Structural basis for protein-RNA recognition in telomerase.

abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

journal_title：Nature structural & molecular biology

authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

更新日期：2014-05-01 00:00:00

abstract：:A class of riboswitches that recognizes guanine and discriminates against other purine analogs was recently identified. RNAs that carry the consensus sequence and structural features of guanine riboswitches are located in the 5' untranslated region (UTR) of numerous prokaryotic genes, where they control the expression...

journal_title：Nature structural & molecular biology

authors： Mandal M,Breaker RR

更新日期：2004-01-01 00:00:00

abstract：:Post-translational modification of proteins by ubiquitin (Ub) and Ub-like modifiers regulates DNA replication. We have previously shown that chromatin around replisomes is rich in SUMO and poor in Ub, whereas mature chromatin exhibits an opposite pattern. How this SUMO-rich, Ub-poor environment is maintained at sites ...

journal_title：Nature structural & molecular biology

authors： Lecona E,Rodriguez-Acebes S,Specks J,Lopez-Contreras AJ,Ruppen I,Murga M,Muñoz J,Mendez J,Fernandez-Capetillo O

更新日期：2016-04-01 00:00:00

abstract：:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that a...

journal_title：Nature structural & molecular biology

authors： Takeshita D,Tomita K

更新日期：2012-01-15 00:00:00

abstract：:Immunoglobulin G4 antibodies exhibit unusual properties with important biological consequences. We report the structure of the human full-length IgG4 S228P anti-PD1 antibody pembrolizumab, solved to 2.3-Å resolution. Pembrolizumab is a compact molecule, consistent with the presence of a short hinge region. The Fc doma...

journal_title：Nature structural & molecular biology

authors： Scapin G,Yang X,Prosise WW,McCoy M,Reichert P,Johnston JM,Kashi RS,Strickland C

更新日期：2015-12-01 00:00:00

abstract：:Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How these events are coordinated with the cell cycle is poorly understood, particularly in mammalian cells. We report here, based on newly developed techniques for visualizing NPC formation,...

journal_title：Nature structural & molecular biology

authors： Maeshima K,Iino H,Hihara S,Funakoshi T,Watanabe A,Nishimura M,Nakatomi R,Yahata K,Imamoto F,Hashikawa T,Yokota H,Imamoto N

更新日期：2010-09-01 00:00:00

abstract：:The long noncoding RNA Xist is expressed from only the paternal X chromosome in mouse preimplantation female embryos and mediates transcriptional silencing of that chromosome. In females, absence of Xist leads to postimplantation lethality. Here, through single-cell RNA sequencing of early preimplantation mouse embryo...

journal_title：Nature structural & molecular biology

authors： Borensztein M,Syx L,Ancelin K,Diabangouaya P,Picard C,Liu T,Liang JB,Vassilev I,Galupa R,Servant N,Barillot E,Surani A,Chen CJ,Heard E

更新日期：2017-03-01 00:00:00

abstract：:In eukaryotes, the essential dimeric molecular chaperone Hsp90 is required for the activation and maturation of specific substrates such as steroid hormone receptors, tyrosine kinases and transcription factors. Hsp90 is involved in the establishment of cancer and has become an attractive target for drug design. Here w...

journal_title：Nature structural & molecular biology

authors： Hagn F,Lagleder S,Retzlaff M,Rohrberg J,Demmer O,Richter K,Buchner J,Kessler H

更新日期：2011-09-04 00:00:00

abstract：:Proteins regulate gene expression by controlling mRNA biogenesis, localization, translation and decay. Identifying the composition, diversity and function of mRNA-protein complexes (mRNPs) is essential to understanding these processes. In a global survey of Saccharomyces cerevisiae mRNA-binding proteins, we identified...

journal_title：Nature structural & molecular biology

authors： Mitchell SF,Jain S,She M,Parker R

更新日期：2013-01-01 00:00:00

abstract：:Polycomb repressive complex 2 (PRC2) catalyzes methylation on lysine 27 of histone H3 (H3K27) and is required for maintaining transcriptional patterns and cellular identity, but the specification and maintenance of genomic PRC2 binding and H3K27 methylation patterns remain incompletely understood. Epigenetic mechanism...

