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An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome.

Abstract：

:Simultaneous profiling of transcriptome and chromatin accessibility within single cells is a powerful approach to dissect gene regulatory programs in complex tissues. However, current tools are limited by modest throughput. We now describe an ultra high-throughput method, Paired-seq, for parallel analysis of transcriptome and accessible chromatin in millions of single cells. We demonstrate the utility of Paired-seq for analyzing the dynamic and cell-type-specific gene regulatory programs in complex tissues by applying it to mouse adult cerebral cortex and fetal forebrain. The joint profiles of a large number of single cells allowed us to deconvolute the transcriptome and open chromatin landscapes in the major cell types within these brain tissues, infer putative target genes of candidate enhancers, and reconstruct the trajectory of cellular lineages within the developing forebrain.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Zhu C,Yu M,Huang H,Juric I,Abnousi A,Hu R,Lucero J,Behrens MM,Hu M,Ren B

doi

10.1038/s41594-019-0323-x

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

1063-1070

issue

11

eissn

1545-9993

issn

1545-9985

pii

10.1038/s41594-019-0323-x

journal_volume

26

pub_type

杂志文章

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