Crystal structures of STING protein reveal basis for recognition of cyclic di-GMP.

abstract：:Integral membrane proteins are generally believed to have unique membrane topologies. However, it has been suggested that dual-topology proteins that adopt a mixture of two opposite orientations in the membrane may exist. Here we show that the membrane orientations of five dual-topology candidates identified in Escher...

journal_title：Nature structural & molecular biology

authors： Rapp M,Granseth E,Seppälä S,von Heijne G

更新日期：2006-02-01 00:00:00

abstract：:Despite growing appreciation of the importance of long noncoding RNAs (lncRNAs) in normal physiology and disease, our knowledge of cancer-related lncRNAs remains limited. By repurposing microarray probes, we constructed expression profiles of 10,207 lncRNA genes in approximately 1,300 tumors over four different cancer...

journal_title：Nature structural & molecular biology

authors： Du Z,Fei T,Verhaak RG,Su Z,Zhang Y,Brown M,Chen Y,Liu XS

更新日期：2013-07-01 00:00:00

abstract：:The WAVE regulatory complex (WRC) transmits information from the Rac GTPase to the actin nucleator Arp2/3 complex. We have reconstituted recombinant human and Drosophila WRC in several forms and shown that they are inactive toward Arp2/3 complex but can be activated by Rac in a nucleotide-dependent fashion. Our observ...

journal_title：Nature structural & molecular biology

authors： Ismail AM,Padrick SB,Chen B,Umetani J,Rosen MK

更新日期：2009-05-01 00:00:00

abstract：:Chaperones increase the folding yields of soluble proteins by suppressing misfolding and aggregation, but how they modulate the folding of integral membrane proteins is not well understood. Here we use single-molecule force spectroscopy and NMR spectroscopy to observe the periplasmic holdase chaperones SurA and Skp sh...

journal_title：Nature structural & molecular biology

authors： Thoma J,Burmann BM,Hiller S,Müller DJ

更新日期：2015-10-01 00:00:00

abstract：:Self-templating assemblies of the human prion protein are clinically associated with transmissible spongiform encephalopathies. Here we present the cryo-EM structure of a denaturant- and protease-resistant fibril formed in vitro spontaneously by a 9.7-kDa unglycosylated fragment of the human prion protein. This human ...

journal_title：Nature structural & molecular biology

authors： Glynn C,Sawaya MR,Ge P,Gallagher-Jones M,Short CW,Bowman R,Apostol M,Zhou ZH,Eisenberg DS,Rodriguez JA

更新日期：2020-05-01 00:00:00

abstract：:Ribosomes catalyze the formation of peptide bonds between aminoacyl esters of transfer RNAs within a catalytic center composed of ribosomal RNA only. Here we show that the reaction of P-site formylmethionine (fMet)-tRNA(fMet) with a modified A-site tRNA substrate, Phelac-tRNA(Phe), in which the nucleophilic amino grou...

journal_title：Nature structural & molecular biology

authors： Bieling P,Beringer M,Adio S,Rodnina MV

更新日期：2006-05-01 00:00:00

abstract：:The function of human XPA protein, a key subunit of the nucleotide excision repair pathway, has been examined with site-directed substitutions in its putative DNA-binding cleft. After screening for repair activity in a host-cell reactivation assay, we analyzed mutants by comparing their affinities for different substr...

journal_title：Nature structural & molecular biology

authors： Camenisch U,Dip R,Schumacher SB,Schuler B,Naegeli H

更新日期：2006-03-01 00:00:00

abstract：:Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-...

journal_title：Nature structural & molecular biology

authors： Diez M,Zimmermann B,Börsch M,König M,Schweinberger E,Steigmiller S,Reuter R,Felekyan S,Kudryavtsev V,Seidel CA,Gräber P

更新日期：2004-02-01 00:00:00

abstract：:The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to s...

journal_title：Nature structural & molecular biology

authors： Chao JA,Patskovsky Y,Almo SC,Singer RH

更新日期：2008-01-01 00:00:00

abstract：:Microtubules are regulated by post-translational modifications of tubulin. The ligation and cleavage of the carboxy-terminal tyrosine of α-tubulin impact microtubule functions during mitosis, cardiomyocyte contraction and neuronal processes. Tubulin tyrosination and detyrosination are mediated by tubulin tyrosine liga...

