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Polypyrimidine tract binding protein controls the transition from exon definition to an intron defined spliceosome.

Abstract：

:The polypyrimidine tract binding protein (PTB) binds pre-mRNAs to alter splice-site choice. We characterized a series of spliceosomal complexes that assemble on a pre-mRNA under conditions of either PTB-mediated splicing repression or its absence. In the absence of repression, exon definition complexes that were assembled downstream of the regulated exon could progress to pre-spliceosomal A complexes and functional spliceosomes. Under PTB-mediated repression, assembly was arrested at an A-like complex that was unable to transition to spliceosomal complexes. Trans-splicing experiments indicated that, even when the U1 and U2 small nuclear ribonucleoprotein particles (snRNPs) are properly bound to the upstream and downstream exons, the presence of PTB prevents the interaction of the two exon complexes. Proteomic analyses of these complexes provide a new description of exon definition complexes, and indicate that splicing regulators can act on the transition between the exon definition complex and an intron-defined spliceosome.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Sharma S,Kohlstaedt LA,Damianov A,Rio DC,Black DL

doi

10.1038/nsmb.1375

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

183-91

issue

2

eissn

1545-9993

issn

1545-9985

pii

nsmb.1375

journal_volume

15

pub_type

杂志文章

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