Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing.

abstract：:Core Factor (CF) is a conserved RNA polymerase (Pol) I general transcription factor comprising Rrn6, Rrn11 and the TFIIB-related subunit Rrn7. CF binds TATA-binding protein (TBP), Pol I and the regulatory factors Rrn3 and upstream activation factor. We used chemical cross-linking-MS to determine the molecular architec...

journal_title：Nature structural & molecular biology

authors： Knutson BA,Luo J,Ranish J,Hahn S

更新日期：2014-09-01 00:00:00

abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

journal_title：Nature structural & molecular biology

authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

更新日期：2012-06-24 00:00:00

abstract：:Biogenesis of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits, requires assembly chaperones. Here we analyzed the role of Rubisco accumulation factor1 (Raf1), a dimer of ∼40-kDa subunits. We find that Raf1 from Synechococcus elongatus acts downstream of chaperonin-ass...

journal_title：Nature structural & molecular biology

authors： Hauser T,Bhat JY,Miličić G,Wendler P,Hartl FU,Bracher A,Hayer-Hartl M

更新日期：2015-09-01 00:00:00

abstract：:In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the ...

journal_title：Nature structural & molecular biology

authors： Yoshizawa S,Rasubala L,Ose T,Kohda D,Fourmy D,Maenaka K

更新日期：2005-02-01 00:00:00

abstract：:The ability of barrier-to-autointegration factor (BAF) to bind and bridge DNA in a sequence-independent manner is crucial for its role in retroviral integration and a variety of cellular processes. To better understand this behavior, we solved the crystal structure of BAF bound to DNA. The structure reveals that BAF b...

journal_title：Nature structural & molecular biology

authors： Bradley CM,Ronning DR,Ghirlando R,Craigie R,Dyda F

更新日期：2005-10-01 00:00:00

abstract：:Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall qua...

journal_title：Nature structural & molecular biology

authors： Gautier A,Mott HR,Bostock MJ,Kirkpatrick JP,Nietlispach D

更新日期：2010-06-01 00:00:00

abstract：:Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal struc...

journal_title：Nature structural & molecular biology

authors： Huang J,Brown AF,Wu J,Xue J,Bley CJ,Rand DP,Wu L,Zhang R,Chen JJ,Lei M

更新日期：2014-06-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) play critical roles during tumorigenesis by functioning as scaffolds that regulate protein-protein, protein-DNA or protein-RNA interactions. Using a clinically guided genetic screening approach, we identified lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1), which is overexpre...

journal_title：Nature structural & molecular biology

authors： Zhang Y,He Q,Hu Z,Feng Y,Fan L,Tang Z,Yuan J,Shan W,Li C,Hu X,Tanyi JL,Fan Y,Huang Q,Montone K,Dang CV,Zhang L

更新日期：2016-06-01 00:00:00

abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

journal_title：Nature structural & molecular biology

authors： Vincent JA,Kwong TJ,Tsukiyama T

更新日期：2008-05-01 00:00:00

abstract：:A fundamental question is how enzymes can accelerate chemical reactions. Catalysis is not only defined by actual chemical steps, but also by enzyme structure and dynamics. To investigate the role of protein dynamics in enzymatic turnover, we measured residue-specific protein dynamics in hyperthermophilic and mesophili...

journal_title：Nature structural & molecular biology

authors： Wolf-Watz M,Thai V,Henzler-Wildman K,Hadjipavlou G,Eisenmesser EZ,Kern D

更新日期：2004-10-01 00:00:00

abstract：:Myotonic dystrophy is an RNA gain-of-function disease caused by expanded CUG or CCUG repeats, which sequester the RNA binding protein MBNL1. Here we describe a newly discovered function for MBNL1 as a regulator of pre-miR-1 biogenesis and find that miR-1 processing is altered in heart samples from people with myotonic...

journal_title：Nature structural & molecular biology

authors： Rau F,Freyermuth F,Fugier C,Villemin JP,Fischer MC,Jost B,Dembele D,Gourdon G,Nicole A,Duboc D,Wahbi K,Day JW,Fujimura H,Takahashi MP,Auboeuf D,Dreumont N,Furling D,Charlet-Berguerand N

更新日期：2011-06-19 00:00:00

abstract：:Ubiquitination is a post-translational modification (PTM) that is essential for balancing numerous physiological processes. To enable delineation of protein ubiquitination at a site-specific level, we generated an antibody, denoted UbiSite, recognizing the C-terminal 13 amino acids of ubiquitin, which remain attached ...

