Abstract：
:The threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower-respiratory-tract infections requiring hospitalization. We report here the 3.4-Å-resolution cryo-EM reconstruction of the HCoV-NL63 coronavirus spike glycoprotein trimer, which mediates entry into host cells and is the main target of neutralizing antibodies during infection. The map resolves the extensive glycan shield obstructing the protein surface and, in combination with mass spectrometry, provides a structural framework to understand the accessibility to antibodies. The structure reveals the complete architecture of the fusion machinery including the triggering loop and the C-terminal domains, which contribute to anchoring the trimer to the viral membrane. Our data further suggest that HCoV-NL63 and other coronaviruses use molecular trickery, based on epitope masking with glycans and activating conformational changes, to evade the immune system of infected hosts.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Walls AC,Tortorici MA,Frenz B,Snijder J,Li W,Rey FA,DiMaio F,Bosch BJ,Veesler Ddoi
10.1038/nsmb.3293subject
Has Abstractpub_date
2016-10-01 00:00:00pages
899-905issue
10eissn
1545-9993issn
1545-9985pii
nsmb.3293journal_volume
23pub_type
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journal_title：Nature structural & molecular biology
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journal_title：Nature structural & molecular biology
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