Abstract:
:Aquaporin (AQP) folding in the endoplasmic reticulum is characterized by two distinct pathways of membrane insertion that arise from divergent residues within the second transmembrane segment. We now show that in AQP1 these residues (Asn49 and Lys51) interact with Asp185 at the C terminus of TM5 to form a polar, quaternary structural motif that influences multiple stages of folding. Asn49 and Asp185 form an intramolecular hydrogen bond needed for proper helical packing, monomer formation and function. In contrast, Lys51 interacts with Asp185 on an adjacent monomer to stabilize the AQP1 tetramer. Although these residues are unique to AQP1, they share a highly conserved architecture whose functional properties can be transferred to other family members. These findings suggest a general mechanism by which evolutionary divergence of membrane proteins can confer new functional properties via alternative folding pathways that give rise to a common final structure.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Buck TM,Wagner J,Grund S,Skach WRdoi
10.1038/nsmb1275subject
Has Abstractpub_date
2007-08-01 00:00:00pages
762-9issue
8eissn
1545-9993issn
1545-9985pii
nsmb1275journal_volume
14pub_type
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