当前位置： SCI文献检索 > NATURE STRUCTURAL & MOLECULAR BIOLOGY期刊下所有文献 > The stoichiometry of Gag protein in HIV-1.

The stoichiometry of Gag protein in HIV-1.

Abstract：

:The major structural components of HIV-1 are encoded as a single polyprotein, Gag, which is sufficient for virus particle assembly. Initially, Gag forms an approximately spherical shell underlying the membrane of the immature particle. After proteolytic maturation of Gag, the capsid (CA) domain of Gag reforms into a conical shell enclosing the RNA genome. This mature shell contains 1,000-1,500 CA proteins assembled into a hexameric lattice with a spacing of 10 nm. By contrast, little is known about the structure of the immature virus. We used cryo-EM and scanning transmission EM to determine that an average (145 nm diameter) complete immature HIV particle contains approximately 5,000 structural (Gag) proteins, more than twice the number from previous estimates. In the immature virus, Gag forms a hexameric lattice with a spacing of 8.0 nm. Thus, less than half of the CA proteins form the mature core.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Briggs JA,Simon MN,Gross I,Kräusslich HG,Fuller SD,Vogt VM,Johnson MC

doi

10.1038/nsmb785

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

672-5

issue

7

eissn

1545-9993

issn

1545-9985

pii

nsmb785

journal_volume

11

pub_type

杂志文章

相关文献

NATURE STRUCTURAL & MOLECULAR BIOLOGY文献大全
  • Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer.

    abstract：:Long noncoding RNAs (lncRNAs) play critical roles during tumorigenesis by functioning as scaffolds that regulate protein-protein, protein-DNA or protein-RNA interactions. Using a clinically guided genetic screening approach, we identified lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1), which is overexpre...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3211

    authors： Zhang Y,He Q,Hu Z,Feng Y,Fan L,Tang Z,Yuan J,Shan W,Li C,Hu X,Tanyi JL,Fan Y,Huang Q,Montone K,Dang CV,Zhang L

    更新日期：2016-06-01 00:00:00

  • Hemifusion arrest by complexin is relieved by Ca2+-synaptotagmin I.

    abstract：:Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by c...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1124

    authors： Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

    更新日期：2006-08-01 00:00:00

  • Publisher Correction: Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature structural & molecular biology

    pub_type： 已发布勘误

    doi：10.1038/s41594-020-0378-8

    authors： Nicoll CR,Bailleul G,Fiorentini F,Mascotti ML,Fraaije MW,Mattevi A

    更新日期：2020-02-01 00:00:00

  • BRD4 assists elongation of both coding and enhancer RNAs by interacting with acetylated histones.

    abstract：:Small-molecule BET inhibitors interfere with the epigenetic interactions between acetylated histones and the bromodomains of the BET family proteins, including BRD4, and they potently inhibit growth of malignant cells by targeting cancer-promoting genes. BRD4 interacts with the pause-release factor P-TEFb and has been...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2912

    authors： Kanno T,Kanno Y,LeRoy G,Campos E,Sun HW,Brooks SR,Vahedi G,Heightman TD,Garcia BA,Reinberg D,Siebenlist U,O'Shea JJ,Ozato K

    更新日期：2014-12-01 00:00:00

  • Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region.

    abstract：:The monoclonal antibody 13H11 shares part of its epitope in the HIV-1 gp41 membrane-proximal external region (MPER) with the rare, broadly neutralizing human antibody 2F5. Although 13H11 partially cross-blocked 2F5 binding, 13H11 is non-neutralizing and does not block 2F5 neutralization. We show that unlike 2F5, 13H11...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1944

    authors： Nicely NI,Dennison SM,Spicer L,Scearce RM,Kelsoe G,Ueda Y,Chen H,Liao HX,Alam SM,Haynes BF

    更新日期：2010-12-01 00:00:00

  • A conserved structural motif mediates formation of the periplasmic rings in the type III secretion system.