journal_title：Nature structural & molecular biology

authors： Højfeldt JW,Laugesen A,Willumsen BM,Damhofer H,Hedehus L,Tvardovskiy A,Mohammad F,Jensen ON,Helin K

更新日期：2018-03-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Mahalingan KK,Keith Keenan E,Strickland M,Li Y,Liu Y,Ball HL,Tanner ME,Tjandra N,Roll-Mecak A

更新日期：2020-09-01 00:00:00

abstract：:Myosin V is a double-headed processive molecular motor that moves along an actin filament by taking 36-nm steps. Using optical trapping nanometry with high spatiotemporal resolution, we discovered that there are two possible pathways for the 36-nm steps, one with 12- and 24-nm substeps, in this order, and the other wi...

journal_title：Nature structural & molecular biology

authors： Uemura S,Higuchi H,Olivares AO,De La Cruz EM,Ishiwata S

更新日期：2004-09-01 00:00:00

abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

journal_title：Nature structural & molecular biology

authors： Vincent JA,Kwong TJ,Tsukiyama T

更新日期：2008-05-01 00:00:00

abstract：:Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of known 3D structures for these enzymes, their mechanism of action has remained poorly understood. Here we report the X-...

journal_title：Nature structural & molecular biology

authors： Zehr E,Szyk A,Piszczek G,Szczesna E,Zuo X,Roll-Mecak A

更新日期：2017-09-01 00:00:00

abstract：:Ribosomes catalyze the formation of peptide bonds between aminoacyl esters of transfer RNAs within a catalytic center composed of ribosomal RNA only. Here we show that the reaction of P-site formylmethionine (fMet)-tRNA(fMet) with a modified A-site tRNA substrate, Phelac-tRNA(Phe), in which the nucleophilic amino grou...

journal_title：Nature structural & molecular biology

authors： Bieling P,Beringer M,Adio S,Rodnina MV

更新日期：2006-05-01 00:00:00

abstract：:Green tea has held a long-standing place in traditional Asian medicine. Scientific research is now beginning to explain why. ...

journal_title：Nature structural & molecular biology

authors：

更新日期：2008-06-01 00:00:00

abstract：:A fundamental question is how enzymes can accelerate chemical reactions. Catalysis is not only defined by actual chemical steps, but also by enzyme structure and dynamics. To investigate the role of protein dynamics in enzymatic turnover, we measured residue-specific protein dynamics in hyperthermophilic and mesophili...

journal_title：Nature structural & molecular biology

authors： Wolf-Watz M,Thai V,Henzler-Wildman K,Hadjipavlou G,Eisenmesser EZ,Kern D

更新日期：2004-10-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Fichou Y,Han S

更新日期：2019-06-01 00:00:00

abstract：:Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-...

journal_title：Nature structural & molecular biology

authors： Diez M,Zimmermann B,Börsch M,König M,Schweinberger E,Steigmiller S,Reuter R,Felekyan S,Kudryavtsev V,Seidel CA,Gräber P

更新日期：2004-02-01 00:00:00

abstract：:Dicer has a central role in RNA-interference pathways by cleaving double-stranded RNAs (dsRNAs) to produce small regulatory RNAs. Human Dicer can process long double-stranded and hairpin precursor RNAs to yield short interfering RNAs (siRNAs) and microRNAs (miRNAs), respectively. Previous studies have shown that pre-m...

journal_title：Nature structural & molecular biology

authors： Taylor DW,Ma E,Shigematsu H,Cianfrocco MA,Noland CL,Nagayama K,Nogales E,Doudna JA,Wang HW

更新日期：2013-06-01 00:00:00

abstract：:DNA ligases finalize DNA replication and repair through DNA nick-sealing reactions that can abort to generate cytotoxic 5'-adenylation DNA damage. Aprataxin (Aptx) catalyzes direct reversal of 5'-adenylate adducts to protect genome integrity. Here the structure of a Schizosaccharomyces pombe Aptx-DNA-AMP-Zn(2+) comple...

