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Selective integrin endocytosis is driven by interactions between the integrin α-chain and AP2.

Abstract：

:Integrins are heterodimeric cell-surface adhesion molecules comprising one of 18 possible α-chains and one of eight possible β-chains. They control a range of cell functions in a matrix- and ligand-specific manner. Integrins can be internalized by clathrin-mediated endocytosis (CME) through β subunit-based motifs found in all integrin heterodimers. However, whether specific integrin heterodimers can be selectively endocytosed was unknown. Here, we found that a subset of α subunits contain an evolutionarily conserved and functional YxxΦ motif directing integrins to selective internalization by the most abundant endocytic clathrin adaptor, AP2. We determined the structure of the human integrin α4-tail motif in complex with the AP2 C-μ2 subunit and confirmed the interaction by isothermal titration calorimetry. Mutagenesis of the motif impaired selective heterodimer endocytosis and attenuated integrin-mediated cell migration. We propose that integrins evolved to enable selective integrin-receptor turnover in response to changing matrix conditions.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

De Franceschi N,Arjonen A,Elkhatib N,Denessiouk K,Wrobel AG,Wilson TA,Pouwels J,Montagnac G,Owen DJ,Ivaska J

doi

10.1038/nsmb.3161

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

172-9

issue

2

eissn

1545-9993

issn

1545-9985

pii

nsmb.3161

journal_volume

23

pub_type

杂志文章

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