Fast ribozyme cleavage releases transcripts from RNA polymerase II and aborts co-transcriptional pre-mRNA processing.

abstract：:Complex forms of cellular motility, including cell division, organelle trafficking or signal amplification in the auditory system, require strong coordination of the myosin motors involved. The most basic mechanism of coordination is via direct mechanical interactions of individual motor heads leading to modification ...

journal_title：Nature structural & molecular biology

authors： Sellers JR,Veigel C

更新日期：2010-05-01 00:00:00

abstract：:The spliceosome is thought to undergo a conformational change between the two catalytic steps of precursor messenger RNA splicing, although the specific events in this transition are poorly understood. We previously proposed a two-state model of splicing in which the conformations required for the first and second ste...

journal_title：Nature structural & molecular biology

authors： Liu L,Query CC,Konarska MM

更新日期：2007-06-01 00:00:00

abstract：:Brr2 is a DExD/H-box helicase responsible for U4/U6 unwinding during spliceosomal activation. Brr2 contains two helicase-like domains, each of which is followed by a Sec63 domain with unknown function. We determined the crystal structure of the second Sec63 domain, which unexpectedly resembles domains 4 and 5 of DNA h...

journal_title：Nature structural & molecular biology

authors： Zhang L,Xu T,Maeder C,Bud LO,Shanks J,Nix J,Guthrie C,Pleiss JA,Zhao R

更新日期：2009-07-01 00:00:00

abstract：:Obesity and type II diabetes are closely linked metabolic syndromes that afflict >100 million people worldwide. Although protein tyrosine phosphatase 1B (PTP1B) has emerged as a promising target for the treatment of both syndromes, the discovery of pharmaceutically acceptable inhibitors that bind at the active site re...

journal_title：Nature structural & molecular biology

authors： Wiesmann C,Barr KJ,Kung J,Zhu J,Erlanson DA,Shen W,Fahr BJ,Zhong M,Taylor L,Randal M,McDowell RS,Hansen SK

更新日期：2004-08-01 00:00:00

abstract：:The antiviral and antiproliferative effects of interferons are mediated in part by the 2'-5' oligoadenylate-RNase L RNA decay pathway. RNase L is an endoribonuclease that requires 2'-5' oligoadenylates to cleave single-stranded RNA. In this report we present evidence demonstrating a role for RNase L in translation. We...

journal_title：Nature structural & molecular biology

authors： Le Roy F,Salehzada T,Bisbal C,Dougherty JP,Peltz SW

更新日期：2005-06-01 00:00:00

abstract：:Trimethylation of Lys36 in histone H3 (H3K36me3) coordinates events associated with the elongation phase of transcription and is also emerging as an important epigenetic regulator of cell growth and differentiation. We have identified the PWWP domain of bromo and plant homeodomain (PHD) finger-containing protein 1 (BR...

journal_title：Nature structural & molecular biology

authors： Vezzoli A,Bonadies N,Allen MD,Freund SM,Santiveri CM,Kvinlaug BT,Huntly BJ,Göttgens B,Bycroft M

更新日期：2010-05-01 00:00:00

abstract：:How do the key features of protein folding, elucidated from studies on native, isolated proteins, manifest in cotranslational folding on the ribosome? Using a well-characterized family of homologous α-helical proteins with a range of biophysical properties, we show that spectrin domains can fold vectorially on the rib...

journal_title：Nature structural & molecular biology

authors： Nilsson OB,Nickson AA,Hollins JJ,Wickles S,Steward A,Beckmann R,von Heijne G,Clarke J

更新日期：2017-03-01 00:00:00

abstract：:Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a speci...

journal_title：Nature structural & molecular biology

authors： Eissenberg JC,Lee MG,Schneider J,Ilvarsonn A,Shiekhattar R,Shilatifard A

更新日期：2007-04-01 00:00:00

abstract：:Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryot...

journal_title：Nature structural & molecular biology

authors： Hu S,Wang X,Shan G

更新日期：2016-11-01 00:00:00

abstract：:p53 binds as a tetramer to DNA targets consisting of two decameric half-sites separated by a variable spacer. Here we present high-resolution crystal structures of complexes between p53 core-domain tetramers and DNA targets consisting of contiguous half-sites. In contrast to previously reported p53-DNA complexes that ...

