当前位置： SCI文献检索 > NATURE STRUCTURAL & MOLECULAR BIOLOGY期刊下所有文献 > Structure of the Dom34-Hbs1 complex and implications for no-go decay.

Structure of the Dom34-Hbs1 complex and implications for no-go decay.

Abstract：

:No-go decay (NGD) targets mRNAs with stalls in translation elongation for endonucleolytic cleavage in a process involving the Dom34 and Hbs1 proteins. The crystal structure of a Schizosaccharomyces pombe Dom34-Hbs1 complex reveals an overall shape similar to that of eRF1-eRF3-GTP and EF-Tu-tRNA-GDPNP. Similarly to eRF1 and GTP binding to eRF3, Dom34 and GTP bind to Hbs1 with strong cooperativity, and Dom34 acts as a GTP-dissociation inhibitor (GDI). A marked conformational change in Dom34 occurs upon binding to Hbs1, leading Dom34 to resemble a portion of a tRNA and to position a conserved basic region in a position expected to be near the peptidyl transferase center. These results support the idea that the Dom34-Hbs1 complex functions to terminate translation and thereby commit mRNAs to NGD. Consistent with this role, NGD at runs of arginine codons, which cause a strong block to elongation, is independent of the Dom34-Hbs1 complex.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Chen L,Muhlrad D,Hauryliuk V,Cheng Z,Lim MK,Shyp V,Parker R,Song H

doi

10.1038/nsmb.1922

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

1233-40

issue

10

eissn

1545-9993

issn

1545-9985

pii

nsmb.1922

journal_volume

17

pub_type

杂志文章

相关文献

NATURE STRUCTURAL & MOLECULAR BIOLOGY文献大全
  • Let K-Ras activate its own inhibitor.

    abstract：: ...

    journal_title：Nature structural & molecular biology

    pub_type： 新闻

    doi：10.1038/s41594-018-0066-0

    authors： Statsyuk AV

    更新日期：2018-06-01 00:00:00

  • Reply to 'Misreading chaperone-substrate complexes from random noise'.

    abstract：: ...

    journal_title：Nature structural & molecular biology

    pub_type： 评论,信件

    doi：10.1038/s41594-018-0145-2

    authors： Horowitz S,Salmon L,Koldewey P,Ahlstrom LS,Martin R,Quan S,Afonine PV,van den Bedem H,Wang L,Xu Q,Trievel RC,Brooks CL 3rd,Bardwell JCA

    更新日期：2018-11-01 00:00:00

  • A two-pronged strategy to suppress host protein synthesis by SARS coronavirus Nsp1 protein.

    abstract：:Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression, including type I interferon production, by promoting host mRNA degradation and inhibiting host translation, in infected cells. We present evidence that nsp1 uses a novel, two-pronged strategy to inhibit host translation and gen...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1680

    authors： Kamitani W,Huang C,Narayanan K,Lokugamage KG,Makino S

    更新日期：2009-11-01 00:00:00

  • 7SK-BAF axis controls pervasive transcription at enhancers.

    abstract：:RNA functions at enhancers remain mysterious. Here we show that the 7SK small nuclear RNA (snRNA) inhibits enhancer transcription by modulating nucleosome position. 7SK occupies enhancers and super enhancers genome wide in mouse and human cells, and it is required to limit enhancer-RNA initiation and synthesis in a ma...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3176

    authors： Flynn RA,Do BT,Rubin AJ,Calo E,Lee B,Kuchelmeister H,Rale M,Chu C,Kool ET,Wysocka J,Khavari PA,Chang HY

    更新日期：2016-03-01 00:00:00

  • Polypyrimidine tract binding protein controls the transition from exon definition to an intron defined spliceosome.

