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Molecular basis for RNA polymerization by Qβ replicase.

Abstract：

:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that at the initiation stage, the 3'-adenine of the template RNA provides a stable platform for de novo initiation. EF-Tu in Qβ replicase forms a template exit channel with the β-subunit. At the elongation stages, the C-terminal region of the β-subunit, assisted by EF-Tu, splits the temporarily double-stranded RNA between the template and nascent RNAs before translocation of the single-stranded template RNA into the exit channel. Therefore, EF-Tu in Qβ replicase modulates RNA elongation processes in a distinct manner from its established function in protein synthesis.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Takeshita D,Tomita K

doi

10.1038/nsmb.2204

subject

Has Abstract

pub_date

2012-01-15 00:00:00

pages

229-37

issue

2

eissn

1545-9993

issn

1545-9985

pii

nsmb.2204

journal_volume

19

pub_type

杂志文章

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