Abstract:
:Integral membrane proteins are generally believed to have unique membrane topologies. However, it has been suggested that dual-topology proteins that adopt a mixture of two opposite orientations in the membrane may exist. Here we show that the membrane orientations of five dual-topology candidates identified in Escherichia coli are highly sensitive to changes in the distribution of positively charged residues, that genes in families containing dual-topology candidates occur in genomes either as pairs or as singletons and that gene pairs encode two oppositely oriented proteins whereas singletons encode dual-topology candidates. Our results provide strong support for the existence of dual-topology proteins and shed new light on the evolution of membrane-protein topology and structure.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Rapp M,Granseth E,Seppälä S,von Heijne Gdoi
10.1038/nsmb1057keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
112-6issue
2eissn
1545-9993issn
1545-9985pii
nsmb1057journal_volume
13pub_type
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