当前位置： SCI文献检索 > NATURE STRUCTURAL & MOLECULAR BIOLOGY期刊下所有文献 > Single-molecule analysis of protein-free U2-U6 snRNAs.

Single-molecule analysis of protein-free U2-U6 snRNAs.

Abstract：

:Spliceosomes catalyze the maturation of precursor mRNAs in organisms ranging from yeast to humans. Their catalytic core comprises three small nuclear RNAs (U2, U5 and U6) involved in substrate positioning and catalysis. It has been postulated, but never shown experimentally, that the U2-U6 complex adopts at least two conformations that reflect different activation states. We have used single-molecule fluorescence to probe the structural dynamics of a protein-free RNA complex modeling U2-U6 from yeast and mutants of highly conserved regions of U2-U6. Our data show the presence of at least three distinct conformations in equilibrium. The minimal folding pathway consists of a two-step process with an obligatory intermediate. The first step is strongly magnesium dependent, and we provide evidence suggesting that the second step corresponds to the formation of the genetically conserved helix IB. Site-specific mutations in the highly conserved AGC triad and the U80 base in U6 suggest that the observed conformational dynamics correlate with residues that have an important role in splicing.

journal_name

Nat Struct Mol Biol

journal_title

Nature structural & molecular biology

authors

Guo Z,Karunatilaka KS,Rueda D

doi

10.1038/nsmb.1672

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

1154-9

issue

11

eissn

1545-9993

issn

1545-9985

pii

nsmb.1672

journal_volume

16

pub_type

杂志文章

相关文献

NATURE STRUCTURAL & MOLECULAR BIOLOGY文献大全
  • Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53.

    abstract：:In eukaryotes, the essential dimeric molecular chaperone Hsp90 is required for the activation and maturation of specific substrates such as steroid hormone receptors, tyrosine kinases and transcription factors. Hsp90 is involved in the establishment of cancer and has become an attractive target for drug design. Here w...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2114

    authors： Hagn F,Lagleder S,Retzlaff M,Rohrberg J,Demmer O,Richter K,Buchner J,Kessler H

    更新日期：2011-09-04 00:00:00

  • PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs.

    abstract：:Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJC and the translation machinery has not been identified. The EJC prot...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1321

    authors： Diem MD,Chan CC,Younis I,Dreyfuss G

    更新日期：2007-12-01 00:00:00

  • Reply to 'Misreading chaperone-substrate complexes from random noise'.

    abstract：: ...

    journal_title：Nature structural & molecular biology

    pub_type： 评论,信件

    doi：10.1038/s41594-018-0145-2

    authors： Horowitz S,Salmon L,Koldewey P,Ahlstrom LS,Martin R,Quan S,Afonine PV,van den Bedem H,Wang L,Xu Q,Trievel RC,Brooks CL 3rd,Bardwell JCA

    更新日期：2018-11-01 00:00:00

  • T-cell receptor recognition of HLA-DQ2-gliadin complexes associated with celiac disease.

    abstract：:Celiac disease is a T cell-mediated disease induced by dietary gluten, a component of which is gliadin. 95% of individuals with celiac disease carry the HLA (human leukocyte antigen)-DQ2 locus. Here we determined the T-cell receptor (TCR) usage and fine specificity of patient-derived T-cell clones specific for two epi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2817

    authors： Petersen J,Montserrat V,Mujico JR,Loh KL,Beringer DX,van Lummel M,Thompson A,Mearin ML,Schweizer J,Kooy-Winkelaar Y,van Bergen J,Drijfhout JW,Kan WT,La Gruta NL,Anderson RP,Reid HH,Koning F,Rossjohn J

    更新日期：2014-05-01 00:00:00

  • Computational redesign of protein-protein interaction specificity.

    abstract：:We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb749

    authors： Kortemme T,Joachimiak LA,Bullock AN,Schuler AD,Stoddard BL,Baker D

    更新日期：2004-04-01 00:00:00

  • Structural basis for RNA recognition by a dimeric PPR-protein complex.

    abstract：:Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Br...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2710

    authors： Ke J,Chen RZ,Ban T,Zhou XE,Gu X,Tan MH,Chen C,Kang Y,Brunzelle JS,Zhu JK,Melcher K,Xu HE

