DNA-RNA hybrids: the risks of DNA breakage during transcription.


:Although R loops can occur at different genomic locations, the factors that determine their formation and frequency remain unclear. Emerging evidence indicates that DNA breaks stimulate DNA-RNA hybrid formation. Here, we discuss the possibility that formation of hybrids may be an inevitable risk of DNA breaks that occur within actively transcribed regions. While such hybrids must be removed to permit repair, their potential role as repair intermediates remains to be established.


Nat Struct Mol Biol


Aguilera A,Gómez-González B




Has Abstract


2017-05-04 00:00:00














  • Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.

    abstract::The exon-junction complex (EJC) functionally links splicing to subsequent mRNA localization, translation and stability. Sequence-independent binding of the EJC core to RNA is ensured by the DEAD-box helicase eIF4AIII. Here, we identified the splicing factor CWC22 as a new eIF4AIII partner in flies and humans. CWC22 co...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Barbosa I,Haque N,Fiorini F,Barrandon C,Tomasetto C,Blanchette M,Le Hir H

    更新日期:2012-10-01 00:00:00

  • Structure of a proton-dependent lipid transporter involved in lipoteichoic acids biosynthesis.

    abstract::Lipoteichoic acids (LTAs) are essential cell-wall components in Gram-positive bacteria, including the human pathogen Staphylococcus aureus, contributing to cell adhesion, cell division and antibiotic resistance. Genetic evidence has suggested that LtaA is the flippase that mediates the translocation of the lipid-linke...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Zhang B,Liu X,Lambert E,Mas G,Hiller S,Veening JW,Perez C

    更新日期:2020-06-01 00:00:00

  • Autoinhibition of X11/Mint scaffold proteins revealed by the closed conformation of the PDZ tandem.

    abstract::Members of the X11/Mint family of multidomain adaptor proteins are composed of a divergent N terminus, a conserved PTB domain and a pair of C-terminal PDZ domains. Many proteins can interact with the PDZ tandem of X11 proteins, although the mechanism of such interactions is unclear. Here we show that the highly conser...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Long JF,Feng W,Wang R,Chan LN,Ip FC,Xia J,Ip NY,Zhang M

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  • Regulation of nucleosome dynamics by histone modifications.

    abstract::Chromatin is a dynamic structure that must respond to myriad stimuli to regulate access to DNA, and chemical modification of histones is a major means by which the cell modulates nucleosome mobility and turnover. Histone modifications are linked to essentially every cellular process requiring DNA access, including tra...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章,评审


    authors: Zentner GE,Henikoff S

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Lipp JJ,Marvin MC,Shokat KM,Guthrie C

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  • Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM.

    abstract::The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have been shown to be recruited through the core to reach Rrp44's exo-RNase (EXO) site. Using single-particle EM and biochem...

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  • Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors.

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    journal_title:Nature structural & molecular biology

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    journal_title:Nature structural & molecular biology

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  • Catching DNA with hoops-biophysical approaches to clarify the mechanism of SMC proteins.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章,评审


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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Bradley CM,Ronning DR,Ghirlando R,Craigie R,Dyda F

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  • Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Chowdary TK,Cairns TM,Atanasiu D,Cohen GH,Eisenberg RJ,Heldwein EE

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  • Binding-induced folding of prokaryotic ubiquitin-like protein on the Mycobacterium proteasomal ATPase targets substrates for degradation.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Wang T,Darwin KH,Li H

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  • A popular engagement at the ends.

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    journal_title:Nature structural & molecular biology

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Ghanim GE,Kellogg EH,Nogales E,Rio DC

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    journal_title:Nature structural & molecular biology

    pub_type: 已发布勘误


    authors: Putnam A,Cassani M,Smith J,Seydoux G

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Peterson FC,Burgie ES,Park SY,Jensen DR,Weiner JJ,Bingman CA,Chang CE,Cutler SR,Phillips GN Jr,Volkman BF

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Popovych N,Sun S,Ebright RH,Kalodimos CG

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  • A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Tsai KL,Sato S,Tomomori-Sato C,Conaway RC,Conaway JW,Asturias FJ

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  • The SINE-encoded mouse B2 RNA represses mRNA transcription in response to heat shock.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Allen TA,Von Kaenel S,Goodrich JA,Kugel JF