journal_title：Nature structural & molecular biology

authors： Li F,Hu Y,Qi S,Luo X,Yu H

更新日期：2019-07-01 00:00:00

abstract：:Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can ...

journal_title：Nature structural & molecular biology

authors： Sarlós K,Biebricher AS,Bizard AH,Bakx JAM,Ferreté-Bonastre AG,Modesti M,Paramasivam M,Yao Q,Peterman EJG,Wuite GJL,Hickson ID

更新日期：2018-09-01 00:00:00

abstract：:Although R loops can occur at different genomic locations, the factors that determine their formation and frequency remain unclear. Emerging evidence indicates that DNA breaks stimulate DNA-RNA hybrid formation. Here, we discuss the possibility that formation of hybrids may be an inevitable risk of DNA breaks that occ...

journal_title：Nature structural & molecular biology

authors： Aguilera A,Gómez-González B

更新日期：2017-05-04 00:00:00

abstract：:Bacteriophage T4 and related viruses have a contractile tail that serves as an efficient mechanical device for infecting bacteria. A three-dimensional cryo-EM reconstruction of the mature T4 tail assembly at 15-A resolution shows the hexagonal dome-shaped baseplate, the extended contractile sheath, the long tail fiber...

journal_title：Nature structural & molecular biology

authors： Kostyuchenko VA,Chipman PR,Leiman PG,Arisaka F,Mesyanzhinov VV,Rossmann MG

更新日期：2005-09-01 00:00:00

abstract：:Many aspects of nucleic acid metabolism, such as DNA replication, repair and transcription, are regulated by the post-translational modifiers ubiquitin and SUMO. Not surprisingly, DNA itself plays an integral part in determining the modification of most chromatin-associated targets. Conversely, ubiquitination or SUMOy...

journal_title：Nature structural & molecular biology

authors： Ulrich HD

更新日期：2014-04-01 00:00:00

abstract：:Although both homologous recombination (HR) and nonhomologous end joining can repair DNA double-strand breaks (DSBs), the mechanisms by which one of these pathways is chosen over the other remain unclear. Here we show that transcriptionally active chromatin is preferentially repaired by HR. Using chromatin immunopreci...

journal_title：Nature structural & molecular biology

authors： Aymard F,Bugler B,Schmidt CK,Guillou E,Caron P,Briois S,Iacovoni JS,Daburon V,Miller KM,Jackson SP,Legube G

更新日期：2014-04-01 00:00:00

abstract：:By sequence-specific binding to 3' UUU-OH, the La protein shields precursor (pre)-RNAs from 3' end digestion and is required to protect defective pre-transfer RNAs from decay. Although La is comprised of a La motif and an RNA-recognition motif (RRM), a recent structure indicates that the RRM beta-sheet surface is not ...

journal_title：Nature structural & molecular biology

authors： Huang Y,Bayfield MA,Intine RV,Maraia RJ

更新日期：2006-07-01 00:00:00

abstract：:Human telomeres are associated with ATM and the protein complex consisting of MRE11, RAD50 and NBS1 (MRN), which are central to maintaining genomic stability. Here we show that when targeted to telomeres, wild-type RAD50 downregulates telomeric association of TRF1, a negative regulator of telomere maintenance. TRF1 bi...

journal_title：Nature structural & molecular biology

authors： Wu Y,Xiao S,Zhu XD

更新日期：2007-09-01 00:00:00

abstract：:Thymic stromal lymphopoietin (TSLP), a cytokine produced by epithelial cells at barrier surfaces, is pivotal for the development of widespread chronic inflammatory disorders such as asthma and atopic dermatitis. The structure of the mouse TSLP-mediated signaling complex reveals how TSLP establishes extensive interface...

journal_title：Nature structural & molecular biology

authors： Verstraete K,van Schie L,Vyncke L,Bloch Y,Tavernier J,Pauwels E,Peelman F,Savvides SN

更新日期：2014-04-01 00:00:00

abstract：:The discovery of the three eukaryotic nuclear RNA polymerases paved the way for serious biochemical investigations of eukaryotic transcription and the identification of eukaryotic transcription factors. Here we describe this adventure from our vantage point, with a focus on the hunt for factors that regulate elongatio...

