Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region.

abstract：:Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How these events are coordinated with the cell cycle is poorly understood, particularly in mammalian cells. We report here, based on newly developed techniques for visualizing NPC formation,...

journal_title：Nature structural & molecular biology

authors： Maeshima K,Iino H,Hihara S,Funakoshi T,Watanabe A,Nishimura M,Nakatomi R,Yahata K,Imamoto F,Hashikawa T,Yokota H,Imamoto N

更新日期：2010-09-01 00:00:00

abstract：:During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1...

journal_title：Nature structural & molecular biology

authors： Ito K,Murayama Y,Takahashi M,Iwasaki H

更新日期：2018-01-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:The normally soluble TAR DNA-binding protein 43 (TDP-43) is found aggregated both in reversible stress granules and in irreversible pathogenic amyloid. In TDP-43, the low-complexity domain (LCD) is believed to be involved in both types of aggregation. To uncover the structural origins of these two modes of β-sheet-ric...

journal_title：Nature structural & molecular biology

authors： Guenther EL,Cao Q,Trinh H,Lu J,Sawaya MR,Cascio D,Boyer DR,Rodriguez JA,Hughes MP,Eisenberg DS

更新日期：2018-06-01 00:00:00

abstract：:Spliceosomes catalyze the maturation of precursor mRNAs in organisms ranging from yeast to humans. Their catalytic core comprises three small nuclear RNAs (U2, U5 and U6) involved in substrate positioning and catalysis. It has been postulated, but never shown experimentally, that the U2-U6 complex adopts at least two ...

journal_title：Nature structural & molecular biology

authors： Guo Z,Karunatilaka KS,Rueda D

更新日期：2009-11-01 00:00:00

abstract：:Glucokinase (GK) is a glucose-phosphorylating enzyme that regulates insulin release and hepatic metabolism, and its loss of function is implicated in diabetes pathogenesis. GK activators (GKAs) are attractive therapeutics in diabetes; however, clinical data indicate that their benefits can be offset by hypoglycemia, o...

journal_title：Nature structural & molecular biology

authors： Szlyk B,Braun CR,Ljubicic S,Patton E,Bird GH,Osundiji MA,Matschinsky FM,Walensky LD,Danial NN

更新日期：2014-01-01 00:00:00

abstract：:Self-templating assemblies of the human prion protein are clinically associated with transmissible spongiform encephalopathies. Here we present the cryo-EM structure of a denaturant- and protease-resistant fibril formed in vitro spontaneously by a 9.7-kDa unglycosylated fragment of the human prion protein. This human ...

journal_title：Nature structural & molecular biology

authors： Glynn C,Sawaya MR,Ge P,Gallagher-Jones M,Short CW,Bowman R,Apostol M,Zhou ZH,Eisenberg DS,Rodriguez JA

更新日期：2020-05-01 00:00:00

abstract：:Green tea has held a long-standing place in traditional Asian medicine. Scientific research is now beginning to explain why. ...

journal_title：Nature structural & molecular biology

authors：

更新日期：2008-06-01 00:00:00

abstract：:We describe a novel approach to separate two ribosome populations from the same cells and use this method in combination with RNA-seq to identify mRNAs bound to Saccharomyces cerevisiae ribosomes with and without Rps26, a protein linked to the pathogenesis of Diamond-Blackfan anemia (DBA). These analyses reveal that R...

journal_title：Nature structural & molecular biology

authors： Ferretti MB,Ghalei H,Ward EA,Potts EL,Karbstein K

更新日期：2017-09-01 00:00:00

abstract：:RNA functions at enhancers remain mysterious. Here we show that the 7SK small nuclear RNA (snRNA) inhibits enhancer transcription by modulating nucleosome position. 7SK occupies enhancers and super enhancers genome wide in mouse and human cells, and it is required to limit enhancer-RNA initiation and synthesis in a ma...

journal_title：Nature structural & molecular biology

authors： Flynn RA,Do BT,Rubin AJ,Calo E,Lee B,Kuchelmeister H,Rale M,Chu C,Kool ET,Wysocka J,Khavari PA,Chang HY

