Abstract:
:Agents that activate expression of specific genes to probe cellular pathways or alleviate disease would go beyond existing approaches for controlling gene expression. Duplex RNAs complementary to promoter regions can repress or activate gene expression. The mechanism of these promoter-directed antigene RNAs (agRNAs) has been obscure. Other work has revealed noncoding transcripts that overlap mRNAs. The function of these noncoding transcripts is also not understood. Here we link these two sets of enigmatic results. We find that antisense transcripts are the target for agRNAs that activate or repress expression of progesterone receptor (PR). agRNAs recruit Argonaute proteins to PR antisense transcripts and shift localization of the heterogeneous nuclear ribonucleoprotein-k, RNA polymerase II and heterochromatin protein 1 gamma. Our data demonstrate that antisense transcripts have a central role in recognition of the PR promoter by both activating and inhibitory agRNAs.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Schwartz JC,Younger ST,Nguyen NB,Hardy DB,Monia BP,Corey DR,Janowski BAdoi
10.1038/nsmb.1444subject
Has Abstractpub_date
2008-08-01 00:00:00pages
842-8issue
8eissn
1545-9993issn
1545-9985pii
nsmb.1444journal_volume
15pub_type
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