    更新日期：2013-12-01 00:00:00

  • BRD4 assists elongation of both coding and enhancer RNAs by interacting with acetylated histones.

    abstract：:Small-molecule BET inhibitors interfere with the epigenetic interactions between acetylated histones and the bromodomains of the BET family proteins, including BRD4, and they potently inhibit growth of malignant cells by targeting cancer-promoting genes. BRD4 interacts with the pause-release factor P-TEFb and has been...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2912

    authors： Kanno T,Kanno Y,LeRoy G,Campos E,Sun HW,Brooks SR,Vahedi G,Heightman TD,Garcia BA,Reinberg D,Siebenlist U,O'Shea JJ,Ozato K

    更新日期：2014-12-01 00:00:00

  • CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells.

    abstract：:In mammalian cells, nonsense-mediated messenger RNA decay (NMD) targets newly synthesized nonsense-containing mRNA bound by the cap-binding-protein heterodimer CBP80-CBP20 and at least one exon-junction complex (EJC). An EJC includes the NMD factors Upf3 or Upf3X and Upf2, and Upf2 recruits Upf1. Once this pioneer tra...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb995

    authors： Hosoda N,Kim YK,Lejeune F,Maquat LE

    更新日期：2005-10-01 00:00:00

  • UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites.

    abstract：:Ubiquitination is a post-translational modification (PTM) that is essential for balancing numerous physiological processes. To enable delineation of protein ubiquitination at a site-specific level, we generated an antibody, denoted UbiSite, recognizing the C-terminal 13 amino acids of ubiquitin, which remain attached ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0084-y

    authors： Akimov V,Barrio-Hernandez I,Hansen SVF,Hallenborg P,Pedersen AK,Bekker-Jensen DB,Puglia M,Christensen SDK,Vanselow JT,Nielsen MM,Kratchmarova I,Kelstrup CD,Olsen JV,Blagoev B

    更新日期：2018-07-01 00:00:00

  • Cryo-EM structure of a human prion fibril with a hydrophobic, protease-resistant core.

    abstract：:Self-templating assemblies of the human prion protein are clinically associated with transmissible spongiform encephalopathies. Here we present the cryo-EM structure of a denaturant- and protease-resistant fibril formed in vitro spontaneously by a 9.7-kDa unglycosylated fragment of the human prion protein. This human ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0403-y

    authors： Glynn C,Sawaya MR,Ge P,Gallagher-Jones M,Short CW,Bowman R,Apostol M,Zhou ZH,Eisenberg DS,Rodriguez JA

    更新日期：2020-05-01 00:00:00

  • Defining the landscape of ATP-competitive inhibitor resistance residues in protein kinases.

    abstract：:Kinases are involved in disease development and modulation of their activity can be therapeutically beneficial. Drug-resistant mutant kinases are valuable tools in drug discovery efforts, but the prediction of mutants across the kinome is challenging. Here, we generate deep mutational scanning data to identify mutant ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-019-0358-z

    authors： Persky NS,Hernandez D,Do Carmo M,Brenan L,Cohen O,Kitajima S,Nayar U,Walker A,Pantel S,Lee Y,Cordova J,Sathappa M,Zhu C,Hayes TK,Ram P,Pancholi P,Mikkelsen TS,Barbie DA,Yang X,Haq R,Piccioni F,Root DE,Johannes

    更新日期：2020-01-01 00:00:00

  • Dynamically driven protein allostery.

    abstract：:Allosteric interactions are typically considered to proceed through a series of discrete changes in bonding interactions that alter the protein conformation. Here we show that allostery can be mediated exclusively by transmitted changes in protein motions. We have characterized the negatively cooperative binding of cA...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1132

    authors： Popovych N,Sun S,Ebright RH,Kalodimos CG

    更新日期：2006-09-01 00:00:00

  • Allosteric inhibition of antiapoptotic MCL-1.

    abstract：:MCL-1 is an antiapoptotic BCL-2 family protein that has emerged as a major pathogenic factor in human cancer. Like BCL-2, MCL-1 bears a surface groove whose function is to sequester the BH3 killer domains of proapoptotic BCL-2 family members, a mechanism harnessed by cancer cells to establish formidable apoptotic bloc...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3223

    authors： Lee S,Wales TE,Escudero S,Cohen DT,Luccarelli J,Gallagher CG,Cohen NA,Huhn AJ,Bird GH,Engen JR,Walensky LD