journal_title：Nature structural & molecular biology

authors： Kitayner M,Rozenberg H,Rohs R,Suad O,Rabinovich D,Honig B,Shakked Z

更新日期：2010-04-01 00:00:00

abstract：:Binding of the gp120 envelope (Env) glycoprotein to the CD4 receptor is the first step in the HIV-1 infectious cycle. Although the CD4-binding site has been extensively characterized, the initial receptor interaction has been difficult to study because of major CD4-induced structural rearrangements. Here we used cryog...

journal_title：Nature structural & molecular biology

authors： Liu Q,Acharya P,Dolan MA,Zhang P,Guzzo C,Lu J,Kwon A,Gururani D,Miao H,Bylund T,Chuang GY,Druz A,Zhou T,Rice WJ,Wigge C,Carragher B,Potter CS,Kwong PD,Lusso P

更新日期：2017-04-01 00:00:00

abstract：:Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with cotranscriptional precursor messenger RNA processing, but little is known about the CTD's function in regulating alternative splicing. We have examined this function using alpha-amanitin-resistant pol II CTD mutants and fibrone...

journal_title：Nature structural & molecular biology

authors： de la Mata M,Kornblihtt AR

更新日期：2006-11-01 00:00:00

abstract：:We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-...

journal_title：Nature structural & molecular biology

authors： Taggart AJ,DeSimone AM,Shih JS,Filloux ME,Fairbrother WG

更新日期：2012-06-17 00:00:00

abstract：:Jun dimerization protein-2 (JDP2) is a component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Here, we examine the functional mechanisms of JDP2 and show that it can inhibit p300-mediated acetylation of core histones in vitro and in vivo. Inhibition of histone...

journal_title：Nature structural & molecular biology

authors： Jin C,Kato K,Chimura T,Yamasaki T,Nakade K,Murata T,Li H,Pan J,Zhao M,Sun K,Chiu R,Ito T,Nagata K,Horikoshi M,Yokoyama KK

更新日期：2006-04-01 00:00:00

abstract：:Integrins are heterodimeric cell-surface adhesion molecules comprising one of 18 possible α-chains and one of eight possible β-chains. They control a range of cell functions in a matrix- and ligand-specific manner. Integrins can be internalized by clathrin-mediated endocytosis (CME) through β subunit-based motifs foun...

journal_title：Nature structural & molecular biology

authors： De Franceschi N,Arjonen A,Elkhatib N,Denessiouk K,Wrobel AG,Wilson TA,Pouwels J,Montagnac G,Owen DJ,Ivaska J

更新日期：2016-02-01 00:00:00

abstract：:Histone modifications are central to the regulation of all DNA-dependent processes. Lys64 of histone H3 (H3K64) lies within the globular domain at a structurally important position. We identify trimethylation of H3K64 (H3K64me3) as a modification that is enriched at pericentric heterochromatin and associated with repe...

journal_title：Nature structural & molecular biology

authors： Daujat S,Weiss T,Mohn F,Lange UC,Ziegler-Birling C,Zeissler U,Lappe M,Schübeler D,Torres-Padilla ME,Schneider R

更新日期：2009-07-01 00:00:00

abstract：:Chaperones, a group of proteins that assist the folding of other proteins, seem to work in a coordinated manner. Two major chaperone families are heat-shock protein families Hsp60 and Hsp70. Here we show for the first time the formation of a stable complex between chaperonin-containing TCP-1 (CCT) and Hsc70, two eukar...

journal_title：Nature structural & molecular biology

authors： Cuéllar J,Martín-Benito J,Scheres SH,Sousa R,Moro F,López-Viñas E,Gómez-Puertas P,Muga A,Carrascosa JL,Valpuesta JM

更新日期：2008-08-01 00:00:00

abstract：:The Rbfox family of splicing factors regulate alternative splicing during animal development and in disease, impacting thousands of exons in the maturing brain, heart and muscle. Rbfox proteins have long been known to bind to the RNA sequence GCAUG with high affinity and specificity, but just half of Rbfox binding sit...

journal_title：Nature structural & molecular biology

authors： Begg BE,Jens M,Wang PY,Minor CM,Burge CB

更新日期：2020-10-01 00:00:00

abstract：:The exon-junction complex (EJC) functionally links splicing to subsequent mRNA localization, translation and stability. Sequence-independent binding of the EJC core to RNA is ensured by the DEAD-box helicase eIF4AIII. Here, we identified the splicing factor CWC22 as a new eIF4AIII partner in flies and humans. CWC22 co...

journal_title：Nature structural & molecular biology

authors： Barbosa I,Haque N,Fiorini F,Barrandon C,Tomasetto C,Blanchette M,Le Hir H

