当前位置： SCI文献检索 > NATURE STRUCTURAL & MOLECULAR BIOLOGY期刊下所有文献 > CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage.

CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage.

Abstract：

:In budding yeast, DNA damage in G1 activates a Rad9-dependent checkpoint that targets the cyclin-dependent kinase (CDK) Cdc28 to delay G1 exit. After a transient arrest, cells may enter S phase before completing DNA repair. We used genetic analysis to identify the stress-responsive CDK Pho85, the cyclin Pho80 and the targeted transcription factors Pho4 and Swi5 as determinants of G1 checkpoint adaptation. Consistent with opposing roles for the Cdc28 inhibitor Sic1 in blocking G1 exit and Pho85 in targeting Sic1 for proteolysis, mutation of Sic1 curtails G1 checkpoint delay, whereas Pho85 inhibition after DNA damage promotes Sic1 stability. G1 checkpoint delay in mutants lacking both Sic1 and Pho4 is independent of Pho85 activity. These data establish a G1 checkpoint adaptation pathway where Pho85 mediates Pho4 downregulation and Sic1 degradation to release Cdc28 activity and promote onset of S phase.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Wysocki R,Javaheri A,Kristjansdottir K,Sha F,Kron SJ

doi

10.1038/nsmb1139

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

908-14

issue

10

eissn

1545-9993

issn

1545-9985

pii

nsmb1139

journal_volume

13

pub_type

杂志文章

相关文献

NATURE STRUCTURAL & MOLECULAR BIOLOGY文献大全
  • A novel tripartite motif involved in aquaporin topogenesis, monomer folding and tetramerization.

    abstract：:Aquaporin (AQP) folding in the endoplasmic reticulum is characterized by two distinct pathways of membrane insertion that arise from divergent residues within the second transmembrane segment. We now show that in AQP1 these residues (Asn49 and Lys51) interact with Asp185 at the C terminus of TM5 to form a polar, quate...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1275

    authors： Buck TM,Wagner J,Grund S,Skach WR

    更新日期：2007-08-01 00:00:00

  • Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA.

    abstract：:Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryot...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1047

    authors： Losey HC,Ruthenburg AJ,Verdine GL

    更新日期：2006-02-01 00:00:00

  • UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites.

    abstract：:Ubiquitination is a post-translational modification (PTM) that is essential for balancing numerous physiological processes. To enable delineation of protein ubiquitination at a site-specific level, we generated an antibody, denoted UbiSite, recognizing the C-terminal 13 amino acids of ubiquitin, which remain attached ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0084-y

    authors： Akimov V,Barrio-Hernandez I,Hansen SVF,Hallenborg P,Pedersen AK,Bekker-Jensen DB,Puglia M,Christensen SDK,Vanselow JT,Nielsen MM,Kratchmarova I,Kelstrup CD,Olsen JV,Blagoev B

    更新日期：2018-07-01 00:00:00

  • The stoichiometry of Gag protein in HIV-1.

    abstract：:The major structural components of HIV-1 are encoded as a single polyprotein, Gag, which is sufficient for virus particle assembly. Initially, Gag forms an approximately spherical shell underlying the membrane of the immature particle. After proteolytic maturation of Gag, the capsid (CA) domain of Gag reforms into a c...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb785

    authors： Briggs JA,Simon MN,Gross I,Kräusslich HG,Fuller SD,Vogt VM,Johnson MC

    更新日期：2004-07-01 00:00:00

  • Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation.

    abstract：:Hsp70s use ATP hydrolysis to disrupt protein-protein associations and to move macromolecules. One example is the Hsc70- mediated disassembly of the clathrin coats that form on vesicles during endocytosis. Here, we exploited the exceptional features of these coats to test three models-Brownian ratchet, power-stroke and...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3272

    authors： Sousa R,Liao HS,Cuéllar J,Jin S,Valpuesta JM,Jin AJ,Lafer EM

    更新日期：2016-09-01 00:00:00

  • Scratching the (lateral) surface of chromatin regulation by histone modifications.

    abstract：:Histones have two structurally and functionally distinct domains: globular domains forming the nucleosomal core around which DNA is wrapped and unstructured tails protruding from the nucleosomal core. Whereas post-translational modifications (PTMs) in histone tails are well studied, much less is currently known about ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2581

    authors： Tropberger P,Schneider R

    更新日期：2013-06-01 00:00:00

  • Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.

