Structure and mechanism of the RNA polymerase II transcription machinery.

abstract：:Cells respond to changes in environmental conditions via orchestrated modifications in gene expression. For example, in response to heat shock, cells execute a program of gene-specific transcriptional activation and repression. Although the activation of genes upon heat shock has been widely studied, the mechanism of ...

journal_title：Nature structural & molecular biology

authors： Allen TA,Von Kaenel S,Goodrich JA,Kugel JF

更新日期：2004-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Putnam A,Cassani M,Smith J,Seydoux G

更新日期：2020-02-01 00:00:00

abstract：:Induced transcription through CAG repeats in human cells increases repeat contraction approximately 15-fold in both confluent and proliferating cells. Repeats are stabilized against contraction by siRNA knockdown of MSH2, MSH3 or XPA, but not by knockdown of MSH6, XPC or FEN1. These results define a pathway for CAG.CT...

journal_title：Nature structural & molecular biology

authors： Lin Y,Dion V,Wilson JH

更新日期：2006-02-01 00:00:00

abstract：:Histones have two structurally and functionally distinct domains: globular domains forming the nucleosomal core around which DNA is wrapped and unstructured tails protruding from the nucleosomal core. Whereas post-translational modifications (PTMs) in histone tails are well studied, much less is currently known about ...

journal_title：Nature structural & molecular biology

authors： Tropberger P,Schneider R

更新日期：2013-06-01 00:00:00

abstract：:An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from h...

journal_title：Nature structural & molecular biology

authors： Schwartz S,Meshorer E,Ast G

更新日期：2009-09-01 00:00:00

abstract：:Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-ind...

journal_title：Nature structural & molecular biology

authors： Bühler M,Steiner S,Mohn F,Paillusson A,Mühlemann O

更新日期：2006-05-01 00:00:00

abstract：:Chaperones, a group of proteins that assist the folding of other proteins, seem to work in a coordinated manner. Two major chaperone families are heat-shock protein families Hsp60 and Hsp70. Here we show for the first time the formation of a stable complex between chaperonin-containing TCP-1 (CCT) and Hsc70, two eukar...

journal_title：Nature structural & molecular biology

authors： Cuéllar J,Martín-Benito J,Scheres SH,Sousa R,Moro F,López-Viñas E,Gómez-Puertas P,Muga A,Carrascosa JL,Valpuesta JM

更新日期：2008-08-01 00:00:00

abstract：:The CagA protein of Helicobacter pylori interacts with numerous cellular factors and is associated with increased virulence and risk of gastric carcinoma. We present here the cocrystal structure of a subdomain of CagA with the human kinase PAR1b/MARK2, revealing that a CagA peptide mimics substrates of this kinase fam...

journal_title：Nature structural & molecular biology

authors： Nesić D,Miller MC,Quinkert ZT,Stein M,Chait BT,Stebbins CE

更新日期：2010-01-01 00:00:00

abstract：:p53 binds as a tetramer to DNA targets consisting of two decameric half-sites separated by a variable spacer. Here we present high-resolution crystal structures of complexes between p53 core-domain tetramers and DNA targets consisting of contiguous half-sites. In contrast to previously reported p53-DNA complexes that ...

journal_title：Nature structural & molecular biology

authors： Kitayner M,Rozenberg H,Rohs R,Suad O,Rabinovich D,Honig B,Shakked Z

更新日期：2010-04-01 00:00:00

abstract：:Although R loops can occur at different genomic locations, the factors that determine their formation and frequency remain unclear. Emerging evidence indicates that DNA breaks stimulate DNA-RNA hybrid formation. Here, we discuss the possibility that formation of hybrids may be an inevitable risk of DNA breaks that occ...

journal_title：Nature structural & molecular biology

authors： Aguilera A,Gómez-González B

更新日期：2017-05-04 00:00:00

abstract：:Chromatin is a dynamic structure that must respond to myriad stimuli to regulate access to DNA, and chemical modification of histones is a major means by which the cell modulates nucleosome mobility and turnover. Histone modifications are linked to essentially every cellular process requiring DNA access, including tra...