更新日期：2016-03-01 00:00:00

abstract：:Many classes of small RNA (sRNA) involved in RNA silencing are generated by double-stranded RNA (dsRNA) processing. Although principles of sRNA biogenesis have emerged, newly identified classes of sRNAs have features that suggest additional biogenesis mechanisms. Tetrahymena thermophila expresses one such class, compr...

journal_title：Nature structural & molecular biology

authors： Lee SR,Collins K

更新日期：2007-07-01 00:00:00

abstract：:Aggregation of proteins containing polyglutamine (polyQ) expansions characterizes many neurodegenerative disorders, including Huntington's disease. Molecular chaperones modulate the aggregation and toxicity of the huntingtin (Htt) protein by an ill-defined mechanism. Here we determine how the chaperonin TRiC suppresse...

journal_title：Nature structural & molecular biology

authors： Tam S,Spiess C,Auyeung W,Joachimiak L,Chen B,Poirier MA,Frydman J

更新日期：2009-12-01 00:00:00

abstract：:The asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a) acts as a repressive mark that antagonizes trimethylation of H3 lysine 4. Here we report that H3R2 is also symmetrically dimethylated (H3R2me2s) by PRMT5 and PRMT7 and present in euchromatic regions. Profiling of H3-tail interactors by SILAC MS revealed ...

journal_title：Nature structural & molecular biology

authors： Migliori V,Müller J,Phalke S,Low D,Bezzi M,Mok WC,Sahu SK,Gunaratne J,Capasso P,Bassi C,Cecatiello V,De Marco A,Blackstock W,Kuznetsov V,Amati B,Mapelli M,Guccione E

更新日期：2012-01-08 00:00:00

abstract：:In response to stress, cells attenuate global protein synthesis but permit efficient translation of mRNAs encoding heat-shock proteins (HSPs). Although decades have passed since the first description of the heat-shock response, how cells achieve translational control of HSP synthesis remains enigmatic. Here we report ...

journal_title：Nature structural & molecular biology

authors： Zhang X,Gao X,Coots RA,Conn CS,Liu B,Qian SB

更新日期：2015-05-01 00:00:00

abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

journal_title：Nature structural & molecular biology

authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

更新日期：2012-06-24 00:00:00

abstract：:Neuronal communication is mediated by Ca(2+)-triggered fusion of transmitter-filled synaptic vesicles with the presynaptic plasma membrane. Synaptotagmin I functions as a Ca(2+) sensor that regulates exocytosis, whereas soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins in th...

journal_title：Nature structural & molecular biology

authors： Chicka MC,Hui E,Liu H,Chapman ER

更新日期：2008-08-01 00:00:00

abstract：:Proteins regulate gene expression by controlling mRNA biogenesis, localization, translation and decay. Identifying the composition, diversity and function of mRNA-protein complexes (mRNPs) is essential to understanding these processes. In a global survey of Saccharomyces cerevisiae mRNA-binding proteins, we identified...

journal_title：Nature structural & molecular biology

authors： Mitchell SF,Jain S,She M,Parker R

更新日期：2013-01-01 00:00:00

abstract：:The N-end rule pathway is a regulated proteolytic system that targets proteins containing destabilizing N-terminal residues (N-degrons) for ubiquitination and proteasomal degradation in eukaryotes. The N-degrons of type 1 substrates contain an N-terminal basic residue that is recognized by the UBR box domain of the E3...

journal_title：Nature structural & molecular biology

authors： Choi WS,Jeong BC,Joo YJ,Lee MR,Kim J,Eck MJ,Song HK

更新日期：2010-10-01 00:00:00

abstract：: ...

journal_title：Nature structural & molecular biology

authors： Horowitz S,Salmon L,Koldewey P,Ahlstrom LS,Martin R,Quan S,Afonine PV,van den Bedem H,Wang L,Xu Q,Trievel RC,Brooks CL 3rd,Bardwell JCA

更新日期：2018-11-01 00:00:00

abstract：:The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation. To distinguish between local displacement versus global unfolding mechanisms for complex disassem...