journal_title：Nature structural & molecular biology

authors： Akimov V,Barrio-Hernandez I,Hansen SVF,Hallenborg P,Pedersen AK,Bekker-Jensen DB,Puglia M,Christensen SDK,Vanselow JT,Nielsen MM,Kratchmarova I,Kelstrup CD,Olsen JV,Blagoev B

更新日期：2018-07-01 00:00:00

abstract：:The choice of codons can influence local translation kinetics during protein synthesis. Whether codon preference is linked to cotranslational regulation of polypeptide folding remains unclear. Here, we derive a revised translational efficiency scale that incorporates the competition between tRNA supply and demand. App...

journal_title：Nature structural & molecular biology

authors： Pechmann S,Frydman J

更新日期：2013-02-01 00:00:00

abstract：:Many aspects of nucleic acid metabolism, such as DNA replication, repair and transcription, are regulated by the post-translational modifiers ubiquitin and SUMO. Not surprisingly, DNA itself plays an integral part in determining the modification of most chromatin-associated targets. Conversely, ubiquitination or SUMOy...

journal_title：Nature structural & molecular biology

authors： Ulrich HD

更新日期：2014-04-01 00:00:00

abstract：:Histone H3 Lys4 (H3K4) methylation is a prevalent mark associated with transcription activation. A common feature of several H3K4 methyltransferase complexes is the presence of three structural components (RbBP5, Ash2L and WDR5) and a catalytic subunit containing a SET domain. Here we report the first biochemical reco...

journal_title：Nature structural & molecular biology

authors： Dou Y,Milne TA,Ruthenburg AJ,Lee S,Lee JW,Verdine GL,Allis CD,Roeder RG

更新日期：2006-08-01 00:00:00

abstract：:Processing of transcribed precursor ribosomal RNA (pre-rRNA) to a mature state is a conserved aspect of ribosome biogenesis in vivo. We developed an affinity-purification system to isolate and analyze in vivo-formed pre-rRNA-containing ribonucleoprotein (RNP) particles (rRNPs) from wild-type E. coli. We observed that ...

journal_title：Nature structural & molecular biology

authors： Gupta N,Culver GM

更新日期：2014-10-01 00:00:00

abstract：:We describe a novel approach to separate two ribosome populations from the same cells and use this method in combination with RNA-seq to identify mRNAs bound to Saccharomyces cerevisiae ribosomes with and without Rps26, a protein linked to the pathogenesis of Diamond-Blackfan anemia (DBA). These analyses reveal that R...

journal_title：Nature structural & molecular biology

authors： Ferretti MB,Ghalei H,Ward EA,Potts EL,Karbstein K

更新日期：2017-09-01 00:00:00

abstract：:Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by c...

journal_title：Nature structural & molecular biology

authors： Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

更新日期：2006-08-01 00:00:00

abstract：:In budding yeast, DNA damage in G1 activates a Rad9-dependent checkpoint that targets the cyclin-dependent kinase (CDK) Cdc28 to delay G1 exit. After a transient arrest, cells may enter S phase before completing DNA repair. We used genetic analysis to identify the stress-responsive CDK Pho85, the cyclin Pho80 and the ...

journal_title：Nature structural & molecular biology

authors： Wysocki R,Javaheri A,Kristjansdottir K,Sha F,Kron SJ

更新日期：2006-10-01 00:00:00

abstract：:The DNA-damage response (DDR) ensures genome stability and proper inheritance of genetic information, both of which are essential to survival. It is presently unclear to what extent other signaling pathways modulate DDR function. Here we show that Notch receptor binds and inactivates ATM kinase and that this mechanism...

journal_title：Nature structural & molecular biology

authors： Vermezovic J,Adamowicz M,Santarpia L,Rustighi A,Forcato M,Lucano C,Massimiliano L,Costanzo V,Bicciato S,Del Sal G,d'Adda di Fagagna F

更新日期：2015-05-01 00:00:00

abstract：:The Ca(2+) sensor synaptotagmin-1 is thought to trigger membrane fusion by binding to acidic membrane lipids and SNARE proteins. Previous work has shown that binding is mediated by electrostatic interactions that are sensitive to the ionic environment. However, the influence of divalent or polyvalent ions, at physiolo...

journal_title：Nature structural & molecular biology

authors： Park Y,Seo JB,Fraind A,Pérez-Lara A,Yavuz H,Han K,Jung SR,Kattan I,Walla PJ,Choi M,Cafiso DS,Koh DS,Jahn R