journal_title：Nature structural & molecular biology

authors： Zentner GE,Henikoff S

更新日期：2013-03-01 00:00:00

abstract：:The Ca(2+) sensor synaptotagmin-1 is thought to trigger membrane fusion by binding to acidic membrane lipids and SNARE proteins. Previous work has shown that binding is mediated by electrostatic interactions that are sensitive to the ionic environment. However, the influence of divalent or polyvalent ions, at physiolo...

journal_title：Nature structural & molecular biology

authors： Park Y,Seo JB,Fraind A,Pérez-Lara A,Yavuz H,Han K,Jung SR,Kattan I,Walla PJ,Choi M,Cafiso DS,Koh DS,Jahn R

更新日期：2015-10-01 00:00:00

abstract：:Coupling between transcription and RNA processing is a key gene regulatory mechanism. Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation of the precursor mRNA (pre-mRNA) splicing factors hnRNP A1, U2AF65 and U1 and U5 snRNPs on the intron-containing human FOS gene. These factors w...

journal_title：Nature structural & molecular biology

authors： Listerman I,Sapra AK,Neugebauer KM

更新日期：2006-09-01 00:00:00

abstract：:Human telomeres are associated with ATM and the protein complex consisting of MRE11, RAD50 and NBS1 (MRN), which are central to maintaining genomic stability. Here we show that when targeted to telomeres, wild-type RAD50 downregulates telomeric association of TRF1, a negative regulator of telomere maintenance. TRF1 bi...

journal_title：Nature structural & molecular biology

authors： Wu Y,Xiao S,Zhu XD

更新日期：2007-09-01 00:00:00

abstract：:Mosquito-based malaria transmission-blocking vaccines (mTBVs) target midgut-surface antigens of the Plasmodium parasite's obligate vector, the Anopheles mosquito. The alanyl aminopeptidase N (AnAPN1) is the leading mTBV immunogen; however, AnAPN1's role in Plasmodium infection of the mosquito and how anti-AnAPN1 antib...

journal_title：Nature structural & molecular biology

authors： Atkinson SC,Armistead JS,Mathias DK,Sandeu MM,Tao D,Borhani-Dizaji N,Tarimo BB,Morlais I,Dinglasan RR,Borg NA

更新日期：2015-07-01 00:00:00

abstract：:Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC). In humans, Argonaute2 (AGO2) assembles with the guide RNA-generating enzyme Di...

journal_title：Nature structural & molecular biology

authors： Wang HW,Noland C,Siridechadilok B,Taylor DW,Ma E,Felderer K,Doudna JA,Nogales E

更新日期：2009-11-01 00:00:00

abstract：:The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry ...

journal_title：Nature structural & molecular biology

authors： Park YJ,Walls AC,Wang Z,Sauer MM,Li W,Tortorici MA,Bosch BJ,DiMaio F,Veesler D

更新日期：2019-12-01 00:00:00

abstract：:BAK and BAX are essential mediators of apoptosis that oligomerize in response to death cues, thereby causing permeabilization of the mitochondrial outer membrane. Their transition from quiescent monomers to pore-forming oligomers involves a well-characterized symmetric dimer intermediate. However, no essential seconda...

journal_title：Nature structural & molecular biology

authors： Cowan AD,Smith NA,Sandow JJ,Kapp EA,Rustam YH,Murphy JM,Brouwer JM,Bernardini JP,Roy MJ,Wardak AZ,Tan IK,Webb AI,Gulbis JM,Smith BJ,Reid GE,Dewson G,Colman PM,Czabotar PE

更新日期：2020-11-01 00:00:00

abstract：:Members of the X11/Mint family of multidomain adaptor proteins are composed of a divergent N terminus, a conserved PTB domain and a pair of C-terminal PDZ domains. Many proteins can interact with the PDZ tandem of X11 proteins, although the mechanism of such interactions is unclear. Here we show that the highly conser...

journal_title：Nature structural & molecular biology

authors： Long JF,Feng W,Wang R,Chan LN,Ip FC,Xia J,Ip NY,Zhang M

更新日期：2005-08-01 00:00:00

abstract：:To determine which genomic features promote homologous recombination, we created a genome-wide map of gene targeting sites. We used an adeno-associated virus vector to target identical loci introduced as transcriptionally active retroviral vectors. A comparison of ~2,000 targeted and untargeted sites showed that targe...

