A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain.

abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

journal_title：Nature structural & molecular biology

authors： Vincent JA,Kwong TJ,Tsukiyama T

更新日期：2008-05-01 00:00:00

abstract：:We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-...

journal_title：Nature structural & molecular biology

authors： Taggart AJ,DeSimone AM,Shih JS,Filloux ME,Fairbrother WG

更新日期：2012-06-17 00:00:00

abstract：:p53 binds as a tetramer to DNA targets consisting of two decameric half-sites separated by a variable spacer. Here we present high-resolution crystal structures of complexes between p53 core-domain tetramers and DNA targets consisting of contiguous half-sites. In contrast to previously reported p53-DNA complexes that ...

journal_title：Nature structural & molecular biology

authors： Kitayner M,Rozenberg H,Rohs R,Suad O,Rabinovich D,Honig B,Shakked Z

更新日期：2010-04-01 00:00:00

abstract：:Maintenance of cell volume against osmotic change is crucial for proper cell functions. Leucine-rich repeat-containing 8 proteins are anion-selective channels that extrude anions to decrease the cell volume on cellular swelling. Here, we present the structure of human leucine-rich repeat-containing 8A, determined by s...

journal_title：Nature structural & molecular biology

authors： Kasuya G,Nakane T,Yokoyama T,Jia Y,Inoue M,Watanabe K,Nakamura R,Nishizawa T,Kusakizako T,Tsutsumi A,Yanagisawa H,Dohmae N,Hattori M,Ichijo H,Yan Z,Kikkawa M,Shirouzu M,Ishitani R,Nureki O

更新日期：2018-09-01 00:00:00

abstract：:Three recent studies converged on a specific protein-protein interface between TPP1 and telomerase as being crucial for the regulation of both telomerase recruitment and processivity in mammalian cells. An equivalent interaction appears to exist in budding yeast, making this a nearly universal means of telomerase regu...

journal_title：Nature structural & molecular biology

authors： Lue NF,Yu EY,Lei M

更新日期：2013-01-01 00:00:00

abstract：:The tandem zinc finger (TZF) domain of the protein TIS11d binds to the class II AU-rich element (ARE) in the 3' untranslated region (3' UTR) of target mRNAs and promotes their deadenylation and degradation. The NMR structure of the TIS11d TZF domain bound to the RNA sequence 5'-UUAUUUAUU-3' comprises a pair of novel C...

journal_title：Nature structural & molecular biology

authors： Hudson BP,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2004-03-01 00:00:00

abstract：:Ubiquitination is a post-translational modification (PTM) that is essential for balancing numerous physiological processes. To enable delineation of protein ubiquitination at a site-specific level, we generated an antibody, denoted UbiSite, recognizing the C-terminal 13 amino acids of ubiquitin, which remain attached ...

journal_title：Nature structural & molecular biology

authors： Akimov V,Barrio-Hernandez I,Hansen SVF,Hallenborg P,Pedersen AK,Bekker-Jensen DB,Puglia M,Christensen SDK,Vanselow JT,Nielsen MM,Kratchmarova I,Kelstrup CD,Olsen JV,Blagoev B

更新日期：2018-07-01 00:00:00

abstract：:Cholesterol-dependent cytolysins are bacterial protein toxins that bind to cholesterol-containing membranes, form oligomeric complexes and insert into the bilayer to create large aqueous pores. Membrane-dependent structural rearrangements required to initiate the oligomerization of perfringolysin O monomers have been ...

journal_title：Nature structural & molecular biology

authors： Ramachandran R,Tweten RK,Johnson AE

更新日期：2004-08-01 00:00:00

abstract：:We present a structural analysis of the folding transition states of three SH3 domains. Our results reveal that the secondary structure is not yet fully formed at this stage of folding and that the solvent is only partially excluded from the interior of the protein. Comparison of the members of the transition state en...

journal_title：Nature structural & molecular biology

authors： Lindorff-Larsen K,Vendruscolo M,Paci E,Dobson CM

更新日期：2004-05-01 00:00:00

abstract：:We investigated co-transcriptional recruitment of pre-mRNA processing factors to human genes. Capping factors associate with paused RNA polymerase II (pol II) at the 5' ends of quiescent genes. They also track throughout actively transcribed genes and accumulate with paused polymerase in the 3' flanking region. The 3'...

journal_title：Nature structural & molecular biology

authors： Glover-Cutter K,Kim S,Espinosa J,Bentley DL

更新日期：2008-01-01 00:00:00

abstract：:Although the two B-family human DNA polymerases, pol δ and pol ε, are responsible for the bulk of nuclear genome replication, at least 14 additional polymerases have roles in nuclear DNA repair and replication. In this issue, newly reported crystal structures of two specialized A-family polymerases, pol δ and pol ε, e...

journal_title：Nature structural & molecular biology

authors： Beard WA,Wilson SH

更新日期：2015-04-01 00:00:00

abstract：:Heterochromatin causes epigenetic repression that can be transmitted through multiple cell divisions. However, the mechanisms underlying silencing and stability of heterochromatin are not fully understood. We show that heterochromatin differs from euchromatin in histone turnover and identify histone deacetylase (HDAC)...

