Abstract：
:An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from humans, flies and worms, we found that exons show increased nucleosome-occupancy levels with respect to introns, a finding that we link to differential GC content and nucleosome-disfavoring elements between exons and introns. Analysis of genome-wide chromatin immunoprecipitation data in humans and mice revealed four specific post-translational histone modifications enriched in exons. Our findings indicate that previously described enrichment of H3K36me3 modifications in exons reflects a more fundamental phenomenon, namely increased nucleosome occupancy along exons. Our results suggest an RNA polymerase II-mediated cross-talk between chromatin structure and exon-intron architecture, implying that exon selection may be modulated by chromatin structure.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Schwartz S,Meshorer E,Ast Gdoi
10.1038/nsmb.1659subject
Has Abstractpub_date
2009-09-01 00:00:00pages
990-5issue
9eissn
1545-9993issn
1545-9985pii
nsmb.1659journal_volume
16pub_type
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