USP7 is a SUMO deubiquitinase essential for DNA replication.

abstract：:Simultaneous profiling of transcriptome and chromatin accessibility within single cells is a powerful approach to dissect gene regulatory programs in complex tissues. However, current tools are limited by modest throughput. We now describe an ultra high-throughput method, Paired-seq, for parallel analysis of transcrip...

journal_title：Nature structural & molecular biology

authors： Zhu C,Yu M,Huang H,Juric I,Abnousi A,Hu R,Lucero J,Behrens MM,Hu M,Ren B

更新日期：2019-11-01 00:00:00

abstract：:The histone variant macroH2A1 regulates gene expression important for differentiation, stem-cell reprogramming and tumor suppression. Here, we demonstrate that in primary human cells, macroH2A1 participates in two physically and functionally distinct types of chromatin marked by either H3K27me3 or nine histone acetyla...

journal_title：Nature structural & molecular biology

authors： Chen H,Ruiz PD,Novikov L,Casill AD,Park JW,Gamble MJ

更新日期：2014-11-01 00:00:00

abstract：:We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-...

journal_title：Nature structural & molecular biology

authors： Taggart AJ,DeSimone AM,Shih JS,Filloux ME,Fairbrother WG

更新日期：2012-06-17 00:00:00

abstract：:Integrins are heterodimeric cell-surface adhesion molecules comprising one of 18 possible α-chains and one of eight possible β-chains. They control a range of cell functions in a matrix- and ligand-specific manner. Integrins can be internalized by clathrin-mediated endocytosis (CME) through β subunit-based motifs foun...

journal_title：Nature structural & molecular biology

authors： De Franceschi N,Arjonen A,Elkhatib N,Denessiouk K,Wrobel AG,Wilson TA,Pouwels J,Montagnac G,Owen DJ,Ivaska J

更新日期：2016-02-01 00:00:00

abstract：:Processing of transcribed precursor ribosomal RNA (pre-rRNA) to a mature state is a conserved aspect of ribosome biogenesis in vivo. We developed an affinity-purification system to isolate and analyze in vivo-formed pre-rRNA-containing ribonucleoprotein (RNP) particles (rRNPs) from wild-type E. coli. We observed that ...

journal_title：Nature structural & molecular biology

authors： Gupta N,Culver GM

更新日期：2014-10-01 00:00:00

abstract：:CpG island-like sequences are commonly thought to provide the sole signals for designating constitutively unmethylated regions in the genome, thus generating open chromatin domains within a sea of global repression. Using a new database obtained from comprehensive microarray analysis, we show that unmethylated regions...

journal_title：Nature structural & molecular biology

authors： Straussman R,Nejman D,Roberts D,Steinfeld I,Blum B,Benvenisty N,Simon I,Yakhini Z,Cedar H

更新日期：2009-05-01 00:00:00

abstract：:The human APOBEC3G (A3G) DNA cytosine deaminase restricts and hypermutates DNA-based parasites including HIV-1. The viral infectivity factor (Vif) prevents restriction by triggering A3G degradation. Although the structure of the A3G catalytic domain is known, the structure of the N-terminal Vif-binding domain has prov...

journal_title：Nature structural & molecular biology

authors： Kouno T,Luengas EM,Shigematsu M,Shandilya SM,Zhang J,Chen L,Hara M,Schiffer CA,Harris RS,Matsuo H

更新日期：2015-06-01 00:00:00

abstract：:DNA methylation is an epigenetic modification that is generally associated with repression of transcription initiation at CpG-island promoters. Here we argue that, on the basis of recent high-throughput genomic and proteomic screenings, DNA methylation can also have different outcomes, including activation of transcri...

journal_title：Nature structural & molecular biology

authors： Spruijt CG,Vermeulen M

更新日期：2014-11-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:Structural maintenance of chromosome (SMC) complexes are central regulators of chromosome architecture that are essential in all domains of life. For decades, the structural biology field has been debating how these conserved protein complexes use their intricate ring-like structures to structurally organize DNA. Here...

