Abstract：
:During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1 and C2. Both intermediates contain Rad51, but whereas the donor duplex remains intact in C1, the ssDNA strand is intertwined with the complementary strand of the donor duplex in C2. Swi5-Sfr1, an evolutionarily conserved recombination activator, facilitates the C1-C2 transition and subsequent ssDNA release from C2 to complete strand exchange in an ATP-hydrolysis-dependent manner. In contrast, Ca2+, which activates the Rad51 filament by curbing ATP hydrolysis, facilitates the C1-C2 transition but does not promote strand exchange. These results reveal that Swi5-Sfr1 and Ca2+ have different activation modes in the late synaptic phase, despite their common function in stabilizing the presynaptic filament.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Ito K,Murayama Y,Takahashi M,Iwasaki Hdoi
10.1038/s41594-017-0002-8subject
Has Abstractpub_date
2018-01-01 00:00:00pages
29-36issue
1eissn
1545-9993issn
1545-9985pii
10.1038/s41594-017-0002-8journal_volume
25pub_type
杂志文章abstract：:Neuronal communication is mediated by Ca(2+)-triggered fusion of transmitter-filled synaptic vesicles with the presynaptic plasma membrane. Synaptotagmin I functions as a Ca(2+) sensor that regulates exocytosis, whereas soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins in th...
journal_title：Nature structural & molecular biology
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journal_title：Nature structural & molecular biology
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更新日期：2014-11-01 00:00:00
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journal_title：Nature structural & molecular biology
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更新日期：2016-06-01 00:00:00
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journal_title：Nature structural & molecular biology
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更新日期：2006-10-01 00:00:00
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journal_title：Nature structural & molecular biology
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更新日期：2004-09-01 00:00:00
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journal_title：Nature structural & molecular biology
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更新日期：2017-12-01 00:00:00
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更新日期：2017-04-01 00:00:00
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更新日期：2009-01-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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更新日期：2020-08-01 00:00:00