    abstract：:Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The eukaryotic SMC complexes cohesin and condensin are thought to fold interphase and mitotic chromosomes, respectively, into large l...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0457-x

    authors： Lee BG,Merkel F,Allegretti M,Hassler M,Cawood C,Lecomte L,O'Reilly FJ,Sinn LR,Gutierrez-Escribano P,Kschonsak M,Bravo S,Nakane T,Rappsilber J,Aragon L,Beck M,Löwe J,Haering CH

    更新日期：2020-08-01 00:00:00

  • Structures of human DNA polymerases ν and θ expose their end game.

    abstract：:Although the two B-family human DNA polymerases, pol δ and pol ε, are responsible for the bulk of nuclear genome replication, at least 14 additional polymerases have roles in nuclear DNA repair and replication. In this issue, newly reported crystal structures of two specialized A-family polymerases, pol δ and pol ε, e...

    journal_title：Nature structural & molecular biology

    pub_type： 评论,杂志文章

    doi：10.1038/nsmb.3006

    authors： Beard WA,Wilson SH

    更新日期：2015-04-01 00:00:00

  • Hybrid molecular structure of the giant protease tripeptidyl peptidase II.

    abstract：:Tripeptidyl peptidase II (TPP II) is the largest known eukaryotic protease (6 MDa). It is believed to act downstream of the 26S proteasome, cleaving tripeptides from the N termini of longer peptides, and it is implicated in numerous cellular processes. Here we report the structure of Drosophila TPP II determined by a ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1870

    authors： Chuang CK,Rockel B,Seyit G,Walian PJ,Schönegge AM,Peters J,Zwart PH,Baumeister W,Jap BK

    更新日期：2010-08-01 00:00:00

  • Katanin spiral and ring structures shed light on power stroke for microtubule severing.

    abstract：:Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of known 3D structures for these enzymes, their mechanism of action has remained poorly understood. Here we report the X-...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3448

    authors： Zehr E,Szyk A,Piszczek G,Szczesna E,Zuo X,Roll-Mecak A

    更新日期：2017-09-01 00:00:00

  • An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome.

    abstract：:Simultaneous profiling of transcriptome and chromatin accessibility within single cells is a powerful approach to dissect gene regulatory programs in complex tissues. However, current tools are limited by modest throughput. We now describe an ultra high-throughput method, Paired-seq, for parallel analysis of transcrip...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-019-0323-x

    authors： Zhu C,Yu M,Huang H,Juric I,Abnousi A,Hu R,Lucero J,Behrens MM,Hu M,Ren B

    更新日期：2019-11-01 00:00:00

  • Linkage between dynamics and catalysis in a thermophilic-mesophilic enzyme pair.

    abstract：:A fundamental question is how enzymes can accelerate chemical reactions. Catalysis is not only defined by actual chemical steps, but also by enzyme structure and dynamics. To investigate the role of protein dynamics in enzymatic turnover, we measured residue-specific protein dynamics in hyperthermophilic and mesophili...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb821

    authors： Wolf-Watz M,Thai V,Henzler-Wildman K,Hadjipavlou G,Eisenmesser EZ,Kern D

    更新日期：2004-10-01 00:00:00

  • Location, location, location: a crystal-clear view of autotaxin saturating LPA receptors.

    abstract：:The interaction of autotaxin with its substrates leads to the production of lysophosphatidic acids (LPA), bioactive lipids with an emerging prominent role in inflammation and cancer. Two papers in this issue tell the previously unknown story of autotaxin, from substrate discrimination to highly efficient local deliver...

    journal_title：Nature structural & molecular biology

    pub_type： 评论,杂志文章

    doi：10.1038/nsmb0211-117

    authors： Tabchy A,Tigyi G,Mills GB

    更新日期：2011-02-01 00:00:00

  • A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses.

    abstract：:In nutrient-starved bacteria, RelA and SpoT proteins have key roles in reducing cell growth and overcoming stresses. Here we identify functional SpoT orthologs in metazoa (named Mesh1, encoded by HDDC3 in human and Q9VAM9 in Drosophila melanogaster) and reveal their structures and functions. Like the bacterial enzyme,...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1906

    authors： Sun D,Lee G,Lee JH,Kim HY,Rhee HW,Park SY,Kim KJ,Kim Y,Kim BY,Hong JI,Park C,Choy HE,Kim JH,Jeon YH,Chung J