journal_title：Nature structural & molecular biology

authors： Yang B,Stjepanovic G,Shen Q,Martin A,Hurley JH

更新日期：2015-06-01 00:00:00

abstract：:Mycobacterium tuberculosis uses a proteasome system that is analogous to the eukaryotic ubiquitin-proteasome pathway and is required for pathogenesis. However, the bacterial analog of ubiquitin, prokaryotic ubiquitin-like protein (Pup), is an intrinsically disordered protein that bears little sequence or structural re...

journal_title：Nature structural & molecular biology

authors： Wang T,Darwin KH,Li H

更新日期：2010-11-01 00:00:00

abstract：:Thousands of long noncoding RNAs (lncRNAs) have been discovered, but their functional characterization has been slowed by a limited set of research tools. Here we review emerging RNA-centric methods to interrogate the intrinsic structure of lncRNAs as well as their genomic localization and biochemical partners. Unders...

journal_title：Nature structural & molecular biology

authors： Chu C,Spitale RC,Chang HY

更新日期：2015-01-01 00:00:00

abstract：:Mg-ADP release is considered to be a crucial process for the regulation and motility of kinesin. To gain insight into the structural basis of this process, we solved the atomic structures of kinesin superfamily protein-1A (KIF1A) during and after Mg(2+) release. On the basis of new structural and mutagenesis data, we ...

journal_title：Nature structural & molecular biology

authors： Nitta R,Okada Y,Hirokawa N

更新日期：2008-10-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Huo J,Le Bas A,Ruza RR,Duyvesteyn HME,Mikolajek H,Malinauskas T,Tan TK,Rijal P,Dumoux M,Ward PN,Ren J,Zhou D,Harrison PJ,Weckener M,Clare DK,Vogirala VK,Radecke J,Moynié L,Zhao Y,Gilbert-Jaramillo J,Knight ML,Tr

更新日期：2020-11-01 00:00:00

abstract：:Translation has a fundamental function in defining the fate of the transcribed genome. RNA-sequencing (RNA-seq) data enable the quantification of complex transcript mixtures, often detecting several transcript isoforms of unknown functions for one gene. Here, we describe ORFquant, a method to annotate and quantify tra...

journal_title：Nature structural & molecular biology

authors： Calviello L,Hirsekorn A,Ohler U

更新日期：2020-08-01 00:00:00

abstract：:TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by...

journal_title：Nature structural & molecular biology

authors： Price AJ,Marzetta F,Lammers M,Ylinen LM,Schaller T,Wilson SJ,Towers GJ,James LC

更新日期：2009-10-01 00:00:00

abstract：:The Saccharomyces cerevisiae proteasome comprises a 19-subunit regulatory particle and a 28-subunit core particle. To be degraded, substrates must cross the core particle-regulatory particle interface, a site for complex conformational changes and regulatory events. This interface includes two aligned heteromeric ring...

journal_title：Nature structural & molecular biology

authors： Tian G,Park S,Lee MJ,Huck B,McAllister F,Hill CP,Gygi SP,Finley D

更新日期：2011-10-30 00:00:00

abstract：:The GTPase dynamin is essential for numerous vesiculation events including clathrin-mediated endocytosis. Upon GTP hydrolysis, dynamin constricts a lipid bilayer. Previously, a three-dimensional structure of mutant dynamin in the constricted state was determined by helical reconstruction methods. We solved the noncons...

journal_title：Nature structural & molecular biology

authors： Chen YJ,Zhang P,Egelman EH,Hinshaw JE

更新日期：2004-06-01 00:00:00

abstract：:Newly synthesized polypeptides undergo various cotranslational maturation steps, including N-terminal enzymatic processing, chaperone-assisted folding and membrane targeting, but the spatial and temporal coordination of these steps is unclear. We show that Escherichia coli methionine aminopeptidase (MAP) associates wi...

journal_title：Nature structural & molecular biology

authors： Sandikci A,Gloge F,Martinez M,Mayer MP,Wade R,Bukau B,Kramer G

更新日期：2013-07-01 00:00:00

abstract：:The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its ...

journal_title：Nature structural & molecular biology

authors： Yamada T,Yoshida T,Kawamoto A,Mitsuoka K,Iwasaki K,Tsuge H

更新日期：2020-03-01 00:00:00