    abstract：:The type III secretion system (T3SS) is a macromolecular 'injectisome' that allows bacterial pathogens to transport virulence proteins into the eukaryotic host cell. This macromolecular complex is composed of connected ring-like structures that span both bacterial membranes. The crystal structures of the periplasmic d...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1603

    authors： Spreter T,Yip CK,Sanowar S,André I,Kimbrough TG,Vuckovic M,Pfuetzner RA,Deng W,Yu AC,Finlay BB,Baker D,Miller SI,Strynadka NC

    更新日期：2009-05-01 00:00:00

  • RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.

    abstract：:The RNA polymerase II (RNAPII) C-terminal domain (CTD) heptapeptide repeats (1-YSPTSPS-7) undergo dynamic phosphorylation and dephosphorylation during the transcription cycle to recruit factors that regulate transcription, RNA processing and chromatin modification. We show here that RPRD1A and RPRD1B form homodimers a...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2853

    authors： Ni Z,Xu C,Guo X,Hunter GO,Kuznetsova OV,Tempel W,Marcon E,Zhong G,Guo H,Kuo WW,Li J,Young P,Olsen JB,Wan C,Loppnau P,El Bakkouri M,Senisterra GA,He H,Huang H,Sidhu SS,Emili A,Murphy S,Mosley AL,Arrowsmith CH

    更新日期：2014-08-01 00:00:00

  • Structural basis for selective activation of ABA receptors.

    abstract：:Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discov...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1898

    authors： Peterson FC,Burgie ES,Park SY,Jensen DR,Weiner JJ,Bingman CA,Chang CE,Cutler SR,Phillips GN Jr,Volkman BF

    更新日期：2010-09-01 00:00:00

  • Substrate-engaged 26S proteasome.

    abstract：: ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0167-9

    authors： Marcinkiewicz KM

    更新日期：2018-12-01 00:00:00

  • Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.

    abstract：:In response to stress, cells attenuate global protein synthesis but permit efficient translation of mRNAs encoding heat-shock proteins (HSPs). Although decades have passed since the first description of the heat-shock response, how cells achieve translational control of HSP synthesis remains enigmatic. Here we report ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3010

    authors： Zhang X,Gao X,Coots RA,Conn CS,Liu B,Qian SB

    更新日期：2015-05-01 00:00:00

  • Binding-induced folding of prokaryotic ubiquitin-like protein on the Mycobacterium proteasomal ATPase targets substrates for degradation.

    abstract：:Mycobacterium tuberculosis uses a proteasome system that is analogous to the eukaryotic ubiquitin-proteasome pathway and is required for pathogenesis. However, the bacterial analog of ubiquitin, prokaryotic ubiquitin-like protein (Pup), is an intrinsically disordered protein that bears little sequence or structural re...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1918

    authors： Wang T,Darwin KH,Li H

    更新日期：2010-11-01 00:00:00

  • Chromatin organization marks exon-intron structure.

    abstract：:An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from h...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1659

    authors： Schwartz S,Meshorer E,Ast G

    更新日期：2009-09-01 00:00:00

  • RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1.

    abstract：:Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by degradation of nascent peptides. However, endogenous RQC-inducing sequences and the mechanism underlying the...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0393-9

    authors： Matsuo Y,Tesina P,Nakajima S,Mizuno M,Endo A,Buschauer R,Cheng J,Shounai O,Ikeuchi K,Saeki Y,Becker T,Beckmann R,Inada T

    更新日期：2020-04-01 00:00:00

  • Defining the landscape of ATP-competitive inhibitor resistance residues in protein kinases.

    abstract：:Kinases are involved in disease development and modulation of their activity can be therapeutically beneficial. Drug-resistant mutant kinases are valuable tools in drug discovery efforts, but the prediction of mutants across the kinome is challenging. Here, we generate deep mutational scanning data to identify mutant ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-019-0358-z

    authors： Persky NS,Hernandez D,Do Carmo M,Brenan L,Cohen O,Kitajima S,Nayar U,Walker A,Pantel S,Lee Y,Cordova J,Sathappa M,Zhu C,Hayes TK,Ram P,Pancholi P,Mikkelsen TS,Barbie DA,Yang X,Haq R,Piccioni F,Root DE,Johannes

