Structure of a proton-dependent lipid transporter involved in lipoteichoic acids biosynthesis.

abstract：:Celiac disease is a T cell-mediated disease induced by dietary gluten, a component of which is gliadin. 95% of individuals with celiac disease carry the HLA (human leukocyte antigen)-DQ2 locus. Here we determined the T-cell receptor (TCR) usage and fine specificity of patient-derived T-cell clones specific for two epi...

journal_title：Nature structural & molecular biology

authors： Petersen J,Montserrat V,Mujico JR,Loh KL,Beringer DX,van Lummel M,Thompson A,Mearin ML,Schweizer J,Kooy-Winkelaar Y,van Bergen J,Drijfhout JW,Kan WT,La Gruta NL,Anderson RP,Reid HH,Koning F,Rossjohn J

更新日期：2014-05-01 00:00:00

abstract：:Allosteric interactions are typically considered to proceed through a series of discrete changes in bonding interactions that alter the protein conformation. Here we show that allostery can be mediated exclusively by transmitted changes in protein motions. We have characterized the negatively cooperative binding of cA...

journal_title：Nature structural & molecular biology

authors： Popovych N,Sun S,Ebright RH,Kalodimos CG

更新日期：2006-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Fichou Y,Han S

更新日期：2019-06-01 00:00:00

abstract：:By sequence-specific binding to 3' UUU-OH, the La protein shields precursor (pre)-RNAs from 3' end digestion and is required to protect defective pre-transfer RNAs from decay. Although La is comprised of a La motif and an RNA-recognition motif (RRM), a recent structure indicates that the RRM beta-sheet surface is not ...

journal_title：Nature structural & molecular biology

authors： Huang Y,Bayfield MA,Intine RV,Maraia RJ

更新日期：2006-07-01 00:00:00

abstract：:Catalysis by members of the RNase H superfamily of enzymes is generally believed to require only two Mg2+ ions that are coordinated by active-site carboxylates. By examining the catalytic process of Bacillus halodurans RNase H1 in crystallo, however, we found that the two canonical Mg2+ ions and an additional K+ faile...

journal_title：Nature structural & molecular biology

authors： Samara NL,Yang W

更新日期：2018-08-01 00:00:00

abstract：:The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates heterogeneous microtubules and affects their functions. Here we describe the crystal and solution structure of the tubulin carboxypeptidase complex between vasohibin (VASH1) and small vasohibin-binding protein (SVBP), which fol...

journal_title：Nature structural & molecular biology

authors： Adamopoulos A,Landskron L,Heidebrecht T,Tsakou F,Bleijerveld OB,Altelaar M,Nieuwenhuis J,Celie PHN,Brummelkamp TR,Perrakis A

更新日期：2019-07-01 00:00:00

abstract：:Histones have two structurally and functionally distinct domains: globular domains forming the nucleosomal core around which DNA is wrapped and unstructured tails protruding from the nucleosomal core. Whereas post-translational modifications (PTMs) in histone tails are well studied, much less is currently known about ...

journal_title：Nature structural & molecular biology

authors： Tropberger P,Schneider R

更新日期：2013-06-01 00:00:00

abstract：:Neuronal communication is mediated by Ca(2+)-triggered fusion of transmitter-filled synaptic vesicles with the presynaptic plasma membrane. Synaptotagmin I functions as a Ca(2+) sensor that regulates exocytosis, whereas soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins in th...

journal_title：Nature structural & molecular biology

authors： Chicka MC,Hui E,Liu H,Chapman ER

更新日期：2008-08-01 00:00:00

abstract：:Binding of the gp120 envelope (Env) glycoprotein to the CD4 receptor is the first step in the HIV-1 infectious cycle. Although the CD4-binding site has been extensively characterized, the initial receptor interaction has been difficult to study because of major CD4-induced structural rearrangements. Here we used cryog...

journal_title：Nature structural & molecular biology

authors： Liu Q,Acharya P,Dolan MA,Zhang P,Guzzo C,Lu J,Kwon A,Gururani D,Miao H,Bylund T,Chuang GY,Druz A,Zhou T,Rice WJ,Wigge C,Carragher B,Potter CS,Kwong PD,Lusso P

更新日期：2017-04-01 00:00:00

abstract：:We show that convergent transcription induces transcriptional gene silencing (TGS) in trans for both fission yeast and mammalian cells. This method has advantages over existing strategies to induce gene silencing. Previous studies in fission yeast have characterized TGS as a cis-specific process involving RNA interfer...