    更新日期：2010-10-01 00:00:00

  • Evidence for a group II intron-like catalytic triplex in the spliceosome.

    abstract：:To catalyze pre-mRNA splicing, U6 small nuclear RNA positions two metals that interact directly with the scissile phosphates. U6 metal ligands correspond stereospecifically to metal ligands within the catalytic domain V of a group II self-splicing intron. Domain V ligands are organized by base-triple interactions, whi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2815

    authors： Fica SM,Mefford MA,Piccirilli JA,Staley JP

    更新日期：2014-05-01 00:00:00

  • RNA secondary structure in mutually exclusive splicing.

    abstract：:Mutually exclusive splicing is a regulated means to generate protein diversity, but the underlying mechanisms are poorly understood. Here comparative genome analysis revealed the built-in intronic elements for controlling mutually exclusive splicing of the 14-3-3ξ pre-mRNA. These elements are clade specific but are ev...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1959

    authors： Yang Y,Zhan L,Zhang W,Sun F,Wang W,Tian N,Bi J,Wang H,Shi D,Jiang Y,Zhang Y,Jin Y

    更新日期：2011-02-01 00:00:00

  • Xist-dependent imprinted X inactivation and the early developmental consequences of its failure.

    abstract：:The long noncoding RNA Xist is expressed from only the paternal X chromosome in mouse preimplantation female embryos and mediates transcriptional silencing of that chromosome. In females, absence of Xist leads to postimplantation lethality. Here, through single-cell RNA sequencing of early preimplantation mouse embryo...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3365

    authors： Borensztein M,Syx L,Ancelin K,Diabangouaya P,Picard C,Liu T,Liang JB,Vassilev I,Galupa R,Servant N,Barillot E,Surani A,Chen CJ,Heard E

    更新日期：2017-03-01 00:00:00

  • Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d.

    abstract：:The tandem zinc finger (TZF) domain of the protein TIS11d binds to the class II AU-rich element (ARE) in the 3' untranslated region (3' UTR) of target mRNAs and promotes their deadenylation and degradation. The NMR structure of the TIS11d TZF domain bound to the RNA sequence 5'-UUAUUUAUU-3' comprises a pair of novel C...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb738

    authors： Hudson BP,Martinez-Yamout MA,Dyson HJ,Wright PE

    更新日期：2004-03-01 00:00:00

  • Cryo-EM structure of a human prion fibril with a hydrophobic, protease-resistant core.

    abstract：:Self-templating assemblies of the human prion protein are clinically associated with transmissible spongiform encephalopathies. Here we present the cryo-EM structure of a denaturant- and protease-resistant fibril formed in vitro spontaneously by a 9.7-kDa unglycosylated fragment of the human prion protein. This human ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0403-y

    authors： Glynn C,Sawaya MR,Ge P,Gallagher-Jones M,Short CW,Bowman R,Apostol M,Zhou ZH,Eisenberg DS,Rodriguez JA

    更新日期：2020-05-01 00:00:00

  • A newly discovered function for RNase L in regulating translation termination.

    abstract：:The antiviral and antiproliferative effects of interferons are mediated in part by the 2'-5' oligoadenylate-RNase L RNA decay pathway. RNase L is an endoribonuclease that requires 2'-5' oligoadenylates to cleave single-stranded RNA. In this report we present evidence demonstrating a role for RNase L in translation. We...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb944

    authors： Le Roy F,Salehzada T,Bisbal C,Dougherty JP,Peltz SW

    更新日期：2005-06-01 00:00:00

  • Structural insights into RNA processing by the human RISC-loading complex.

    abstract：:Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC). In humans, Argonaute2 (AGO2) assembles with the guide RNA-generating enzyme Di...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1673

    authors： Wang HW,Noland C,Siridechadilok B,Taylor DW,Ma E,Felderer K,Doudna JA,Nogales E

    更新日期：2009-11-01 00:00:00

  • Structure of a purine-purine wobble base pair in the decoding center of the ribosome.

    abstract：:Here we report the crystal structures of I.C and I.A wobble base pairs in the context of the ribosomal decoding center, clearly showing that the I.A base pair is of an I(anti).A(anti) conformation, as predicted by Crick. Additionally, the structures enable the observation of changes in the anticodon to allow purine-pu...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb866

    authors： Murphy FV 4th,Ramakrishnan V

    更新日期：2004-12-01 00:00:00

  • Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2.

