebenlist U,O'Shea JJ,Ozato K

更新日期：2014-12-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Huo J,Le Bas A,Ruza RR,Duyvesteyn HME,Mikolajek H,Malinauskas T,Tan TK,Rijal P,Dumoux M,Ward PN,Ren J,Zhou D,Harrison PJ,Weckener M,Clare DK,Vogirala VK,Radecke J,Moynié L,Zhao Y,Gilbert-Jaramillo J,Knight ML,Tr

更新日期：2020-11-01 00:00:00

abstract：:TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by...

journal_title：Nature structural & molecular biology

authors： Price AJ,Marzetta F,Lammers M,Ylinen LM,Schaller T,Wilson SJ,Towers GJ,James LC

更新日期：2009-10-01 00:00:00

abstract：:The noncoding RNA Xist recruits silencing factors to the inactive X chromosome (Xi) and facilitates re-organization of Xi structure. Here, we examine the mouse epigenomic landscape of Xi and assess how Xist alters chromatin accessibility. Xist deletion triggers a gain of accessibility of select chromatin regions that ...

journal_title：Nature structural & molecular biology

authors： Jégu T,Blum R,Cochrane JC,Yang L,Wang CY,Gilles ME,Colognori D,Szanto A,Marr SK,Kingston RE,Lee JT

更新日期：2019-02-01 00:00:00

abstract：:The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have been shown to be recruited through the core to reach Rrp44's exo-RNase (EXO) site. Using single-particle EM and biochem...

journal_title：Nature structural & molecular biology

authors： Liu JJ,Bratkowski MA,Liu X,Niu CY,Ke A,Wang HW

更新日期：2014-01-01 00:00:00

abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

journal_title：Nature structural & molecular biology

authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

更新日期：2012-06-24 00:00:00

abstract：:The CagA protein of Helicobacter pylori interacts with numerous cellular factors and is associated with increased virulence and risk of gastric carcinoma. We present here the cocrystal structure of a subdomain of CagA with the human kinase PAR1b/MARK2, revealing that a CagA peptide mimics substrates of this kinase fam...

journal_title：Nature structural & molecular biology

authors： Nesić D,Miller MC,Quinkert ZT,Stein M,Chait BT,Stebbins CE

更新日期：2010-01-01 00:00:00

abstract：:Many aspects of nucleic acid metabolism, such as DNA replication, repair and transcription, are regulated by the post-translational modifiers ubiquitin and SUMO. Not surprisingly, DNA itself plays an integral part in determining the modification of most chromatin-associated targets. Conversely, ubiquitination or SUMOy...

journal_title：Nature structural & molecular biology

authors： Ulrich HD

更新日期：2014-04-01 00:00:00

abstract：:Advances in structure determination of the bacterial and eukaryotic transcription machinery have led to a marked increase in the understanding of the mechanism of transcription. Models for the specific assembly of the RNA polymerase II transcription machinery at a promoter, conformational changes that occur during ini...

journal_title：Nature structural & molecular biology

authors： Hahn S

更新日期：2004-05-01 00:00:00

abstract：:Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification ...

journal_title：Nature structural & molecular biology

authors： Sellers JR,Veigel C

更新日期：2010-05-01 00:00:00

abstract：:Processing of microRNAs (miRNAs) from their precursors to their biologically active mature forms is regulated during development and cancer. We show that mouse pri- or pre-miR-151 can bind to and compete with mature miR-151-5p and miR-151-3p for binding sites contained within the complementary regions of the E2f6 mRNA...

journal_title：Nature structural & molecular biology

authors： Roy-Chaudhuri B,Valdmanis PN,Zhang Y,Wang Q,Luo QJ,Kay MA

更新日期：2014-09-01 00:00:00

abstract：:Pre-messenger RNAs (pre-mRNAs) maturation is initiated cotranscriptionally. It is therefore conceivable that chromatin-borne information participates in alternative splicing. Here we find that elevated levels of trimethylation of histone H3 on Lys9 (H3K9me3) are a characteristic of the alternative exons of several gen...