    abstract：:The polypyrimidine tract binding protein (PTB) binds pre-mRNAs to alter splice-site choice. We characterized a series of spliceosomal complexes that assemble on a pre-mRNA under conditions of either PTB-mediated splicing repression or its absence. In the absence of repression, exon definition complexes that were assem...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1375

    authors： Sharma S,Kohlstaedt LA,Damianov A,Rio DC,Black DL

    更新日期：2008-02-01 00:00:00

  • A molecular mechanism for calcium-mediated synaptotagmin-triggered exocytosis.

    abstract：:The regulated exocytotic release of neurotransmitter and hormones is accomplished by a complex protein machinery whose core consists of SNARE proteins and the calcium sensor synaptotagmin-1. We propose a mechanism in which the lipid membrane is intimately involved in coupling calcium sensing to release. We found that ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0130-9

    authors： Kiessling V,Kreutzberger AJB,Liang B,Nyenhuis SB,Seelheim P,Castle JD,Cafiso DS,Tamm LK

    更新日期：2018-10-01 00:00:00

  • A newly discovered function for RNase L in regulating translation termination.

    abstract：:The antiviral and antiproliferative effects of interferons are mediated in part by the 2'-5' oligoadenylate-RNase L RNA decay pathway. RNase L is an endoribonuclease that requires 2'-5' oligoadenylates to cleave single-stranded RNA. In this report we present evidence demonstrating a role for RNase L in translation. We...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb944

    authors： Le Roy F,Salehzada T,Bisbal C,Dougherty JP,Peltz SW

    更新日期：2005-06-01 00:00:00

  • Structural basis of the proinflammatory signaling complex mediated by TSLP.

    abstract：:Thymic stromal lymphopoietin (TSLP), a cytokine produced by epithelial cells at barrier surfaces, is pivotal for the development of widespread chronic inflammatory disorders such as asthma and atopic dermatitis. The structure of the mouse TSLP-mediated signaling complex reveals how TSLP establishes extensive interface...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2794

    authors： Verstraete K,van Schie L,Vyncke L,Bloch Y,Tavernier J,Pauwels E,Peelman F,Savvides SN

    更新日期：2014-04-01 00:00:00

  • Structural basis for RNA recognition by a dimeric PPR-protein complex.

    abstract：:Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Br...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2710

    authors： Ke J,Chen RZ,Ban T,Zhou XE,Gu X,Tan MH,Chen C,Kang Y,Brunzelle JS,Zhu JK,Melcher K,Xu HE

    更新日期：2013-12-01 00:00:00

  • Structural basis for the coevolution of a viral RNA-protein complex.

    abstract：:The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to s...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1327

    authors： Chao JA,Patskovsky Y,Almo SC,Singer RH

    更新日期：2008-01-01 00:00:00

  • Structural basis for selective activation of ABA receptors.

    abstract：:Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discov...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1898

    authors： Peterson FC,Burgie ES,Park SY,Jensen DR,Weiner JJ,Bingman CA,Chang CE,Cutler SR,Phillips GN Jr,Volkman BF

    更新日期：2010-09-01 00:00:00

  • A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain.

    abstract：:Interactions between Src homology 2 (SH2) domains and phosphotyrosine sites regulate tyrosine kinase signaling networks. Selective perturbation of these interactions is challenging due to the high homology among the 120 human SH2 domains. Using an improved phage-display selection system, we generated a small antibody ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1793

    authors： Wojcik J,Hantschel O,Grebien F,Kaupe I,Bennett KL,Barkinge J,Jones RB,Koide A,Superti-Furga G,Koide S

    更新日期：2010-04-01 00:00:00

  • Structure and mechanism of the RNA polymerase II transcription machinery.

    abstract：:Advances in structure determination of the bacterial and eukaryotic transcription machinery have led to a marked increase in the understanding of the mechanism of transcription. Models for the specific assembly of the RNA polymerase II transcription machinery at a promoter, conformational changes that occur during ini...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb763

    authors： Hahn S

    更新日期：2004-05-01 00:00:00

  • Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.