    更新日期：2020-01-01 00:00:00

  • Efficiency and specificity in microRNA biogenesis.

    abstract：:Primary microRNA cleavage by the Drosha-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the au...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2293

    authors： Barad O,Mann M,Chapnik E,Shenoy A,Blelloch R,Barkai N,Hornstein E

    更新日期：2012-05-13 00:00:00

  • Structure of full-length human anti-PD1 therapeutic IgG4 antibody pembrolizumab.

    abstract：:Immunoglobulin G4 antibodies exhibit unusual properties with important biological consequences. We report the structure of the human full-length IgG4 S228P anti-PD1 antibody pembrolizumab, solved to 2.3-Å resolution. Pembrolizumab is a compact molecule, consistent with the presence of a short hinge region. The Fc doma...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3129

    authors： Scapin G,Yang X,Prosise WW,McCoy M,Reichert P,Johnston JM,Kashi RS,Strickland C

    更新日期：2015-12-01 00:00:00

  • Evolutionary conservation of codon optimality reveals hidden signatures of cotranslational folding.

    abstract：:The choice of codons can influence local translation kinetics during protein synthesis. Whether codon preference is linked to cotranslational regulation of polypeptide folding remains unclear. Here, we derive a revised translational efficiency scale that incorporates the competition between tRNA supply and demand. App...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2466

    authors： Pechmann S,Frydman J

    更新日期：2013-02-01 00:00:00

  • Author Correction: Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature structural & molecular biology

    pub_type： 已发布勘误

    doi：10.1038/s41594-020-0498-1

    authors： Mahalingan KK,Keith Keenan E,Strickland M,Li Y,Liu Y,Ball HL,Tanner ME,Tjandra N,Roll-Mecak A

    更新日期：2020-09-01 00:00:00

  • Location, location, location: a crystal-clear view of autotaxin saturating LPA receptors.

    abstract：:The interaction of autotaxin with its substrates leads to the production of lysophosphatidic acids (LPA), bioactive lipids with an emerging prominent role in inflammation and cancer. Two papers in this issue tell the previously unknown story of autotaxin, from substrate discrimination to highly efficient local deliver...

    journal_title：Nature structural & molecular biology

    pub_type： 评论,杂志文章

    doi：10.1038/nsmb0211-117

    authors： Tabchy A,Tigyi G,Mills GB

    更新日期：2011-02-01 00:00:00

  • DNA-RNA hybrids: the risks of DNA breakage during transcription.

    abstract：:Although R loops can occur at different genomic locations, the factors that determine their formation and frequency remain unclear. Emerging evidence indicates that DNA breaks stimulate DNA-RNA hybrid formation. Here, we discuss the possibility that formation of hybrids may be an inevitable risk of DNA breaks that occ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3395

    authors： Aguilera A,Gómez-González B

    更新日期：2017-05-04 00:00:00

  • Structure of the Dom34-Hbs1 complex and implications for no-go decay.

    abstract：:No-go decay (NGD) targets mRNAs with stalls in translation elongation for endonucleolytic cleavage in a process involving the Dom34 and Hbs1 proteins. The crystal structure of a Schizosaccharomyces pombe Dom34-Hbs1 complex reveals an overall shape similar to that of eRF1-eRF3-GTP and EF-Tu-tRNA-GDPNP. Similarly to eRF...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1922

    authors： Chen L,Muhlrad D,Hauryliuk V,Cheng Z,Lim MK,Shyp V,Parker R,Song H

    更新日期：2010-10-01 00:00:00

  • Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain.

    abstract：:The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of n...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1543

    authors： Quistgaard EM,Madsen P,Grøftehauge MK,Nissen P,Petersen CM,Thirup SS

    更新日期：2009-01-01 00:00:00

  • Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53.

