Abstract:
:Thymic stromal lymphopoietin (TSLP), a cytokine produced by epithelial cells at barrier surfaces, is pivotal for the development of widespread chronic inflammatory disorders such as asthma and atopic dermatitis. The structure of the mouse TSLP-mediated signaling complex reveals how TSLP establishes extensive interfaces with its cognate receptor (TSLPR) and the shared interleukin 7 receptor α-chain (IL-7Rα) to evoke membrane-proximal receptor-receptor contacts poised for intracellular signaling. Binding of TSLP to TSLPR is a mechanistic prerequisite for recruitment of IL-7Rα to the high-affinity ternary complex, which we propose is coupled to a structural switch in TSLP at the crossroads of the cytokine-receptor interfaces. Functional interrogation of TSLP-receptor interfaces points to putative interaction hotspots that could be exploited for antagonist design. Finally, we derive the structural rationale for the functional duality of IL-7Rα and establish a consensus for the geometry of ternary complexes mediated by interleukin 2 (IL-2)-family cytokines.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Verstraete K,van Schie L,Vyncke L,Bloch Y,Tavernier J,Pauwels E,Peelman F,Savvides SNdoi
10.1038/nsmb.2794subject
Has Abstractpub_date
2014-04-01 00:00:00pages
375-82issue
4eissn
1545-9993issn
1545-9985pii
nsmb.2794journal_volume
21pub_type
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