journal_title：Nature structural & molecular biology

authors： Conaway RC,Conaway JW

更新日期：2019-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Weill L,Belloc E,Castellazzi CL,Méndez R

更新日期：2019-08-01 00:00:00

abstract：:Core Factor (CF) is a conserved RNA polymerase (Pol) I general transcription factor comprising Rrn6, Rrn11 and the TFIIB-related subunit Rrn7. CF binds TATA-binding protein (TBP), Pol I and the regulatory factors Rrn3 and upstream activation factor. We used chemical cross-linking-MS to determine the molecular architec...

journal_title：Nature structural & molecular biology

authors： Knutson BA,Luo J,Ranish J,Hahn S

更新日期：2014-09-01 00:00:00

abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

journal_title：Nature structural & molecular biology

authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

更新日期：2014-05-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused re...

journal_title：Nature structural & molecular biology

authors： Campagne S,Marsh ME,Capitani G,Vorholt JA,Allain FH

更新日期：2014-03-01 00:00:00

abstract：:Induced transcription through CAG repeats in human cells increases repeat contraction approximately 15-fold in both confluent and proliferating cells. Repeats are stabilized against contraction by siRNA knockdown of MSH2, MSH3 or XPA, but not by knockdown of MSH6, XPC or FEN1. These results define a pathway for CAG.CT...

journal_title：Nature structural & molecular biology

authors： Lin Y,Dion V,Wilson JH

更新日期：2006-02-01 00:00:00

abstract：:The human APOBEC3G (A3G) DNA cytosine deaminase restricts and hypermutates DNA-based parasites including HIV-1. The viral infectivity factor (Vif) prevents restriction by triggering A3G degradation. Although the structure of the A3G catalytic domain is known, the structure of the N-terminal Vif-binding domain has prov...

journal_title：Nature structural & molecular biology

authors： Kouno T,Luengas EM,Shigematsu M,Shandilya SM,Zhang J,Chen L,Hara M,Schiffer CA,Harris RS,Matsuo H

更新日期：2015-06-01 00:00:00

abstract：:Tripeptidyl peptidase II (TPP II) is the largest known eukaryotic protease (6 MDa). It is believed to act downstream of the 26S proteasome, cleaving tripeptides from the N termini of longer peptides, and it is implicated in numerous cellular processes. Here we report the structure of Drosophila TPP II determined by a ...

journal_title：Nature structural & molecular biology

authors： Chuang CK,Rockel B,Seyit G,Walian PJ,Schönegge AM,Peters J,Zwart PH,Baumeister W,Jap BK

更新日期：2010-08-01 00:00:00

abstract：:The endosomal sorting complex required for transport (ESCRT) machinery, including ESCRT-III, localizes to the midbody and participates in the membrane-abscission step of cytokinesis. The ESCRT-III protein charged multivesicular body protein 1B (CHMP1B) is required for recruitment of the MIT domain-containing protein s...

journal_title：Nature structural & molecular biology

authors： Yang D,Rismanchi N,Renvoisé B,Lippincott-Schwartz J,Blackstone C,Hurley JH

更新日期：2008-12-01 00:00:00

abstract：:In response to stress, cells attenuate global protein synthesis but permit efficient translation of mRNAs encoding heat-shock proteins (HSPs). Although decades have passed since the first description of the heat-shock response, how cells achieve translational control of HSP synthesis remains enigmatic. Here we report ...

journal_title：Nature structural & molecular biology

authors： Zhang X,Gao X,Coots RA,Conn CS,Liu B,Qian SB

更新日期：2015-05-01 00:00:00

abstract：:The CDK8 kinase module (CKM) is a conserved, dissociable Mediator subcomplex whose component subunits were genetically linked to the RNA polymerase II (RNAPII) C-terminal domain (CTD) and individually recognized as transcriptional repressors before Mediator was identified as a pre-eminent complex in eukaryotic transcr...

journal_title：Nature structural & molecular biology

authors： Tsai KL,Sato S,Tomomori-Sato C,Conaway RC,Conaway JW,Asturias FJ

更新日期：2013-05-01 00:00:00