    更新日期：2016-06-01 00:00:00

  • Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation.

    abstract：:The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation. To distinguish between local displacement versus global unfolding mechanisms for complex disassem...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3015

    authors： Yang B,Stjepanovic G,Shen Q,Martin A,Hurley JH

    更新日期：2015-06-01 00:00:00

  • Two-way communications between ubiquitin-like modifiers and DNA.

    abstract：:Many aspects of nucleic acid metabolism, such as DNA replication, repair and transcription, are regulated by the post-translational modifiers ubiquitin and SUMO. Not surprisingly, DNA itself plays an integral part in determining the modification of most chromatin-associated targets. Conversely, ubiquitination or SUMOy...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb.2805

    authors： Ulrich HD

    更新日期：2014-04-01 00:00:00

  • Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature structural & molecular biology

    pub_type： 已发布勘误

    doi：10.1038/s41594-020-00527-9

    authors： Huo J,Le Bas A,Ruza RR,Duyvesteyn HME,Mikolajek H,Malinauskas T,Tan TK,Rijal P,Dumoux M,Ward PN,Ren J,Zhou D,Harrison PJ,Weckener M,Clare DK,Vogirala VK,Radecke J,Moynié L,Zhao Y,Gilbert-Jaramillo J,Knight ML,Tr

    更新日期：2020-11-01 00:00:00

  • Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.

    abstract：:The exon-junction complex (EJC) functionally links splicing to subsequent mRNA localization, translation and stability. Sequence-independent binding of the EJC core to RNA is ensured by the DEAD-box helicase eIF4AIII. Here, we identified the splicing factor CWC22 as a new eIF4AIII partner in flies and humans. CWC22 co...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2380

    authors： Barbosa I,Haque N,Fiorini F,Barrandon C,Tomasetto C,Blanchette M,Le Hir H

    更新日期：2012-10-01 00:00:00

  • RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1.

    abstract：:Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by degradation of nascent peptides. However, endogenous RQC-inducing sequences and the mechanism underlying the...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0393-9

    authors： Matsuo Y,Tesina P,Nakajima S,Mizuno M,Endo A,Buschauer R,Cheng J,Shounai O,Ikeuchi K,Saeki Y,Becker T,Beckmann R,Inada T

    更新日期：2020-04-01 00:00:00

  • Constitutive splicing and economies of scale in gene expression.

    abstract：:In eukaryotic cells, many introns are constitutively, rather than alternatively, spliced and therefore do not contribute to isoform diversification. It has remained unclear what functional roles such constitutive splicing provides. To explore this issue, we asked how splicing affects the efficiency with which individu...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-019-0226-x

    authors： Ding F,Elowitz MB

    更新日期：2019-06-01 00:00:00

  • Crystal structures of STING protein reveal basis for recognition of cyclic di-GMP.

    abstract：:STING functions as both an adaptor protein signaling cytoplasmic double-stranded DNA and a direct immunosensor of cyclic diguanylate monophosphate (c-di-GMP). The crystal structures of the C-terminal domain of human STING (STING(CTD)) and its complex with c-di-GMP reveal how STING recognizes c-di-GMP. In response to c...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2332

    authors： Shang G,Zhu D,Li N,Zhang J,Zhu C,Lu D,Liu C,Yu Q,Zhao Y,Xu S,Gu L

    更新日期：2012-06-24 00:00:00

  • Cation trafficking propels RNA hydrolysis.

    abstract：:Catalysis by members of the RNase H superfamily of enzymes is generally believed to require only two Mg2+ ions that are coordinated by active-site carboxylates. By examining the catalytic process of Bacillus halodurans RNase H1 in crystallo, however, we found that the two canonical Mg2+ ions and an additional K+ faile...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-018-0099-4

    authors： Samara NL,Yang W

    更新日期：2018-08-01 00:00:00

  • Molecular basis for H3K36me3 recognition by the Tudor domain of PHF1.

    abstract：:The PHD finger protein 1 (PHF1) is essential in epigenetic regulation and genome maintenance. Here we show that the Tudor domain of human PHF1 binds to histone H3 trimethylated at Lys36 (H3K36me3). We report a 1.9-Å resolution crystal structure of the Tudor domain in complex with H3K36me3 and describe the molecular me...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2435

    authors： Musselman CA,Avvakumov N,Watanabe R,Abraham CG,Lalonde ME,Hong Z,Allen C,Roy S,Nuñez JK,Nickoloff J,Kulesza CA,Yasui A,Côté J,Kutateladze TG