    abstract：:The RNA-induced transcriptional silencing (RITS) complex, containing Ago1, Chp1, Tas3 and centromeric small interfering RNAs (siRNAs), is required for heterochromatic gene silencing at centromeres. Here, we identify a second fission yeast Argonaute complex (Argonaute siRNA chaperone, ARC), which contains, in addition ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1211

    authors： Buker SM,Iida T,Bühler M,Villén J,Gygi SP,Nakayama J,Moazed D

    更新日期：2007-03-01 00:00:00

  • ATP-dependent chromatin remodeling shapes the DNA replication landscape.

    abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1419

    authors： Vincent JA,Kwong TJ,Tsukiyama T

    更新日期：2008-05-01 00:00:00

  • Notch is a direct negative regulator of the DNA-damage response.

    abstract：:The DNA-damage response (DDR) ensures genome stability and proper inheritance of genetic information, both of which are essential to survival. It is presently unclear to what extent other signaling pathways modulate DDR function. Here we show that Notch receptor binds and inactivates ATM kinase and that this mechanism...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3013

    authors： Vermezovic J,Adamowicz M,Santarpia L,Rustighi A,Forcato M,Lucano C,Massimiliano L,Costanzo V,Bicciato S,Del Sal G,d'Adda di Fagagna F

    更新日期：2015-05-01 00:00:00

  • Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5.

    abstract：:RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the bind...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2596

    authors： Kassube SA,Jinek M,Fang J,Tsutakawa S,Nogales E

    更新日期：2013-07-01 00:00:00

  • STAT2 is an essential adaptor in USP18-mediated suppression of type I interferon signaling.

    abstract：:Type I interferons (IFNs) are multifunctional cytokines that regulate immune responses and cellular functions but also can have detrimental effects on human health. A tight regulatory network therefore controls IFN signaling, which in turn may interfere with medical interventions. The JAK-STAT signaling pathway transm...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3378

    authors： Arimoto KI,Löchte S,Stoner SA,Burkart C,Zhang Y,Miyauchi S,Wilmes S,Fan JB,Heinisch JJ,Li Z,Yan M,Pellegrini S,Colland F,Piehler J,Zhang DE

    更新日期：2017-03-01 00:00:00

  • Structural basis for -10 promoter element melting by environmentally induced sigma factors.

    abstract：:Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused re...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2777

    authors： Campagne S,Marsh ME,Capitani G,Vorholt JA,Allain FH

    更新日期：2014-03-01 00:00:00

  • Regulation of MLL1 H3K4 methyltransferase activity by its core components.

    abstract：:Histone H3 Lys4 (H3K4) methylation is a prevalent mark associated with transcription activation. A common feature of several H3K4 methyltransferase complexes is the presence of three structural components (RbBP5, Ash2L and WDR5) and a catalytic subunit containing a SET domain. Here we report the first biochemical reco...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1128

    authors： Dou Y,Milne TA,Ruthenburg AJ,Lee S,Lee JW,Verdine GL,Allis CD,Roeder RG

    更新日期：2006-08-01 00:00:00

  • An assessment of histone-modification antibody quality.

    abstract：:We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibo...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1972

    authors： Egelhofer TA,Minoda A,Klugman S,Lee K,Kolasinska-Zwierz P,Alekseyenko AA,Cheung MS,Day DS,Gadel S,Gorchakov AA,Gu T,Kharchenko PV,Kuan S,Latorre I,Linder-Basso D,Luu Y,Ngo Q,Perry M,Rechtsteiner A,Riddle NC,Schwar

    更新日期：2011-01-01 00:00:00

  • Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL.

    abstract：:Herpesviruses, which cause many incurable diseases, infect cells by fusing viral and cellular membranes. Whereas most other enveloped viruses use a single viral catalyst called a fusogen, herpesviruses, inexplicably, require two conserved fusion-machinery components, gB and the heterodimer gH-gL, plus other nonconserv...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1837

    authors： Chowdary TK,Cairns TM,Atanasiu D,Cohen GH,Eisenberg RJ,Heldwein EE

    更新日期：2010-07-01 00:00:00

  • Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.

