Proton-powered subunit rotation in single membrane-bound F0F1-ATP synthase.

abstract：:The DNA-damage response (DDR) ensures genome stability and proper inheritance of genetic information, both of which are essential to survival. It is presently unclear to what extent other signaling pathways modulate DDR function. Here we show that Notch receptor binds and inactivates ATM kinase and that this mechanism...

journal_title：Nature structural & molecular biology

authors： Vermezovic J,Adamowicz M,Santarpia L,Rustighi A,Forcato M,Lucano C,Massimiliano L,Costanzo V,Bicciato S,Del Sal G,d'Adda di Fagagna F

更新日期：2015-05-01 00:00:00

abstract：:We have determined the solution structure of the complex between an arginine-glycine-rich RGG peptide from the human fragile X mental retardation protein (FMRP) and an in vitro-selected guanine-rich (G-rich) sc1 RNA. The bound RNA forms a newly discovered G-quadruplex separated from the flanking duplex stem by a mixed...

journal_title：Nature structural & molecular biology

authors： Phan AT,Kuryavyi V,Darnell JC,Serganov A,Majumdar A,Ilin S,Raslin T,Polonskaia A,Chen C,Clain D,Darnell RB,Patel DJ

更新日期：2011-06-05 00:00:00

abstract：:Platelet-activating-factor receptor (PAFR) responds to platelet-activating factor (PAF), a phospholipid mediator of cell-to-cell communication that exhibits diverse physiological effects. PAFR is considered an important drug target for treating asthma, inflammation and cardiovascular diseases. Here we report crystal s...

journal_title：Nature structural & molecular biology

authors： Cao C,Tan Q,Xu C,He L,Yang L,Zhou Y,Zhou Y,Qiao A,Lu M,Yi C,Han GW,Wang X,Li X,Yang H,Rao Z,Jiang H,Zhao Y,Liu J,Stevens RC,Zhao Q,Zhang XC,Wu B

更新日期：2018-06-01 00:00:00

abstract：:Thousands of long noncoding RNAs (lncRNAs) have been discovered, but their functional characterization has been slowed by a limited set of research tools. Here we review emerging RNA-centric methods to interrogate the intrinsic structure of lncRNAs as well as their genomic localization and biochemical partners. Unders...

journal_title：Nature structural & molecular biology

authors： Chu C,Spitale RC,Chang HY

更新日期：2015-01-01 00:00:00

abstract：:Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviat...

journal_title：Nature structural & molecular biology

authors： Bange G,Kümmerer N,Grudnik P,Lindner R,Petzold G,Kressler D,Hurt E,Wild K,Sinning I

更新日期：2011-11-06 00:00:00

abstract：:Many aspects of nucleic acid metabolism, such as DNA replication, repair and transcription, are regulated by the post-translational modifiers ubiquitin and SUMO. Not surprisingly, DNA itself plays an integral part in determining the modification of most chromatin-associated targets. Conversely, ubiquitination or SUMOy...

journal_title：Nature structural & molecular biology

authors： Ulrich HD

更新日期：2014-04-01 00:00:00

abstract：:Chromatin is a dynamic structure that must respond to myriad stimuli to regulate access to DNA, and chemical modification of histones is a major means by which the cell modulates nucleosome mobility and turnover. Histone modifications are linked to essentially every cellular process requiring DNA access, including tra...

journal_title：Nature structural & molecular biology

authors： Zentner GE,Henikoff S

更新日期：2013-03-01 00:00:00

abstract：:The Rbfox family of splicing factors regulate alternative splicing during animal development and in disease, impacting thousands of exons in the maturing brain, heart and muscle. Rbfox proteins have long been known to bind to the RNA sequence GCAUG with high affinity and specificity, but just half of Rbfox binding sit...

journal_title：Nature structural & molecular biology

authors： Begg BE,Jens M,Wang PY,Minor CM,Burge CB

更新日期：2020-10-01 00:00:00

abstract：:The core of the neurotransmitter release machinery is formed by SNARE complexes, which bring the vesicle and plasma membranes together and are key for fusion, and by Munc18-1, which controls SNARE-complex formation and may also have a direct role in fusion. In addition, SNARE complex assembly is likely orchestrated by...

journal_title：Nature structural & molecular biology

authors： Rizo J,Rosenmund C

更新日期：2008-07-01 00:00:00

abstract：:Otopetrins (Otop1-Otop3) comprise one of two known eukaryotic proton-selective channel families. Otop1 is required for otoconia formation and a candidate mammalian sour taste receptor. Here we report cryo-EM structures of zebrafish Otop1 and chicken Otop3 in lipid nanodiscs. The structures reveal a dimeric architectur...