journal_title：Nature structural & molecular biology

authors： Tumbale P,Appel CD,Kraehenbuehl R,Robertson PD,Williams JS,Krahn J,Ahel I,Williams RS

更新日期：2011-10-09 00:00:00

abstract：:Maintenance of cell volume against osmotic change is crucial for proper cell functions. Leucine-rich repeat-containing 8 proteins are anion-selective channels that extrude anions to decrease the cell volume on cellular swelling. Here, we present the structure of human leucine-rich repeat-containing 8A, determined by s...

journal_title：Nature structural & molecular biology

authors： Kasuya G,Nakane T,Yokoyama T,Jia Y,Inoue M,Watanabe K,Nakamura R,Nishizawa T,Kusakizako T,Tsutsumi A,Yanagisawa H,Dohmae N,Hattori M,Ichijo H,Yan Z,Kikkawa M,Shirouzu M,Ishitani R,Nureki O

更新日期：2018-09-01 00:00:00

abstract：:The potassium channel selectivity filter both discriminates between K(+) and sodium ions and contributes to gating of ion flow. Static structures of conducting (open) and nonconducting (inactivated) conformations of this filter are known; however, the sequence of protein rearrangements that connect these two states is...

journal_title：Nature structural & molecular biology

authors： Wang DT,Hill AP,Mann SA,Tan PS,Vandenberg JI

更新日期：2011-01-01 00:00:00

abstract：:The highly contagious measles virus infects millions of individuals worldwide, causing serious disease in children of developing countries. Infection is initiated by attachment of the measles virus hemagglutinin (MV-H), a glycoprotein anchored to the virus envelope, to the host cell receptors CD46 or signaling lymphoc...

journal_title：Nature structural & molecular biology

authors： Santiago C,Celma ML,Stehle T,Casasnovas JM

更新日期：2010-01-01 00:00:00

abstract：:During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1...

journal_title：Nature structural & molecular biology

authors： Ito K,Murayama Y,Takahashi M,Iwasaki H

更新日期：2018-01-01 00:00:00

abstract：:Small-molecule BET inhibitors interfere with the epigenetic interactions between acetylated histones and the bromodomains of the BET family proteins, including BRD4, and they potently inhibit growth of malignant cells by targeting cancer-promoting genes. BRD4 interacts with the pause-release factor P-TEFb and has been...

journal_title：Nature structural & molecular biology

authors： Kanno T,Kanno Y,LeRoy G,Campos E,Sun HW,Brooks SR,Vahedi G,Heightman TD,Garcia BA,Reinberg D,Siebenlist U,O'Shea JJ,Ozato K

更新日期：2014-12-01 00:00:00

abstract：:Telomeres may regulate human disease by at least two independent mechanisms. First, replicative senescence occurs once short telomeres generate DNA-damage signals that produce a barrier to tumor progression. Second, telomere position effects (TPE) could change gene expression at intermediate telomere lengths in cultur...

journal_title：Nature structural & molecular biology

authors： Stadler G,Rahimov F,King OD,Chen JC,Robin JD,Wagner KR,Shay JW,Emerson CP Jr,Wright WE

更新日期：2013-06-01 00:00:00

abstract：:Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryot...

journal_title：Nature structural & molecular biology

authors： Losey HC,Ruthenburg AJ,Verdine GL

更新日期：2006-02-01 00:00:00

abstract：:The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positions. ISWI regulates nucleosome positioning by requiring a minimal length of extranucleosomal DNA for moving nucleosomes. ISW2 from Saccharomyces cerevisiae, a member of the ISWI family, has a conserved domain cal...

journal_title：Nature structural & molecular biology

authors： Hota SK,Bhardwaj SK,Deindl S,Lin YC,Zhuang X,Bartholomew B

更新日期：2013-02-01 00:00:00

abstract：:The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have been shown to be recruited through the core to reach Rrp44's exo-RNase (EXO) site. Using single-particle EM and biochem...

journal_title：Nature structural & molecular biology

authors： Liu JJ,Bratkowski MA,Liu X,Niu CY,Ke A,Wang HW

更新日期：2014-01-01 00:00:00