    abstract：:Brr2 is a DExD/H-box helicase responsible for U4/U6 unwinding during spliceosomal activation. Brr2 contains two helicase-like domains, each of which is followed by a Sec63 domain with unknown function. We determined the crystal structure of the second Sec63 domain, which unexpectedly resembles domains 4 and 5 of DNA h...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1625

    authors： Zhang L,Xu T,Maeder C,Bud LO,Shanks J,Nix J,Guthrie C,Pleiss JA,Zhao R

    更新日期：2009-07-01 00:00:00

  • Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL.

    abstract：:Herpesviruses, which cause many incurable diseases, infect cells by fusing viral and cellular membranes. Whereas most other enveloped viruses use a single viral catalyst called a fusogen, herpesviruses, inexplicably, require two conserved fusion-machinery components, gB and the heterodimer gH-gL, plus other nonconserv...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1837

    authors： Chowdary TK,Cairns TM,Atanasiu D,Cohen GH,Eisenberg RJ,Heldwein EE

    更新日期：2010-07-01 00:00:00

  • Fast ribozyme cleavage releases transcripts from RNA polymerase II and aborts co-transcriptional pre-mRNA processing.

    abstract：:We investigated whether a continuous transcript is necessary for co-transcriptional pre-mRNA processing. Cutting an intron with the fast-cleaving hepatitis delta ribozyme, but not the slower hammerhead, inhibited splicing. Therefore, exon tethering to RNA polymerase II (Pol II) cannot rescue splicing of a transcript s...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1652

    authors： Fong N,Ohman M,Bentley DL

    更新日期：2009-09-01 00:00:00

  • Multiple in vivo pathways for Escherichia coli small ribosomal subunit assembly occur on one pre-rRNA.

    abstract：:Processing of transcribed precursor ribosomal RNA (pre-rRNA) to a mature state is a conserved aspect of ribosome biogenesis in vivo. We developed an affinity-purification system to isolate and analyze in vivo-formed pre-rRNA-containing ribonucleoprotein (RNP) particles (rRNPs) from wild-type E. coli. We observed that ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2887

    authors： Gupta N,Culver GM

    更新日期：2014-10-01 00:00:00

  • Synaptotagmin arrests the SNARE complex before triggering fast, efficient membrane fusion in response to Ca2+.

    abstract：:Neuronal communication is mediated by Ca(2+)-triggered fusion of transmitter-filled synaptic vesicles with the presynaptic plasma membrane. Synaptotagmin I functions as a Ca(2+) sensor that regulates exocytosis, whereas soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins in th...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1463

    authors： Chicka MC,Hui E,Liu H,Chapman ER

    更新日期：2008-08-01 00:00:00

  • Catching DNA with hoops-biophysical approaches to clarify the mechanism of SMC proteins.

    abstract：:Structural maintenance of chromosome (SMC) complexes are central regulators of chromosome architecture that are essential in all domains of life. For decades, the structural biology field has been debating how these conserved protein complexes use their intricate ring-like structures to structurally organize DNA. Here...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb.3507

    authors： Eeftens J,Dekker C

    更新日期：2017-12-07 00:00:00

  • An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation.

    abstract：:Many viruses bypass canonical cap-dependent translation in host cells by using internal ribosomal entry sites (IRESs) in their transcripts; IRESs hijack initiation factors for the assembly of initiation complexes. However, it is currently unknown how IRES RNAs recognize initiation factors that have no endogenous RNA b...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3280

    authors： Imai S,Kumar P,Hellen CU,D'Souza VM,Wagner G

    更新日期：2016-09-01 00:00:00

  • Ribosome-stalk biogenesis is coupled with recruitment of nuclear-export factor to the nascent 60S subunit.

    abstract：:Nuclear export of preribosomal subunits is a key step during eukaryotic ribosome formation. To efficiently pass through the FG-repeat meshwork of the nuclear pore complex, the large pre-60S subunit requires several export factors. Here we describe the mechanism of recruitment of the Saccharomyces cerevisiae RNA-export...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3312

    authors： Sarkar A,Pech M,Thoms M,Beckmann R,Hurt E

    更新日期：2016-12-01 00:00:00