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Insertion of an Alu element in a lncRNA leads to primate-specific modulation of alternative splicing.

Abstract：

:Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryotes including fission yeast and mammals. 5S-OT plays a cis role in regulating the transcription of 5S rRNA in mice and humans. In the anthropoidea suborder of primates, an antisense Alu element has been inserted at the 5S-OT locus. We found that in human cells, 5S-OT regulates alternative splicing of multiple genes in trans via Alu/anti-Alu pairing with target genes and by interacting with the splicing factor U2AF65. This trans effect of 5S-OT in splicing might be exploited in biotechnological applications.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Hu S,Wang X,Shan G

doi

10.1038/nsmb.3302

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

1011-1019

issue

11

eissn

1545-9993

issn

1545-9985

pii

nsmb.3302

journal_volume

23

pub_type

杂志文章

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