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Crystal structure of the ribosome recycling factor bound to the ribosome.

Abstract：

:In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a stop codon in the A site, a transfer RNA anticodon stem-loop in the P site and tRNA(fMet) in the E site. The work demonstrates that structures of translation factors bound to 70S ribosomes can be determined at reasonably high resolution. Contrary to earlier reports, we did not observe any RRF-induced changes in bridges connecting the two subunits. This suggests that such changes are not a direct requirement for or consequence of RRF binding but possibly arise from the subsequent stabilization of a hybrid state of the ribosome.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Weixlbaumer A,Petry S,Dunham CM,Selmer M,Kelley AC,Ramakrishnan V

doi

10.1038/nsmb1282

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

733-7

issue

8

eissn

1545-9993

issn

1545-9985

pii

nsmb1282

journal_volume

14

pub_type

杂志文章

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