    更新日期：2012-12-01 00:00:00

  • Structure of the Vif-binding domain of the antiviral enzyme APOBEC3G.

    abstract：:The human APOBEC3G (A3G) DNA cytosine deaminase restricts and hypermutates DNA-based parasites including HIV-1. The viral infectivity factor (Vif) prevents restriction by triggering A3G degradation. Although the structure of the A3G catalytic domain is known, the structure of the N-terminal Vif-binding domain has prov...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.3033

    authors： Kouno T,Luengas EM,Shigematsu M,Shandilya SM,Zhang J,Chen L,Hara M,Schiffer CA,Harris RS,Matsuo H

    更新日期：2015-06-01 00:00:00

  • Assembling the mitochondrial outer membrane.

    abstract：:The general preprotein translocase of the outer mitochondrial membrane (TOM complex) transports virtually all mitochondrial precursor proteins, but cannot assemble outer-membrane precursors into functional complexes. A recently discovered sorting and assembly machinery (SAM complex) is essential for integration and as...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb852

    authors： Pfanner N,Wiedemann N,Meisinger C,Lithgow T

    更新日期：2004-11-01 00:00:00

  • Antisense transcripts are targets for activating small RNAs.

    abstract：:Agents that activate expression of specific genes to probe cellular pathways or alleviate disease would go beyond existing approaches for controlling gene expression. Duplex RNAs complementary to promoter regions can repress or activate gene expression. The mechanism of these promoter-directed antigene RNAs (agRNAs) h...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1444

    authors： Schwartz JC,Younger ST,Nguyen NB,Hardy DB,Monia BP,Corey DR,Janowski BA

    更新日期：2008-08-01 00:00:00

  • Methylation of H4 lysines 5, 8 and 12 by yeast Set5 calibrates chromatin stress responses.

    abstract：:Methylation of histones is central to chromatin regulation, and thus previously unknown mechanisms regulating genome function can be revealed through the discovery of new histone methyl marks. Here we identify Set5 as the first histone H4 methyltransferase, which monomethylates the critical H4 lysine residues 5, 8 and...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.2252

    authors： Green EM,Mas G,Young NL,Garcia BA,Gozani O

    更新日期：2012-02-19 00:00:00

  • Cabin1 restrains p53 activity on chromatin.

    abstract：:The tumor suppressor p53 has been proposed to bind target promoters upon genotoxic stress. However, recent evidence shows that p53 occupies some target promoters without such stress, suggesting that a negative regulator might render p53 transcriptionally inactive on these promoters. Here we show that calcineurin bindi...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb.1657

    authors： Jang H,Choi SY,Cho EJ,Youn HD

    更新日期：2009-09-01 00:00:00

  • Ribosome-associated protein quality control.

    abstract：:Protein synthesis by the ribosome can fail for numerous reasons including faulty mRNA, insufficient availability of charged tRNAs and genetic errors. All organisms have evolved mechanisms to recognize stalled ribosomes and initiate pathways for recycling, quality control and stress signaling. Here we review the discov...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章,评审

    doi：10.1038/nsmb.3147

    authors： Brandman O,Hegde RS

    更新日期：2016-01-01 00:00:00

  • Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.

    abstract：:The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its ...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/s41594-020-0388-6

    authors： Yamada T,Yoshida T,Kawamoto A,Mitsuoka K,Iwasaki K,Tsuge H

    更新日期：2020-03-01 00:00:00

  • Physical and functional coupling of RNA-dependent RNA polymerase and Dicer in the biogenesis of endogenous siRNAs.

    abstract：:Many classes of small RNA (sRNA) involved in RNA silencing are generated by double-stranded RNA (dsRNA) processing. Although principles of sRNA biogenesis have emerged, newly identified classes of sRNAs have features that suggest additional biogenesis mechanisms. Tetrahymena thermophila expresses one such class, compr...

    journal_title：Nature structural & molecular biology

    pub_type： 杂志文章

    doi：10.1038/nsmb1262

    authors： Lee SR,Collins K

    更新日期：2007-07-01 00:00:00