Abstract:
:A fundamental question is how enzymes can accelerate chemical reactions. Catalysis is not only defined by actual chemical steps, but also by enzyme structure and dynamics. To investigate the role of protein dynamics in enzymatic turnover, we measured residue-specific protein dynamics in hyperthermophilic and mesophilic homologs of adenylate kinase during catalysis. A dynamic process, the opening of the nucleotide-binding lids, was found to be rate-limiting for both enzymes as measured by NMR relaxation. Moreover, we found that the reduced catalytic activity of the hyperthermophilic enzyme at ambient temperatures is caused solely by a slower lid-opening rate. This comparative and quantitative study of activity, structure and dynamics revealed a close link between protein dynamics and catalytic turnover.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Wolf-Watz M,Thai V,Henzler-Wildman K,Hadjipavlou G,Eisenmesser EZ,Kern Ddoi
10.1038/nsmb821keywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
945-9issue
10eissn
1545-9993issn
1545-9985pii
nsmb821journal_volume
11pub_type
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