    abstract：:Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatch...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1823

    authors： Liu X,Zhang Q,Murata K,Baker ML,Sullivan MB,Fu C,Dougherty MT,Schmid MF,Osburne MS,Chisholm SW,Chiu W

    更新日期：2010-07-01 00:00:00

  • CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage.

    abstract：:In budding yeast, DNA damage in G1 activates a Rad9-dependent checkpoint that targets the cyclin-dependent kinase (CDK) Cdc28 to delay G1 exit. After a transient arrest, cells may enter S phase before completing DNA repair. We used genetic analysis to identify the stress-responsive CDK Pho85, the cyclin Pho80 and the ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1139

    authors： Wysocki R,Javaheri A,Kristjansdottir K,Sha F,Kron SJ

    更新日期：2006-10-01 00:00:00

  • Tertiary interactions within the ribosomal exit tunnel.

    abstract：:Although tertiary folding of whole protein domains is prohibited by the cramped dimensions of the ribosomal tunnel, dynamic tertiary interactions may permit folding of small elementary units within the tunnel. To probe this possibility, we used a beta-hairpin and an alpha-helical hairpin from the cytosolic N terminus ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1571

    authors： Kosolapov A,Deutsch C

    更新日期：2009-04-01 00:00:00

  • Nemo kinase phosphorylates β-catenin to promote ommatidial rotation and connects core PCP factors to E-cadherin-β-catenin.

    abstract：:Frizzled planar cell polarity (PCP) signaling regulates cell motility in several tissues, including ommatidial rotation in Drosophila melanogaster. The Nemo kinase (Nlk in vertebrates) has also been linked to cell-motility regulation and ommatidial rotation but its mechanistic role(s) during rotation remain obscure. W...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2049

    authors： Mirkovic I,Gault WJ,Rahnama M,Jenny A,Gaengel K,Bessette D,Gottardi CJ,Verheyen EM,Mlodzik M

    更新日期：2011-06-01 00:00:00

  • Single-molecule analysis of protein-free U2-U6 snRNAs.

    abstract：:Spliceosomes catalyze the maturation of precursor mRNAs in organisms ranging from yeast to humans. Their catalytic core comprises three small nuclear RNAs (U2, U5 and U6) involved in substrate positioning and catalysis. It has been postulated, but never shown experimentally, that the U2-U6 complex adopts at least two ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1672

    authors： Guo Z,Karunatilaka KS,Rueda D

    更新日期：2009-11-01 00:00:00

  • PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs.

    abstract：:Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJC and the translation machinery has not been identified. The EJC prot...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1321

    authors： Diem MD,Chan CC,Younis I,Dreyfuss G

    更新日期：2007-12-01 00:00:00

  • Rps26 directs mRNA-specific translation by recognition of Kozak sequence elements.

    abstract：:We describe a novel approach to separate two ribosome populations from the same cells and use this method in combination with RNA-seq to identify mRNAs bound to Saccharomyces cerevisiae ribosomes with and without Rps26, a protein linked to the pathogenesis of Diamond-Blackfan anemia (DBA). These analyses reveal that R...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3442

    authors： Ferretti MB,Ghalei H,Ward EA,Potts EL,Karbstein K

    更新日期：2017-09-01 00:00:00

  • Incision-dependent and error-free repair of (CAG)(n)/(CTG)(n) hairpins in human cell extracts.

    abstract：:Expansion of CAG/CTG trinucleotide repeats is associated with certain familial neurological disorders, including Huntington's disease. Increasing evidence suggests that formation of a stable DNA hairpin within CAG/CTG repeats during DNA metabolism contributes to their expansion. However, the molecular mechanism(s) by ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1638

    authors： Hou C,Chan NL,Gu L,Li GM

    更新日期：2009-08-01 00:00:00