journal_title：Nature structural & molecular biology

authors： Deyle DR,Hansen RS,Cornea AM,Li LB,Burt AA,Alexander IE,Sandstrom RS,Stamatoyannopoulos JA,Wei CL,Russell DW

更新日期：2014-11-01 00:00:00

abstract：:Despite growing appreciation of the importance of long noncoding RNAs (lncRNAs) in normal physiology and disease, our knowledge of cancer-related lncRNAs remains limited. By repurposing microarray probes, we constructed expression profiles of 10,207 lncRNA genes in approximately 1,300 tumors over four different cancer...

journal_title：Nature structural & molecular biology

authors： Du Z,Fei T,Verhaak RG,Su Z,Zhang Y,Brown M,Chen Y,Liu XS

更新日期：2013-07-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Weill L,Belloc E,Castellazzi CL,Méndez R

更新日期：2019-08-01 00:00:00

abstract：:The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of n...

journal_title：Nature structural & molecular biology

authors： Quistgaard EM,Madsen P,Grøftehauge MK,Nissen P,Petersen CM,Thirup SS

更新日期：2009-01-01 00:00:00

abstract：:Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discov...

journal_title：Nature structural & molecular biology

authors： Peterson FC,Burgie ES,Park SY,Jensen DR,Weiner JJ,Bingman CA,Chang CE,Cutler SR,Phillips GN Jr,Volkman BF

更新日期：2010-09-01 00:00:00

abstract：:We have determined the solution structure of the complex between an arginine-glycine-rich RGG peptide from the human fragile X mental retardation protein (FMRP) and an in vitro-selected guanine-rich (G-rich) sc1 RNA. The bound RNA forms a newly discovered G-quadruplex separated from the flanking duplex stem by a mixed...

journal_title：Nature structural & molecular biology

authors： Phan AT,Kuryavyi V,Darnell JC,Serganov A,Majumdar A,Ilin S,Raslin T,Polonskaia A,Chen C,Clain D,Darnell RB,Patel DJ

更新日期：2011-06-05 00:00:00

abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

journal_title：Nature structural & molecular biology

authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

更新日期：2014-05-01 00:00:00

abstract：:The proteasome is the main ATP-dependent protease in eukaryotic cells and controls the concentration of many regulatory proteins in the cytosol and nucleus. Proteins are targeted to the proteasome by the covalent attachment of polyubiquitin chains. The ubiquitin modification serves as the proteasome recognition elemen...

journal_title：Nature structural & molecular biology

authors： Prakash S,Tian L,Ratliff KS,Lehotzky RE,Matouschek A

更新日期：2004-09-01 00:00:00

abstract：:Kinases are involved in disease development and modulation of their activity can be therapeutically beneficial. Drug-resistant mutant kinases are valuable tools in drug discovery efforts, but the prediction of mutants across the kinome is challenging. Here, we generate deep mutational scanning data to identify mutant ...

journal_title：Nature structural & molecular biology

authors： Persky NS,Hernandez D,Do Carmo M,Brenan L,Cohen O,Kitajima S,Nayar U,Walker A,Pantel S,Lee Y,Cordova J,Sathappa M,Zhu C,Hayes TK,Ram P,Pancholi P,Mikkelsen TS,Barbie DA,Yang X,Haq R,Piccioni F,Root DE,Johannes

更新日期：2020-01-01 00:00:00

abstract：:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatch...

journal_title：Nature structural & molecular biology

authors： Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

更新日期：2010-07-01 00:00:00

abstract：:Here we report the crystal structures of I.C and I.A wobble base pairs in the context of the ribosomal decoding center, clearly showing that the I.A base pair is of an I(anti).A(anti) conformation, as predicted by Crick. Additionally, the structures enable the observation of changes in the anticodon to allow purine-pu...

journal_title：Nature structural & molecular biology

authors： Murphy FV 4th,Ramakrishnan V

更新日期：2004-12-01 00:00:00