更新日期：2015-10-01 00:00:00

abstract：:Flavin-containing monooxygenases (FMOs) are ubiquitous in all domains of life and metabolize a myriad of xenobiotics, including toxins, pesticides and drugs. However, despite their pharmacological importance, structural information remains bereft. To further our understanding behind their biochemistry and diversity, w...

journal_title：Nature structural & molecular biology

authors： Nicoll CR,Bailleul G,Fiorentini F,Mascotti ML,Fraaije MW,Mattevi A

更新日期：2020-01-01 00:00:00

abstract：:Aggregation of proteins containing polyglutamine (polyQ) expansions characterizes many neurodegenerative disorders, including Huntington's disease. Molecular chaperones modulate the aggregation and toxicity of the huntingtin (Htt) protein by an ill-defined mechanism. Here we determine how the chaperonin TRiC suppresse...

journal_title：Nature structural & molecular biology

authors： Tam S,Spiess C,Auyeung W,Joachimiak L,Chen B,Poirier MA,Frydman J

更新日期：2009-12-01 00:00:00

abstract：:Allosteric interactions are typically considered to proceed through a series of discrete changes in bonding interactions that alter the protein conformation. Here we show that allostery can be mediated exclusively by transmitted changes in protein motions. We have characterized the negatively cooperative binding of cA...

journal_title：Nature structural & molecular biology

authors： Popovych N,Sun S,Ebright RH,Kalodimos CG

更新日期：2006-09-01 00:00:00

abstract：:Microtubules are regulated by post-translational modifications of tubulin. The ligation and cleavage of the carboxy-terminal tyrosine of α-tubulin impact microtubule functions during mitosis, cardiomyocyte contraction and neuronal processes. Tubulin tyrosination and detyrosination are mediated by tubulin tyrosine liga...

journal_title：Nature structural & molecular biology

authors： Li F,Hu Y,Qi S,Luo X,Yu H

更新日期：2019-07-01 00:00:00

abstract：:BAK and BAX are essential mediators of apoptosis that oligomerize in response to death cues, thereby causing permeabilization of the mitochondrial outer membrane. Their transition from quiescent monomers to pore-forming oligomers involves a well-characterized symmetric dimer intermediate. However, no essential seconda...

journal_title：Nature structural & molecular biology

authors： Cowan AD,Smith NA,Sandow JJ,Kapp EA,Rustam YH,Murphy JM,Brouwer JM,Bernardini JP,Roy MJ,Wardak AZ,Tan IK,Webb AI,Gulbis JM,Smith BJ,Reid GE,Dewson G,Colman PM,Czabotar PE

更新日期：2020-11-01 00:00:00

abstract：:Although both homologous recombination (HR) and nonhomologous end joining can repair DNA double-strand breaks (DSBs), the mechanisms by which one of these pathways is chosen over the other remain unclear. Here we show that transcriptionally active chromatin is preferentially repaired by HR. Using chromatin immunopreci...

journal_title：Nature structural & molecular biology

authors： Aymard F,Bugler B,Schmidt CK,Guillou E,Caron P,Briois S,Iacovoni JS,Daburon V,Miller KM,Jackson SP,Legube G

更新日期：2014-04-01 00:00:00

abstract：:Integrins are heterodimeric cell-surface adhesion molecules comprising one of 18 possible α-chains and one of eight possible β-chains. They control a range of cell functions in a matrix- and ligand-specific manner. Integrins can be internalized by clathrin-mediated endocytosis (CME) through β subunit-based motifs foun...

journal_title：Nature structural & molecular biology

authors： De Franceschi N,Arjonen A,Elkhatib N,Denessiouk K,Wrobel AG,Wilson TA,Pouwels J,Montagnac G,Owen DJ,Ivaska J

更新日期：2016-02-01 00:00:00

abstract：:We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show...

journal_title：Nature structural & molecular biology

authors： Kortemme T,Joachimiak LA,Bullock AN,Schuler AD,Stoddard BL,Baker D

更新日期：2004-04-01 00:00:00

abstract：:Glucokinase (GK) is a glucose-phosphorylating enzyme that regulates insulin release and hepatic metabolism, and its loss of function is implicated in diabetes pathogenesis. GK activators (GKAs) are attractive therapeutics in diabetes; however, clinical data indicate that their benefits can be offset by hypoglycemia, o...

journal_title：Nature structural & molecular biology

authors： Szlyk B,Braun CR,Ljubicic S,Patton E,Bird GH,Osundiji MA,Matschinsky FM,Walensky LD,Danial NN

更新日期：2014-01-01 00:00:00