journal_title：Nature structural & molecular biology

authors： Gullerova M,Proudfoot NJ

更新日期：2012-11-01 00:00:00

abstract：:We investigated whether a continuous transcript is necessary for co-transcriptional pre-mRNA processing. Cutting an intron with the fast-cleaving hepatitis delta ribozyme, but not the slower hammerhead, inhibited splicing. Therefore, exon tethering to RNA polymerase II (Pol II) cannot rescue splicing of a transcript s...

journal_title：Nature structural & molecular biology

authors： Fong N,Ohman M,Bentley DL

更新日期：2009-09-01 00:00:00

abstract：:The CagA protein of Helicobacter pylori interacts with numerous cellular factors and is associated with increased virulence and risk of gastric carcinoma. We present here the cocrystal structure of a subdomain of CagA with the human kinase PAR1b/MARK2, revealing that a CagA peptide mimics substrates of this kinase fam...

journal_title：Nature structural & molecular biology

authors： Nesić D,Miller MC,Quinkert ZT,Stein M,Chait BT,Stebbins CE

更新日期：2010-01-01 00:00:00

abstract：:The general preprotein translocase of the outer mitochondrial membrane (TOM complex) transports virtually all mitochondrial precursor proteins, but cannot assemble outer-membrane precursors into functional complexes. A recently discovered sorting and assembly machinery (SAM complex) is essential for integration and as...

journal_title：Nature structural & molecular biology

authors： Pfanner N,Wiedemann N,Meisinger C,Lithgow T

更新日期：2004-11-01 00:00:00

abstract：:The asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a) acts as a repressive mark that antagonizes trimethylation of H3 lysine 4. Here we report that H3R2 is also symmetrically dimethylated (H3R2me2s) by PRMT5 and PRMT7 and present in euchromatic regions. Profiling of H3-tail interactors by SILAC MS revealed ...

journal_title：Nature structural & molecular biology

authors： Migliori V,Müller J,Phalke S,Low D,Bezzi M,Mok WC,Sahu SK,Gunaratne J,Capasso P,Bassi C,Cecatiello V,De Marco A,Blackstock W,Kuznetsov V,Amati B,Mapelli M,Guccione E

更新日期：2012-01-08 00:00:00

abstract：:The proteasome is the main ATP-dependent protease in eukaryotic cells and controls the concentration of many regulatory proteins in the cytosol and nucleus. Proteins are targeted to the proteasome by the covalent attachment of polyubiquitin chains. The ubiquitin modification serves as the proteasome recognition elemen...

journal_title：Nature structural & molecular biology

authors： Prakash S,Tian L,Ratliff KS,Lehotzky RE,Matouschek A

更新日期：2004-09-01 00:00:00

abstract：:The N-terminal acetylation of Sir3 is essential for heterochromatin establishment and maintenance in yeast, but its mechanism of action is unknown. The crystal structure of the N-terminally acetylated BAH domain of Saccharomyces cerevisiae Sir3 bound to the nucleosome core particle reveals that the N-terminal acetylat...

journal_title：Nature structural & molecular biology

authors： Arnaudo N,Fernández IS,McLaughlin SH,Peak-Chew SY,Rhodes D,Martino F

更新日期：2013-09-01 00:00:00

abstract：:The regulated exocytotic release of neurotransmitter and hormones is accomplished by a complex protein machinery whose core consists of SNARE proteins and the calcium sensor synaptotagmin-1. We propose a mechanism in which the lipid membrane is intimately involved in coupling calcium sensing to release. We found that ...

journal_title：Nature structural & molecular biology

authors： Kiessling V,Kreutzberger AJB,Liang B,Nyenhuis SB,Seelheim P,Castle JD,Cafiso DS,Tamm LK

更新日期：2018-10-01 00:00:00

abstract：:Natural chromosome ends resemble double-stranded DNA breaks, but they do not activate a damage response in healthy cells. Telomeres therefore have evolved to solve the 'end-protection problem' by inhibiting multiple DNA damage-response pathways. During the past decade, the view of telomeres has progressed from simple ...

journal_title：Nature structural & molecular biology

authors： Arnoult N,Karlseder J

更新日期：2015-11-01 00:00:00

abstract：:In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a ...

journal_title：Nature structural & molecular biology

authors： Weixlbaumer A,Petry S,Dunham CM,Selmer M,Kelley AC,Ramakrishnan V

更新日期：2007-08-01 00:00:00

abstract：:Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-ind...