journal_title：Nature structural & molecular biology

authors： Eeftens J,Dekker C

更新日期：2017-12-07 00:00:00

abstract：:Methylation of histones is central to chromatin regulation, and thus previously unknown mechanisms regulating genome function can be revealed through the discovery of new histone methyl marks. Here we identify Set5 as the first histone H4 methyltransferase, which monomethylates the critical H4 lysine residues 5, 8 and...

journal_title：Nature structural & molecular biology

authors： Green EM,Mas G,Young NL,Garcia BA,Gozani O

更新日期：2012-02-19 00:00:00

abstract：:Although tertiary folding of whole protein domains is prohibited by the cramped dimensions of the ribosomal tunnel, dynamic tertiary interactions may permit folding of small elementary units within the tunnel. To probe this possibility, we used a beta-hairpin and an alpha-helical hairpin from the cytosolic N terminus ...

journal_title：Nature structural & molecular biology

authors： Kosolapov A,Deutsch C

更新日期：2009-04-01 00:00:00

abstract：:Myotonic dystrophy is an RNA gain-of-function disease caused by expanded CUG or CCUG repeats, which sequester the RNA binding protein MBNL1. Here we describe a newly discovered function for MBNL1 as a regulator of pre-miR-1 biogenesis and find that miR-1 processing is altered in heart samples from people with myotonic...

journal_title：Nature structural & molecular biology

authors： Rau F,Freyermuth F,Fugier C,Villemin JP,Fischer MC,Jost B,Dembele D,Gourdon G,Nicole A,Duboc D,Wahbi K,Day JW,Fujimura H,Takahashi MP,Auboeuf D,Dreumont N,Furling D,Charlet-Berguerand N

更新日期：2011-06-19 00:00:00

abstract：:The spliceosome is thought to undergo a conformational change between the two catalytic steps of precursor messenger RNA splicing, although the specific events in this transition are poorly understood. We previously proposed a two-state model of splicing in which the conformations required for the first and second ste...

journal_title：Nature structural & molecular biology

authors： Liu L,Query CC,Konarska MM

更新日期：2007-06-01 00:00:00

abstract：:No-go decay (NGD) targets mRNAs with stalls in translation elongation for endonucleolytic cleavage in a process involving the Dom34 and Hbs1 proteins. The crystal structure of a Schizosaccharomyces pombe Dom34-Hbs1 complex reveals an overall shape similar to that of eRF1-eRF3-GTP and EF-Tu-tRNA-GDPNP. Similarly to eRF...

journal_title：Nature structural & molecular biology

authors： Chen L,Muhlrad D,Hauryliuk V,Cheng Z,Lim MK,Shyp V,Parker R,Song H

更新日期：2010-10-01 00:00:00

abstract：:Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-...

journal_title：Nature structural & molecular biology

authors： Diez M,Zimmermann B,Börsch M,König M,Schweinberger E,Steigmiller S,Reuter R,Felekyan S,Kudryavtsev V,Seidel CA,Gräber P

更新日期：2004-02-01 00:00:00

abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

journal_title：Nature structural & molecular biology

authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

更新日期：2014-05-01 00:00:00

abstract：:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatch...

journal_title：Nature structural & molecular biology

authors： Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

更新日期：2010-07-01 00:00:00

abstract：:The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have been shown to be recruited through the core to reach Rrp44's exo-RNase (EXO) site. Using single-particle EM and biochem...

journal_title：Nature structural & molecular biology

authors： Liu JJ,Bratkowski MA,Liu X,Niu CY,Ke A,Wang HW

更新日期：2014-01-01 00:00:00

abstract：:Allosteric interactions are typically considered to proceed through a series of discrete changes in bonding interactions that alter the protein conformation. Here we show that allostery can be mediated exclusively by transmitted changes in protein motions. We have characterized the negatively cooperative binding of cA...

journal_title：Nature structural & molecular biology

authors： Popovych N,Sun S,Ebright RH,Kalodimos CG

更新日期：2006-09-01 00:00:00

abstract：:Interactions between Src homology 2 (SH2) domains and phosphotyrosine sites regulate tyrosine kinase signaling networks. Selective perturbation of these interactions is challenging due to the high homology among the 120 human SH2 domains. Using an improved phage-display selection system, we generated a small antibody ...

