The N-terminal acetylation of Sir3 stabilizes its binding to the nucleosome core particle.

abstract：:The discovery of the three eukaryotic nuclear RNA polymerases paved the way for serious biochemical investigations of eukaryotic transcription and the identification of eukaryotic transcription factors. Here we describe this adventure from our vantage point, with a focus on the hunt for factors that regulate elongatio...

journal_title：Nature structural & molecular biology

authors： Conaway RC,Conaway JW

更新日期：2019-09-01 00:00:00

abstract：:In this article, the Ponceau staining presented in Fig. 1b (right, bottom) does not follow best practices for figure preparation since itinadvertently included duplications from the Ponceau staining presented in Supplementary Fig. 1b (for which the same preparation ofnucleosomes from HeLa cells had been used). A new F...

journal_title：Nature structural & molecular biology

authors： Daujat S,Weiss T,Mohn F,Lange UC,Ziegler-Birling C,Zeissler U,Lappe M,Schübeler D,Torres-Padilla ME,Schneider R

更新日期：2018-08-01 00:00:00

abstract：:Chaperones, a group of proteins that assist the folding of other proteins, seem to work in a coordinated manner. Two major chaperone families are heat-shock protein families Hsp60 and Hsp70. Here we show for the first time the formation of a stable complex between chaperonin-containing TCP-1 (CCT) and Hsc70, two eukar...

journal_title：Nature structural & molecular biology

authors： Cuéllar J,Martín-Benito J,Scheres SH,Sousa R,Moro F,López-Viñas E,Gómez-Puertas P,Muga A,Carrascosa JL,Valpuesta JM

更新日期：2008-08-01 00:00:00

abstract：:Binding of the gp120 envelope (Env) glycoprotein to the CD4 receptor is the first step in the HIV-1 infectious cycle. Although the CD4-binding site has been extensively characterized, the initial receptor interaction has been difficult to study because of major CD4-induced structural rearrangements. Here we used cryog...

journal_title：Nature structural & molecular biology

authors： Liu Q,Acharya P,Dolan MA,Zhang P,Guzzo C,Lu J,Kwon A,Gururani D,Miao H,Bylund T,Chuang GY,Druz A,Zhou T,Rice WJ,Wigge C,Carragher B,Potter CS,Kwong PD,Lusso P

更新日期：2017-04-01 00:00:00

abstract：:We describe a novel approach to separate two ribosome populations from the same cells and use this method in combination with RNA-seq to identify mRNAs bound to Saccharomyces cerevisiae ribosomes with and without Rps26, a protein linked to the pathogenesis of Diamond-Blackfan anemia (DBA). These analyses reveal that R...

journal_title：Nature structural & molecular biology

authors： Ferretti MB,Ghalei H,Ward EA,Potts EL,Karbstein K

更新日期：2017-09-01 00:00:00

abstract：:Mosquito-based malaria transmission-blocking vaccines (mTBVs) target midgut-surface antigens of the Plasmodium parasite's obligate vector, the Anopheles mosquito. The alanyl aminopeptidase N (AnAPN1) is the leading mTBV immunogen; however, AnAPN1's role in Plasmodium infection of the mosquito and how anti-AnAPN1 antib...

journal_title：Nature structural & molecular biology

authors： Atkinson SC,Armistead JS,Mathias DK,Sandeu MM,Tao D,Borhani-Dizaji N,Tarimo BB,Morlais I,Dinglasan RR,Borg NA

更新日期：2015-07-01 00:00:00

abstract：:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that a...

journal_title：Nature structural & molecular biology

authors： Takeshita D,Tomita K

更新日期：2012-01-15 00:00:00

abstract：:Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal struc...

journal_title：Nature structural & molecular biology

authors： Huang J,Brown AF,Wu J,Xue J,Bley CJ,Rand DP,Wu L,Zhang R,Chen JJ,Lei M

更新日期：2014-06-01 00:00:00

abstract：:We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-...

journal_title：Nature structural & molecular biology

authors： Taggart AJ,DeSimone AM,Shih JS,Filloux ME,Fairbrother WG

更新日期：2012-06-17 00:00:00

abstract：:The PHD finger protein 1 (PHF1) is essential in epigenetic regulation and genome maintenance. Here we show that the Tudor domain of human PHF1 binds to histone H3 trimethylated at Lys36 (H3K36me3). We report a 1.9-Å resolution crystal structure of the Tudor domain in complex with H3K36me3 and describe the molecular me...

