Abstract：
:Telomere capping conceals chromosome ends from exonucleases and checkpoints, but the full range of capping mechanisms is not well defined. Telomeres have the potential to form G-quadruplex (G4) DNA, although evidence for telomere G4 DNA function in vivo is limited. In budding yeast, capping requires the Cdc13 protein and is lost at nonpermissive temperatures in cdc13-1 mutants. Here, we use several independent G4 DNA-stabilizing treatments to suppress cdc13-1 capping defects. These include overexpression of three different G4 DNA binding proteins, loss of the G4 DNA unwinding helicase Sgs1, or treatment with small molecule G4 DNA ligands. In vitro, we show that protein-bound G4 DNA at a 3' overhang inhibits 5'→3' resection of a paired strand by exonuclease I. These findings demonstrate that, at least in the absence of full natural capping, G4 DNA can play a positive role at telomeres in vivo.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Smith JS,Chen Q,Yatsunyk LA,Nicoludis JM,Garcia MS,Kranaster R,Balasubramanian S,Monchaud D,Teulade-Fichou MP,Abramowitz L,Schultz DC,Johnson FBdoi
10.1038/nsmb.2033subject
Has Abstractpub_date
2011-04-01 00:00:00pages
478-85issue
4eissn
1545-9993issn
1545-9985pii
nsmb.2033journal_volume
18pub_type
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