journal_title：Nature structural & molecular biology

authors： Saint-André V,Batsché E,Rachez C,Muchardt C

更新日期：2011-03-01 00:00:00

abstract：:Herpesviruses, which cause many incurable diseases, infect cells by fusing viral and cellular membranes. Whereas most other enveloped viruses use a single viral catalyst called a fusogen, herpesviruses, inexplicably, require two conserved fusion-machinery components, gB and the heterodimer gH-gL, plus other nonconserv...

journal_title：Nature structural & molecular biology

authors： Chowdary TK,Cairns TM,Atanasiu D,Cohen GH,Eisenberg RJ,Heldwein EE

更新日期：2010-07-01 00:00:00

abstract：:Beta-2 microglobulin (beta2m) is a globular protein that self-associates into fibrillar amyloid deposits in patients undergoing hemodialysis therapy. Formation of these beta-sheet-rich assemblies is a fundamental property of polypeptides that can be triggered by diverse conditions. For beta2m, oligomerization into pre...

journal_title：Nature structural & molecular biology

authors： Calabrese MF,Eakin CM,Wang JM,Miranker AD

更新日期：2008-09-01 00:00:00

abstract：:Cells respond to changes in environmental conditions via orchestrated modifications in gene expression. For example, in response to heat shock, cells execute a program of gene-specific transcriptional activation and repression. Although the activation of genes upon heat shock has been widely studied, the mechanism of ...

journal_title：Nature structural & molecular biology

authors： Allen TA,Von Kaenel S,Goodrich JA,Kugel JF

更新日期：2004-09-01 00:00:00

abstract：:The function of human XPA protein, a key subunit of the nucleotide excision repair pathway, has been examined with site-directed substitutions in its putative DNA-binding cleft. After screening for repair activity in a host-cell reactivation assay, we analyzed mutants by comparing their affinities for different substr...

journal_title：Nature structural & molecular biology

authors： Camenisch U,Dip R,Schumacher SB,Schuler B,Naegeli H

更新日期：2006-03-01 00:00:00

abstract：:Bacteriophages express proteins that inactivate the CRISPR-Cas bacterial immune system. Here we report the crystal structure of the anti-CRISPR protein AcrF3 in complex with Pseudomonas aeruginosa Cas3 (PaCas3). AcrF3 forms a homodimer that locks PaCas3 in an ADP-bound form, blocks the entrance of the DNA-binding tunn...

journal_title：Nature structural & molecular biology

authors： Wang X,Yao D,Xu JG,Li AR,Xu J,Fu P,Zhou Y,Zhu Y

更新日期：2016-09-01 00:00:00

abstract：:The extracellular domain of the metabotropic glutamate receptor 1alpha (mGluR1alpha) forms a dimer and the ligand, glutamate, induces a structural rearrangement in this domain. However, the conformational change in the cytoplasmic domain, which is critical for mGluR1alpha's interaction with G proteins, remains unclear...

journal_title：Nature structural & molecular biology

authors： Tateyama M,Abe H,Nakata H,Saito O,Kubo Y

更新日期：2004-07-01 00:00:00

abstract：:Many viruses bypass canonical cap-dependent translation in host cells by using internal ribosomal entry sites (IRESs) in their transcripts; IRESs hijack initiation factors for the assembly of initiation complexes. However, it is currently unknown how IRES RNAs recognize initiation factors that have no endogenous RNA b...

journal_title：Nature structural & molecular biology

authors： Imai S,Kumar P,Hellen CU,D'Souza VM,Wagner G

更新日期：2016-09-01 00:00:00

abstract：:Argonaute (Ago) proteins mediate silencing of nucleic acid targets by small RNAs. In fission yeast, Ago1, Tas3 and Chp1 assemble into a RITS complex, which silences transcription near centromeres. Here we describe a repetitive motif within Tas3, termed the 'Argonaute hook', that is conserved from yeast to humans and b...

journal_title：Nature structural & molecular biology

authors： Till S,Lejeune E,Thermann R,Bortfeld M,Hothorn M,Enderle D,Heinrich C,Hentze MW,Ladurner AG

更新日期：2007-10-01 00:00:00