    abstract：:In eukaryotes, the essential dimeric molecular chaperone Hsp90 is required for the activation and maturation of specific substrates such as steroid hormone receptors, tyrosine kinases and transcription factors. Hsp90 is involved in the establishment of cancer and has become an attractive target for drug design. Here w...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2114

    authors： Hagn F,Lagleder S,Retzlaff M,Rohrberg J,Demmer O,Richter K,Buchner J,Kessler H

    更新日期：2011-09-04 00:00:00

  • ATP-dependent chromatin remodeling shapes the DNA replication landscape.

    abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1419

    authors： Vincent JA,Kwong TJ,Tsukiyama T

    更新日期：2008-05-01 00:00:00

  • Proton-powered subunit rotation in single membrane-bound F0F1-ATP synthase.

    abstract：:Synthesis of ATP from ADP and phosphate, catalyzed by F(0)F(1)-ATP synthases, is the most abundant physiological reaction in almost any cell. F(0)F(1)-ATP synthases are membrane-bound enzymes that use the energy derived from an electrochemical proton gradient for ATP formation. We incorporated double-labeled F(0)F(1)-...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb718

    authors： Diez M,Zimmermann B,Börsch M,König M,Schweinberger E,Steigmiller S,Reuter R,Felekyan S,Kudryavtsev V,Seidel CA,Gräber P

    更新日期：2004-02-01 00:00:00

  • Fast ribozyme cleavage releases transcripts from RNA polymerase II and aborts co-transcriptional pre-mRNA processing.

    abstract：:We investigated whether a continuous transcript is necessary for co-transcriptional pre-mRNA processing. Cutting an intron with the fast-cleaving hepatitis delta ribozyme, but not the slower hammerhead, inhibited splicing. Therefore, exon tethering to RNA polymerase II (Pol II) cannot rescue splicing of a transcript s...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1652

    authors： Fong N,Ohman M,Bentley DL

    更新日期：2009-09-01 00:00:00

  • CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage.

    abstract：:In budding yeast, DNA damage in G1 activates a Rad9-dependent checkpoint that targets the cyclin-dependent kinase (CDK) Cdc28 to delay G1 exit. After a transient arrest, cells may enter S phase before completing DNA repair. We used genetic analysis to identify the stress-responsive CDK Pho85, the cyclin Pho80 and the ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1139

    authors： Wysocki R,Javaheri A,Kristjansdottir K,Sha F,Kron SJ

    更新日期：2006-10-01 00:00:00

  • BAK core dimers bind lipids and can be bridged by them.

    abstract：:BAK and BAX are essential mediators of apoptosis that oligomerize in response to death cues, thereby causing permeabilization of the mitochondrial outer membrane. Their transition from quiescent monomers to pore-forming oligomers involves a well-characterized symmetric dimer intermediate. However, no essential seconda...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0494-5

    authors： Cowan AD,Smith NA,Sandow JJ,Kapp EA,Rustam YH,Murphy JM,Brouwer JM,Bernardini JP,Roy MJ,Wardak AZ,Tan IK,Webb AI,Gulbis JM,Smith BJ,Reid GE,Dewson G,Colman PM,Czabotar PE

    更新日期：2020-11-01 00:00:00

  • Insertion of an Alu element in a lncRNA leads to primate-specific modulation of alternative splicing.

    abstract：:Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryot...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3302

    authors： Hu S,Wang X,Shan G

    更新日期：2016-11-01 00:00:00

  • Structure of the measles virus hemagglutinin bound to the CD46 receptor.

    abstract：:The highly contagious measles virus infects millions of individuals worldwide, causing serious disease in children of developing countries. Infection is initiated by attachment of the measles virus hemagglutinin (MV-H), a glycoprotein anchored to the virus envelope, to the host cell receptors CD46 or signaling lymphoc...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1726

    authors： Santiago C,Celma ML,Stehle T,Casasnovas JM

    更新日期：2010-01-01 00:00:00