更新日期：2012-10-01 00:00:00

abstract：:Despite growing appreciation of the importance of long noncoding RNAs (lncRNAs) in normal physiology and disease, our knowledge of cancer-related lncRNAs remains limited. By repurposing microarray probes, we constructed expression profiles of 10,207 lncRNA genes in approximately 1,300 tumors over four different cancer...

journal_title：Nature structural & molecular biology

authors： Du Z,Fei T,Verhaak RG,Su Z,Zhang Y,Brown M,Chen Y,Liu XS

更新日期：2013-07-01 00:00:00

abstract：:The ≥ 10³⁰ bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanis...

journal_title：Nature structural & molecular biology

authors： Blower TR,Pei XY,Short FL,Fineran PC,Humphreys DP,Luisi BF,Salmond GP

更新日期：2011-02-01 00:00:00

abstract：:Expansion of CAG/CTG trinucleotide repeats is associated with certain familial neurological disorders, including Huntington's disease. Increasing evidence suggests that formation of a stable DNA hairpin within CAG/CTG repeats during DNA metabolism contributes to their expansion. However, the molecular mechanism(s) by ...

journal_title：Nature structural & molecular biology

authors： Hou C,Chan NL,Gu L,Li GM

更新日期：2009-08-01 00:00:00

abstract：:Primary microRNA cleavage by the Drosha-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the au...

journal_title：Nature structural & molecular biology

authors： Barad O,Mann M,Chapnik E,Shenoy A,Blelloch R,Barkai N,Hornstein E

更新日期：2012-05-13 00:00:00

abstract：:Coupling between transcription and RNA processing is a key gene regulatory mechanism. Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation of the precursor mRNA (pre-mRNA) splicing factors hnRNP A1, U2AF65 and U1 and U5 snRNPs on the intron-containing human FOS gene. These factors w...

journal_title：Nature structural & molecular biology

authors： Listerman I,Sapra AK,Neugebauer KM

更新日期：2006-09-01 00:00:00

abstract：:DNA ligases finalize DNA replication and repair through DNA nick-sealing reactions that can abort to generate cytotoxic 5'-adenylation DNA damage. Aprataxin (Aptx) catalyzes direct reversal of 5'-adenylate adducts to protect genome integrity. Here the structure of a Schizosaccharomyces pombe Aptx-DNA-AMP-Zn(2+) comple...

journal_title：Nature structural & molecular biology

authors： Tumbale P,Appel CD,Kraehenbuehl R,Robertson PD,Williams JS,Krahn J,Ahel I,Williams RS

更新日期：2011-10-09 00:00:00

abstract：:Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryot...

journal_title：Nature structural & molecular biology

authors： Losey HC,Ruthenburg AJ,Verdine GL

更新日期：2006-02-01 00:00:00

abstract：:Histone H3 Lys4 (H3K4) methylation is a prevalent mark associated with transcription activation. A common feature of several H3K4 methyltransferase complexes is the presence of three structural components (RbBP5, Ash2L and WDR5) and a catalytic subunit containing a SET domain. Here we report the first biochemical reco...

journal_title：Nature structural & molecular biology

authors： Dou Y,Milne TA,Ruthenburg AJ,Lee S,Lee JW,Verdine GL,Allis CD,Roeder RG

更新日期：2006-08-01 00:00:00

abstract：:Microtubules are regulated by post-translational modifications of tubulin. The ligation and cleavage of the carboxy-terminal tyrosine of α-tubulin impact microtubule functions during mitosis, cardiomyocyte contraction and neuronal processes. Tubulin tyrosination and detyrosination are mediated by tubulin tyrosine liga...

journal_title：Nature structural & molecular biology

authors： Li F,Hu Y,Qi S,Luo X,Yu H

更新日期：2019-07-01 00:00:00

abstract：:Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of known 3D structures for these enzymes, their mechanism of action has remained poorly understood. Here we report the X-...

journal_title：Nature structural & molecular biology

authors： Zehr E,Szyk A,Piszczek G,Szczesna E,Zuo X,Roll-Mecak A

更新日期：2017-09-01 00:00:00

abstract：:Processing of microRNAs (miRNAs) from their precursors to their biologically active mature forms is regulated during development and cancer. We show that mouse pri- or pre-miR-151 can bind to and compete with mature miR-151-5p and miR-151-3p for binding sites contained within the complementary regions of the E2f6 mRNA...

journal_title：Nature structural & molecular biology

authors： Roy-Chaudhuri B,Valdmanis PN,Zhang Y,Wang Q,Luo QJ,Kay MA

更新日期：2014-09-01 00:00:00