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RNA polymerase II pauses and associates with pre-mRNA processing factors at both ends of genes.

Abstract：

:We investigated co-transcriptional recruitment of pre-mRNA processing factors to human genes. Capping factors associate with paused RNA polymerase II (pol II) at the 5' ends of quiescent genes. They also track throughout actively transcribed genes and accumulate with paused polymerase in the 3' flanking region. The 3' processing factors cleavage stimulation factor and cleavage polyadenylation specificity factor are maximally recruited 0.5-1.5 kilobases downstream of poly(A) sites where they coincide with capping factors, Spt5, and Ser2-hyperphosphorylated, paused pol II. 3' end processing factors also localize at transcription start sites, and this early recruitment is enhanced after polymerase arrest with the elongation factor DRB. These results suggest that promoters may help specify recruitment of 3' end processing factors. We propose a dual-pausing model wherein elongation arrests near the transcription start site and in the 3' flank to allow co-transcriptional processing by factors recruited to the pol II ternary complex.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Glover-Cutter K,Kim S,Espinosa J,Bentley DL

doi

10.1038/nsmb1352

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

71-8

issue

1

eissn

1545-9993

issn

1545-9985

pii

nsmb1352

journal_volume

15

pub_type

杂志文章

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