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Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA.

Abstract：

:In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA. Using purified components in the presence of ATP-γS, we have captured in vitro an intermediate in pre-RC assembly that contains a complex between the ORC-Cdc6 and Cdt1-MCM2-7 heteroheptamers called the OCCM. Cryo-EM studies of this 14-subunit complex reveal that the two separate heptameric complexes are engaged extensively, with the ORC-Cdc6 N-terminal AAA+ domains latching onto the C-terminal AAA+ motor domains of the MCM2-7 hexamer. The conformation of ORC-Cdc6 undergoes a concerted change into a right-handed spiral with helical symmetry that is identical to that of the DNA double helix. The resulting ORC-Cdc6 helicase loader shows a notable structural similarity to the replication factor C clamp loader, suggesting a conserved mechanism of action.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Sun J,Evrin C,Samel SA,Fernández-Cid A,Riera A,Kawakami H,Stillman B,Speck C,Li H

doi

10.1038/nsmb.2629

subject

Has Abstract

pub_date

2013-08-01 00:00:00

pages

944-51

issue

8

eissn

1545-9993

issn

1545-9985

pii

nsmb.2629

journal_volume

20

pub_type

杂志文章

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