journal_title：Nature structural & molecular biology

authors： Musselman CA,Avvakumov N,Watanabe R,Abraham CG,Lalonde ME,Hong Z,Allen C,Roy S,Nuñez JK,Nickoloff J,Kulesza CA,Yasui A,Côté J,Kutateladze TG

更新日期：2012-12-01 00:00:00

abstract：:The exosome is an RNA-decay complex that constantly monitors transcription and contributes to post-transcriptional turnover of faulty mRNAs. Yet how nuclear RNA surveillance by the exosome is coordinated with transcription is still unknown. Here we show that the RNA exosome of Schizosaccharomyces pombe can target the ...

journal_title：Nature structural & molecular biology

authors： Lemay JF,Larochelle M,Marguerat S,Atkinson S,Bähler J,Bachand F

更新日期：2014-10-01 00:00:00

abstract：:The threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower-respiratory-tract infections requiring hospitalization. We report here the 3.4-Å-resolution cryo-EM reconstruction of the HCoV-NL6...

journal_title：Nature structural & molecular biology

authors： Walls AC,Tortorici MA,Frenz B,Snijder J,Li W,Rey FA,DiMaio F,Bosch BJ,Veesler D

更新日期：2016-10-01 00:00:00

abstract：:The DNA-damage response (DDR) ensures genome stability and proper inheritance of genetic information, both of which are essential to survival. It is presently unclear to what extent other signaling pathways modulate DDR function. Here we show that Notch receptor binds and inactivates ATM kinase and that this mechanism...

journal_title：Nature structural & molecular biology

authors： Vermezovic J,Adamowicz M,Santarpia L,Rustighi A,Forcato M,Lucano C,Massimiliano L,Costanzo V,Bicciato S,Del Sal G,d'Adda di Fagagna F

更新日期：2015-05-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression, including type I interferon production, by promoting host mRNA degradation and inhibiting host translation, in infected cells. We present evidence that nsp1 uses a novel, two-pronged strategy to inhibit host translation and gen...

journal_title：Nature structural & molecular biology

authors： Kamitani W,Huang C,Narayanan K,Lokugamage KG,Makino S

更新日期：2009-11-01 00:00:00

abstract：:Mg-ADP release is considered to be a crucial process for the regulation and motility of kinesin. To gain insight into the structural basis of this process, we solved the atomic structures of kinesin superfamily protein-1A (KIF1A) during and after Mg(2+) release. On the basis of new structural and mutagenesis data, we ...

journal_title：Nature structural & molecular biology

authors： Nitta R,Okada Y,Hirokawa N

更新日期：2008-10-01 00:00:00

abstract：:Polycomb repressive complex 2 (PRC2) catalyzes methylation on lysine 27 of histone H3 (H3K27) and is required for maintaining transcriptional patterns and cellular identity, but the specification and maintenance of genomic PRC2 binding and H3K27 methylation patterns remain incompletely understood. Epigenetic mechanism...

journal_title：Nature structural & molecular biology

authors： Højfeldt JW,Laugesen A,Willumsen BM,Damhofer H,Hedehus L,Tvardovskiy A,Mohammad F,Jensen ON,Helin K

更新日期：2018-03-01 00:00:00

abstract：:Multiple Argonaute proteins are implicated in gene silencing by RNA interference (RNAi), but only one is known to be an endonuclease that can cleave target mRNAs. Chimeric Argonaute proteins now reveal an unexpected mechanism by which mutations distal to the catalytic center can unmask intrinsic catalytic activity, re...

journal_title：Nature structural & molecular biology

authors： Kidwell MA,Doudna JA

更新日期：2013-07-01 00:00:00

abstract：:The asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a) acts as a repressive mark that antagonizes trimethylation of H3 lysine 4. Here we report that H3R2 is also symmetrically dimethylated (H3R2me2s) by PRMT5 and PRMT7 and present in euchromatic regions. Profiling of H3-tail interactors by SILAC MS revealed ...