journal_title：Nature structural & molecular biology

authors： Bühler M,Steiner S,Mohn F,Paillusson A,Mühlemann O

更新日期：2006-05-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Nicoll CR,Bailleul G,Fiorentini F,Mascotti ML,Fraaije MW,Mattevi A

更新日期：2020-02-01 00:00:00

abstract：:Glucokinase (GK) is a glucose-phosphorylating enzyme that regulates insulin release and hepatic metabolism, and its loss of function is implicated in diabetes pathogenesis. GK activators (GKAs) are attractive therapeutics in diabetes; however, clinical data indicate that their benefits can be offset by hypoglycemia, o...

journal_title：Nature structural & molecular biology

authors： Szlyk B,Braun CR,Ljubicic S,Patton E,Bird GH,Osundiji MA,Matschinsky FM,Walensky LD,Danial NN

更新日期：2014-01-01 00:00:00

abstract：:TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by...

journal_title：Nature structural & molecular biology

authors： Price AJ,Marzetta F,Lammers M,Ylinen LM,Schaller T,Wilson SJ,Towers GJ,James LC

更新日期：2009-10-01 00:00:00

abstract：:Suppressing cellular signal transducers of transcription 2 (STAT2) is a common strategy that viruses use to establish infections, yet the detailed mechanism remains elusive, owing to a lack of structural information about the viral-cellular complex involved. Here, we report the cryo-EM and crystal structures of human ...

journal_title：Nature structural & molecular biology

authors： Wang B,Thurmond S,Zhou K,Sánchez-Aparicio MT,Fang J,Lu J,Gao L,Ren W,Cui Y,Veit EC,Hong H,Evans MJ,O'Leary SE,García-Sastre A,Zhou ZH,Hai R,Song J

更新日期：2020-10-01 00:00:00

abstract：:The polypyrimidine tract binding protein (PTB) binds pre-mRNAs to alter splice-site choice. We characterized a series of spliceosomal complexes that assemble on a pre-mRNA under conditions of either PTB-mediated splicing repression or its absence. In the absence of repression, exon definition complexes that were assem...

journal_title：Nature structural & molecular biology

authors： Sharma S,Kohlstaedt LA,Damianov A,Rio DC,Black DL

更新日期：2008-02-01 00:00:00

abstract：:Myosin V is a double-headed processive molecular motor that moves along an actin filament by taking 36-nm steps. Using optical trapping nanometry with high spatiotemporal resolution, we discovered that there are two possible pathways for the 36-nm steps, one with 12- and 24-nm substeps, in this order, and the other wi...

journal_title：Nature structural & molecular biology

authors： Uemura S,Higuchi H,Olivares AO,De La Cruz EM,Ishiwata S

更新日期：2004-09-01 00:00:00

abstract：:RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the bind...

journal_title：Nature structural & molecular biology

authors： Kassube SA,Jinek M,Fang J,Tsutakawa S,Nogales E

更新日期：2013-07-01 00:00:00

abstract：:The GTPase dynamin is essential for numerous vesiculation events including clathrin-mediated endocytosis. Upon GTP hydrolysis, dynamin constricts a lipid bilayer. Previously, a three-dimensional structure of mutant dynamin in the constricted state was determined by helical reconstruction methods. We solved the noncons...

journal_title：Nature structural & molecular biology

authors： Chen YJ,Zhang P,Egelman EH,Hinshaw JE

更新日期：2004-06-01 00:00:00

abstract：:The histone variant macroH2A1 regulates gene expression important for differentiation, stem-cell reprogramming and tumor suppression. Here, we demonstrate that in primary human cells, macroH2A1 participates in two physically and functionally distinct types of chromatin marked by either H3K27me3 or nine histone acetyla...

journal_title：Nature structural & molecular biology

authors： Chen H,Ruiz PD,Novikov L,Casill AD,Park JW,Gamble MJ

更新日期：2014-11-01 00:00:00

abstract：:Many viruses bypass canonical cap-dependent translation in host cells by using internal ribosomal entry sites (IRESs) in their transcripts; IRESs hijack initiation factors for the assembly of initiation complexes. However, it is currently unknown how IRES RNAs recognize initiation factors that have no endogenous RNA b...

journal_title：Nature structural & molecular biology

authors： Imai S,Kumar P,Hellen CU,D'Souza VM,Wagner G

更新日期：2016-09-01 00:00:00