journal_title：Nature structural & molecular biology

authors： Saotome K,Teng B,Tsui CCA,Lee WH,Tu YH,Kaplan JP,Sansom MSP,Liman ER,Ward AB

更新日期：2019-06-01 00:00:00

abstract：:Processing of microRNAs (miRNAs) from their precursors to their biologically active mature forms is regulated during development and cancer. We show that mouse pri- or pre-miR-151 can bind to and compete with mature miR-151-5p and miR-151-3p for binding sites contained within the complementary regions of the E2f6 mRNA...

journal_title：Nature structural & molecular biology

authors： Roy-Chaudhuri B,Valdmanis PN,Zhang Y,Wang Q,Luo QJ,Kay MA

更新日期：2014-09-01 00:00:00

abstract：:We present a structural analysis of the folding transition states of three SH3 domains. Our results reveal that the secondary structure is not yet fully formed at this stage of folding and that the solvent is only partially excluded from the interior of the protein. Comparison of the members of the transition state en...

journal_title：Nature structural & molecular biology

authors： Lindorff-Larsen K,Vendruscolo M,Paci E,Dobson CM

更新日期：2004-05-01 00:00:00

abstract：:CpG island-like sequences are commonly thought to provide the sole signals for designating constitutively unmethylated regions in the genome, thus generating open chromatin domains within a sea of global repression. Using a new database obtained from comprehensive microarray analysis, we show that unmethylated regions...

journal_title：Nature structural & molecular biology

authors： Straussman R,Nejman D,Roberts D,Steinfeld I,Blum B,Benvenisty N,Simon I,Yakhini Z,Cedar H

更新日期：2009-05-01 00:00:00

abstract：:Post-translational modification of proteins by ubiquitin (Ub) and Ub-like modifiers regulates DNA replication. We have previously shown that chromatin around replisomes is rich in SUMO and poor in Ub, whereas mature chromatin exhibits an opposite pattern. How this SUMO-rich, Ub-poor environment is maintained at sites ...

journal_title：Nature structural & molecular biology

authors： Lecona E,Rodriguez-Acebes S,Specks J,Lopez-Contreras AJ,Ruppen I,Murga M,Muñoz J,Mendez J,Fernandez-Capetillo O

更新日期：2016-04-01 00:00:00

abstract：:Trimethylation of Lys36 in histone H3 (H3K36me3) coordinates events associated with the elongation phase of transcription and is also emerging as an important epigenetic regulator of cell growth and differentiation. We have identified the PWWP domain of bromo and plant homeodomain (PHD) finger-containing protein 1 (BR...

journal_title：Nature structural & molecular biology

authors： Vezzoli A,Bonadies N,Allen MD,Freund SM,Santiveri CM,Kvinlaug BT,Huntly BJ,Göttgens B,Bycroft M

更新日期：2010-05-01 00:00:00

abstract：:Primary microRNA cleavage by the Drosha-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the au...

journal_title：Nature structural & molecular biology

authors： Barad O,Mann M,Chapnik E,Shenoy A,Blelloch R,Barkai N,Hornstein E

更新日期：2012-05-13 00:00:00

abstract：:Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using alpha-amanitin-resistant pol II CTD mutants and fibrone...

journal_title：Nature structural & molecular biology

authors： de la Mata M,Kornblihtt AR

更新日期：2006-11-01 00:00:00

abstract：:Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can ...

journal_title：Nature structural & molecular biology

authors： Sarlós K,Biebricher AS,Bizard AH,Bakx JAM,Ferreté-Bonastre AG,Modesti M,Paramasivam M,Yao Q,Peterman EJG,Wuite GJL,Hickson ID

更新日期：2018-09-01 00:00:00

abstract：:Mutually exclusive splicing is a regulated means to generate protein diversity, but the underlying mechanisms are poorly understood. Here comparative genome analysis revealed the built-in intronic elements for controlling mutually exclusive splicing of the 14-3-3ξ pre-mRNA. These elements are clade specific but are ev...

journal_title：Nature structural & molecular biology

authors： Yang Y,Zhan L,Zhang W,Sun F,Wang W,Tian N,Bi J,Wang H,Shi D,Jiang Y,Zhang Y,Jin Y

更新日期：2011-02-01 00:00:00

abstract：:Protein quality control depends on the tight regulation of interactions between molecular chaperones and polypeptide substrates. Substrate release from the chaperone Hsp70 is triggered by nucleotide-exchange factors (NEFs) that control folding and degradation fates via poorly understood mechanisms. We found that the a...

journal_title：Nature structural & molecular biology

authors： Gowda NKC,Kaimal JM,Kityk R,Daniel C,Liebau J,Öhman M,Mayer MP,Andréasson C

更新日期：2018-01-01 00:00:00

abstract：:Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJC and the translation machinery has not been identified. The EJC prot...