journal_title：Nature structural & molecular biology

authors： Migliori V,Müller J,Phalke S,Low D,Bezzi M,Mok WC,Sahu SK,Gunaratne J,Capasso P,Bassi C,Cecatiello V,De Marco A,Blackstock W,Kuznetsov V,Amati B,Mapelli M,Guccione E

更新日期：2012-01-08 00:00:00

abstract：:Phosphorylation of the spliceosome is essential for RNA splicing, yet how and to what extent kinase signaling affects splicing have not been defined on a genome-wide basis. Using a chemical genetic approach, we show in Schizosaccharomyces pombe that the SR protein kinase Dsk1 is required for efficient splicing of intr...

journal_title：Nature structural & molecular biology

authors： Lipp JJ,Marvin MC,Shokat KM,Guthrie C

更新日期：2015-08-01 00:00:00

abstract：:The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its ...

journal_title：Nature structural & molecular biology

authors： Yamada T,Yoshida T,Kawamoto A,Mitsuoka K,Iwasaki K,Tsuge H

更新日期：2020-03-01 00:00:00

abstract：:Herpesviruses, which cause many incurable diseases, infect cells by fusing viral and cellular membranes. Whereas most other enveloped viruses use a single viral catalyst called a fusogen, herpesviruses, inexplicably, require two conserved fusion-machinery components, gB and the heterodimer gH-gL, plus other nonconserv...

journal_title：Nature structural & molecular biology

authors： Chowdary TK,Cairns TM,Atanasiu D,Cohen GH,Eisenberg RJ,Heldwein EE

更新日期：2010-07-01 00:00:00

abstract：:p53 binds as a tetramer to DNA targets consisting of two decameric half-sites separated by a variable spacer. Here we present high-resolution crystal structures of complexes between p53 core-domain tetramers and DNA targets consisting of contiguous half-sites. In contrast to previously reported p53-DNA complexes that ...

journal_title：Nature structural & molecular biology

authors： Kitayner M,Rozenberg H,Rohs R,Suad O,Rabinovich D,Honig B,Shakked Z

更新日期：2010-04-01 00:00:00

abstract：:The core of the neurotransmitter release machinery is formed by SNARE complexes, which bring the vesicle and plasma membranes together and are key for fusion, and by Munc18-1, which controls SNARE-complex formation and may also have a direct role in fusion. In addition, SNARE complex assembly is likely orchestrated by...

journal_title：Nature structural & molecular biology

authors： Rizo J,Rosenmund C

更新日期：2008-07-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Putnam A,Cassani M,Smith J,Seydoux G

更新日期：2020-02-01 00:00:00

abstract：:The spliceosome is thought to undergo a conformational change between the two catalytic steps of precursor messenger RNA splicing, although the specific events in this transition are poorly understood. We previously proposed a two-state model of splicing in which the conformations required for the first and second ste...

journal_title：Nature structural & molecular biology

authors： Liu L,Query CC,Konarska MM

更新日期：2007-06-01 00:00:00

abstract：:Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification ...

journal_title：Nature structural & molecular biology

authors： Sellers JR,Veigel C

更新日期：2010-05-01 00:00:00

abstract：:Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviat...

journal_title：Nature structural & molecular biology

authors： Bange G,Kümmerer N,Grudnik P,Lindner R,Petzold G,Kressler D,Hurt E,Wild K,Sinning I

更新日期：2011-11-06 00:00:00

abstract：:The typical function of kinesins is to transport cargo along microtubules. Binding of ATP to microtubule-attached motile kinesins leads to cargo displacement. To better understand the nature of the conformational changes that lead to the power stroke that moves a kinesin's load along a microtubule, we determined the X...

journal_title：Nature structural & molecular biology

authors： Gigant B,Wang W,Dreier B,Jiang Q,Pecqueur L,Plückthun A,Wang C,Knossow M

更新日期：2013-08-01 00:00:00

abstract：:Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discov...

journal_title：Nature structural & molecular biology

authors： Peterson FC,Burgie ES,Park SY,Jensen DR,Weiner JJ,Bingman CA,Chang CE,Cutler SR,Phillips GN Jr,Volkman BF

更新日期：2010-09-01 00:00:00

abstract：:The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of n...

journal_title：Nature structural & molecular biology

authors： Quistgaard EM,Madsen P,Grøftehauge MK,Nissen P,Petersen CM,Thirup SS

更新日期：2009-01-01 00:00:00