Abstract:
:Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using alpha-amanitin-resistant pol II CTD mutants and fibronectin reporter minigenes. We found that the CTD is required for the inhibitory action of the serine/arginine-rich (SR) protein SRp20 on the inclusion of a fibronectin cassette exon in the mature mRNA. CTD phosphorylation controls transcription elongation, which is a major contributor to alternative splicing regulation. However, the effect of SRp20 is still observed when transcription elongation is reduced. These results suggest that the CTD promotes exon skipping by recruiting SRp20 and that this contributes independently of elongation to the transcriptional control of alternative splicing.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
de la Mata M,Kornblihtt ARdoi
10.1038/nsmb1155subject
Has Abstractpub_date
2006-11-01 00:00:00pages
973-80issue
11eissn
1545-9993issn
1545-9985pii
nsmb1155journal_volume
13pub_type
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