journal_title：Nature structural & molecular biology

authors： Wojcik J,Hantschel O,Grebien F,Kaupe I,Bennett KL,Barkinge J,Jones RB,Koide A,Superti-Furga G,Koide S

更新日期：2010-04-01 00:00:00

abstract：:DNA ligases finalize DNA replication and repair through DNA nick-sealing reactions that can abort to generate cytotoxic 5'-adenylation DNA damage. Aprataxin (Aptx) catalyzes direct reversal of 5'-adenylate adducts to protect genome integrity. Here the structure of a Schizosaccharomyces pombe Aptx-DNA-AMP-Zn(2+) comple...

journal_title：Nature structural & molecular biology

authors： Tumbale P,Appel CD,Kraehenbuehl R,Robertson PD,Williams JS,Krahn J,Ahel I,Williams RS

更新日期：2011-10-09 00:00:00

abstract：: ...

journal_title：Nature structural & molecular biology

authors： Marcinkiewicz KM

更新日期：2018-12-01 00:00:00

abstract：:The N-terminal acetylation of Sir3 is essential for heterochromatin establishment and maintenance in yeast, but its mechanism of action is unknown. The crystal structure of the N-terminally acetylated BAH domain of Saccharomyces cerevisiae Sir3 bound to the nucleosome core particle reveals that the N-terminal acetylat...

journal_title：Nature structural & molecular biology

authors： Arnaudo N,Fernández IS,McLaughlin SH,Peak-Chew SY,Rhodes D,Martino F

更新日期：2013-09-01 00:00:00

abstract：:Core Factor (CF) is a conserved RNA polymerase (Pol) I general transcription factor comprising Rrn6, Rrn11 and the TFIIB-related subunit Rrn7. CF binds TATA-binding protein (TBP), Pol I and the regulatory factors Rrn3 and upstream activation factor. We used chemical cross-linking-MS to determine the molecular architec...

journal_title：Nature structural & molecular biology

authors： Knutson BA,Luo J,Ranish J,Hahn S

更新日期：2014-09-01 00:00:00

abstract：:The Mre11-Rad50-NBS1 (MRN) complex has many roles in response to DNA double-strand breaks, but its functions in repair by nonhomologous end joining (NHEJ) pathways are poorly understood. We have investigated requirements for MRN in class switch recombination (CSR), a programmed DNA rearrangement in B lymphocytes that ...

journal_title：Nature structural & molecular biology

authors： Dinkelmann M,Spehalski E,Stoneham T,Buis J,Wu Y,Sekiguchi JM,Ferguson DO

更新日期：2009-08-01 00:00:00

abstract：:We present a structural analysis of the folding transition states of three SH3 domains. Our results reveal that the secondary structure is not yet fully formed at this stage of folding and that the solvent is only partially excluded from the interior of the protein. Comparison of the members of the transition state en...

journal_title：Nature structural & molecular biology

authors： Lindorff-Larsen K,Vendruscolo M,Paci E,Dobson CM

更新日期：2004-05-01 00:00:00

abstract：:Aggregation of proteins containing polyglutamine (polyQ) expansions characterizes many neurodegenerative disorders, including Huntington's disease. Molecular chaperones modulate the aggregation and toxicity of the huntingtin (Htt) protein by an ill-defined mechanism. Here we determine how the chaperonin TRiC suppresse...

journal_title：Nature structural & molecular biology

authors： Tam S,Spiess C,Auyeung W,Joachimiak L,Chen B,Poirier MA,Frydman J

更新日期：2009-12-01 00:00:00

abstract：:The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation. To distinguish between local displacement versus global unfolding mechanisms for complex disassem...

journal_title：Nature structural & molecular biology

authors： Yang B,Stjepanovic G,Shen Q,Martin A,Hurley JH

更新日期：2015-06-01 00:00:00

abstract：:Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviat...

journal_title：Nature structural & molecular biology

authors： Bange G,Kümmerer N,Grudnik P,Lindner R,Petzold G,Kressler D,Hurt E,Wild K,Sinning I

更新日期：2011-11-06 00:00:00