journal_title：Nature structural & molecular biology

authors： Diem MD,Chan CC,Younis I,Dreyfuss G

更新日期：2007-12-01 00:00:00

abstract：:How do the key features of protein folding, elucidated from studies on native, isolated proteins, manifest in cotranslational folding on the ribosome? Using a well-characterized family of homologous α-helical proteins with a range of biophysical properties, we show that spectrin domains can fold vectorially on the rib...

journal_title：Nature structural & molecular biology

authors： Nilsson OB,Nickson AA,Hollins JJ,Wickles S,Steward A,Beckmann R,von Heijne G,Clarke J

更新日期：2017-03-01 00:00:00

abstract：:The CagA protein of Helicobacter pylori interacts with numerous cellular factors and is associated with increased virulence and risk of gastric carcinoma. We present here the cocrystal structure of a subdomain of CagA with the human kinase PAR1b/MARK2, revealing that a CagA peptide mimics substrates of this kinase fam...

journal_title：Nature structural & molecular biology

authors： Nesić D,Miller MC,Quinkert ZT,Stein M,Chait BT,Stebbins CE

更新日期：2010-01-01 00:00:00

abstract：:The monoclonal antibody 13H11 shares part of its epitope in the HIV-1 gp41 membrane-proximal external region (MPER) with the rare, broadly neutralizing human antibody 2F5. Although 13H11 partially cross-blocked 2F5 binding, 13H11 is non-neutralizing and does not block 2F5 neutralization. We show that unlike 2F5, 13H11...

journal_title：Nature structural & molecular biology

authors： Nicely NI,Dennison SM,Spicer L,Scearce RM,Kelsoe G,Ueda Y,Chen H,Liao HX,Alam SM,Haynes BF

更新日期：2010-12-01 00:00:00

abstract：:The ubiquitination cascade has a key role in the assembly of repair and signaling proteins at sites of double-strand DNA breaks. The E3 ubiquitin ligase RING finger protein 8 (RNF8) triggers the initial ubiquitination at double-strand DNA breaks, whereas sustained ubiquitination requires the downstream E3 ligase RING ...

journal_title：Nature structural & molecular biology

authors： Feng L,Chen J

更新日期：2012-01-22 00:00:00

abstract：:Aggregation of proteins containing polyglutamine (polyQ) expansions characterizes many neurodegenerative disorders, including Huntington's disease. Molecular chaperones modulate the aggregation and toxicity of the huntingtin (Htt) protein by an ill-defined mechanism. Here we determine how the chaperonin TRiC suppresse...

journal_title：Nature structural & molecular biology

authors： Tam S,Spiess C,Auyeung W,Joachimiak L,Chen B,Poirier MA,Frydman J

更新日期：2009-12-01 00:00:00

abstract：:Histones have two structurally and functionally distinct domains: globular domains forming the nucleosomal core around which DNA is wrapped and unstructured tails protruding from the nucleosomal core. Whereas post-translational modifications (PTMs) in histone tails are well studied, much less is currently known about ...

journal_title：Nature structural & molecular biology

authors： Tropberger P,Schneider R

更新日期：2013-06-01 00:00:00

abstract：:The tumor suppressor p53 has been proposed to bind target promoters upon genotoxic stress. However, recent evidence shows that p53 occupies some target promoters without such stress, suggesting that a negative regulator might render p53 transcriptionally inactive on these promoters. Here we show that calcineurin bindi...

journal_title：Nature structural & molecular biology

authors： Jang H,Choi SY,Cho EJ,Youn HD

更新日期：2009-09-01 00:00:00

abstract：:Coupling between transcription and RNA processing is a key gene regulatory mechanism. Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation of the precursor mRNA (pre-mRNA) splicing factors hnRNP A1, U2AF65 and U1 and U5 snRNPs on the intron-containing human FOS gene. These factors w...

journal_title：Nature structural & molecular biology

authors： Listerman I,Sapra AK,Neugebauer KM

更新日期：2006-09-01 00:00:00

abstract：:Celiac disease is a T cell-mediated disease induced by dietary gluten, a component of which is gliadin. 95% of individuals with celiac disease carry the HLA (human leukocyte antigen)-DQ2 locus. Here we determined the T-cell receptor (TCR) usage and fine specificity of patient-derived T-cell clones specific for two epi...

journal_title：Nature structural & molecular biology

authors： Petersen J,Montserrat V,Mujico JR,Loh KL,Beringer DX,van Lummel M,Thompson A,Mearin ML,Schweizer J,Kooy-Winkelaar Y,van Bergen J,Drijfhout JW,Kan WT,La Gruta NL,Anderson RP,Reid HH,Koning F,Rossjohn J

更新日期：2014-05-01 00:00:00