    更新日期:2004-09-01 00:00:00

  • Molecular basis for H3K36me3 recognition by the Tudor domain of PHF1.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Musselman CA,Avvakumov N,Watanabe R,Abraham CG,Lalonde ME,Hong Z,Allen C,Roy S,Nuñez JK,Nickoloff J,Kulesza CA,Yasui A,Côté J,Kutateladze TG

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  • Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junction.

    abstract::We have determined the solution structure of the complex between an arginine-glycine-rich RGG peptide from the human fragile X mental retardation protein (FMRP) and an in vitro-selected guanine-rich (G-rich) sc1 RNA. The bound RNA forms a newly discovered G-quadruplex separated from the flanking duplex stem by a mixed...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Phan AT,Kuryavyi V,Darnell JC,Serganov A,Majumdar A,Ilin S,Raslin T,Polonskaia A,Chen C,Clain D,Darnell RB,Patel DJ

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  • Structures of the otopetrin proton channels Otop1 and Otop3.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Saotome K,Teng B,Tsui CCA,Lee WH,Tu YH,Kaplan JP,Sansom MSP,Liman ER,Ward AB

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  • MRE11-RAD50-NBS1 and ATM function as co-mediators of TRF1 in telomere length control.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Wu Y,Xiao S,Zhu XD

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  • Notch is a direct negative regulator of the DNA-damage response.

    abstract::The DNA-damage response (DDR) ensures genome stability and proper inheritance of genetic information, both of which are essential to survival. It is presently unclear to what extent other signaling pathways modulate DDR function. Here we show that Notch receptor binds and inactivates ATM kinase and that this mechanism...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Vermezovic J,Adamowicz M,Santarpia L,Rustighi A,Forcato M,Lucano C,Massimiliano L,Costanzo V,Bicciato S,Del Sal G,d'Adda di Fagagna F

    更新日期:2015-05-01 00:00:00

  • Hemifusion arrest by complexin is relieved by Ca2+-synaptotagmin I.

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    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

    更新日期:2006-08-01 00:00:00

  • Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase.

    abstract::Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How these events are coordinated with the cell cycle is poorly understood, particularly in mammalian cells. We report here, based on newly developed techniques for visualizing NPC formation,...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Maeshima K,Iino H,Hihara S,Funakoshi T,Watanabe A,Nishimura M,Nakatomi R,Yahata K,Imamoto F,Hashikawa T,Yokota H,Imamoto N

    更新日期:2010-09-01 00:00:00

  • RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes.

    abstract::We investigated co-transcriptional recruitment of pre-mRNA processing factors to human genes. Capping factors associate with paused RNA polymerase II (pol II) at the 5' ends of quiescent genes. They also track throughout actively transcribed genes and accumulate with paused polymerase in the 3' flanking region. The 3'...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Glover-Cutter K,Kim S,Espinosa J,Bentley DL

    更新日期:2008-01-01 00:00:00

  • Structure of full-length human anti-PD1 therapeutic IgG4 antibody pembrolizumab.

    abstract::Immunoglobulin G4 antibodies exhibit unusual properties with important biological consequences. We report the structure of the human full-length IgG4 S228P anti-PD1 antibody pembrolizumab, solved to 2.3-Å resolution. Pembrolizumab is a compact molecule, consistent with the presence of a short hinge region. The Fc doma...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Scapin G,Yang X,Prosise WW,McCoy M,Reichert P,Johnston JM,Kashi RS,Strickland C

    更新日期:2015-12-01 00:00:00

  • A newly discovered function for RNase L in regulating translation termination.

    abstract::The antiviral and antiproliferative effects of interferons are mediated in part by the 2'-5' oligoadenylate-RNase L RNA decay pathway. RNase L is an endoribonuclease that requires 2'-5' oligoadenylates to cleave single-stranded RNA. In this report we present evidence demonstrating a role for RNase L in translation. We...

    journal_title:Nature structural & molecular biology

    pub_type: 杂志文章


    authors: Le Roy F,Salehzada T,Bisbal C,Dougherty JP,Peltz SW

    更新日期:2005-06-01 00:00:00

  • Author Correction: Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Nature structural & molecular biology

    pub_type: 已发布勘误


    authors: Mahalingan KK,Keith Keenan E,Strickland M,Li Y,Liu Y,Ball HL,Tanner ME,Tjandra N,Roll-Mecak A

    更新日期:2020-09-01 00:00:00