journal_title：Nature structural & molecular biology

authors： Aygün O,Mehta S,Grewal SI

更新日期：2013-05-01 00:00:00

abstract：:Gating the ion-permeation pathway in K(+) channels requires conformational changes in activation and inactivation gates. Here we have investigated the structural alterations associated with pH-dependent inactivation gating of the KcsA-Kv1.3 K(+) channel using solid-state NMR spectroscopy in direct reference to electro...

journal_title：Nature structural & molecular biology

authors： Ader C,Schneider R,Hornig S,Velisetty P,Wilson EM,Lange A,Giller K,Ohmert I,Martin-Eauclaire MF,Trauner D,Becker S,Pongs O,Baldus M

更新日期：2008-06-01 00:00:00

abstract：:MicroRNAs (miRNAs) interact with target sites located in the 3' untranslated regions (3' UTRs) of mRNAs to downregulate their expression when the appropriate miRNA is bound to target mRNA. To establish the functional importance of target-site localization in the 3' UTR, we modified the stop codon to extend the coding ...

journal_title：Nature structural & molecular biology

authors： Gu S,Jin L,Zhang F,Sarnow P,Kay MA

更新日期：2009-02-01 00:00:00

abstract：:The interaction of autotaxin with its substrates leads to the production of lysophosphatidic acids (LPA), bioactive lipids with an emerging prominent role in inflammation and cancer. Two papers in this issue tell the previously unknown story of autotaxin, from substrate discrimination to highly efficient local deliver...

journal_title：Nature structural & molecular biology

authors： Tabchy A,Tigyi G,Mills GB

更新日期：2011-02-01 00:00:00

abstract：:The WAVE regulatory complex (WRC) transmits information from the Rac GTPase to the actin nucleator Arp2/3 complex. We have reconstituted recombinant human and Drosophila WRC in several forms and shown that they are inactive toward Arp2/3 complex but can be activated by Rac in a nucleotide-dependent fashion. Our observ...

journal_title：Nature structural & molecular biology

authors： Ismail AM,Padrick SB,Chen B,Umetani J,Rosen MK

更新日期：2009-05-01 00:00:00

abstract：:Many genomic alterations associated with human diseases localize in noncoding regulatory elements located far from the promoters they regulate, making it challenging to link noncoding mutations or risk-associated variants with target genes. The range of action of a given set of enhancers is thought to be defined by in...

journal_title：Nature structural & molecular biology

authors： Martin D,Pantoja C,Fernández Miñán A,Valdes-Quezada C,Moltó E,Matesanz F,Bogdanović O,de la Calle-Mustienes E,Domínguez O,Taher L,Furlan-Magaril M,Alcina A,Cañón S,Fedetz M,Blasco MA,Pereira PS,Ovcharenko I,Recillas-Tar

更新日期：2011-06-01 00:00:00

abstract：:Expansion of CAG/CTG trinucleotide repeats is associated with certain familial neurological disorders, including Huntington's disease. Increasing evidence suggests that formation of a stable DNA hairpin within CAG/CTG repeats during DNA metabolism contributes to their expansion. However, the molecular mechanism(s) by ...

journal_title：Nature structural & molecular biology

authors： Hou C,Chan NL,Gu L,Li GM

更新日期：2009-08-01 00:00:00

abstract：:RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the bind...

journal_title：Nature structural & molecular biology

authors： Kassube SA,Jinek M,Fang J,Tsutakawa S,Nogales E

更新日期：2013-07-01 00:00:00

abstract：:The CDK8 kinase module (CKM) is a conserved, dissociable Mediator subcomplex whose component subunits were genetically linked to the RNA polymerase II (RNAPII) C-terminal domain (CTD) and individually recognized as transcriptional repressors before Mediator was identified as a pre-eminent complex in eukaryotic transcr...

journal_title：Nature structural & molecular biology

authors： Tsai KL,Sato S,Tomomori-Sato C,Conaway RC,Conaway JW,Asturias FJ

更新日期：2013-05-01 00:00:00

abstract：:P element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a guanosine triphosphate cofactor and the generation of long staggered DNA breaks during transposition. To gain insights into t...

journal_title：Nature structural & molecular biology

authors： Ghanim GE,Kellogg EH,Nogales E,Rio DC

更新日期：2019-11-01 00:00:00

abstract：:Polycomb repressive complex 2 (PRC2) trimethylates histone H3 at lysine 27 to mark genes for repression. We measured the dynamics of PRC2 binding on recombinant chromatin and free DNA at the single-molecule level using total internal reflection fluorescence (TIRF) microscopy. PRC2 preferentially binds free DNA with mu...

journal_title：Nature structural & molecular biology

authors： Choi J,Bachmann AL,Tauscher K,Benda C,Fierz B,Müller J

更新日期：2017-12-01 00:00:00

abstract：:During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation comp...

journal_title：Nature structural & molecular biology

authors： Bernecky C,Plitzko JM,Cramer P

更新日期：2017-10-01 00:00:00

abstract：:Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryot...

journal_title：Nature structural & molecular biology

authors： Losey HC,Ruthenburg AJ,Verdine GL

更新日期：2006-02-01 00:00:00

abstract：:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that a...

journal_title：Nature structural & molecular biology

authors： Takeshita D,Tomita K

更新日期：2012-01-15 00:00:00

abstract：:Type I interferons (IFNs) are multifunctional cytokines that regulate immune responses and cellular functions but also can have detrimental effects on human health. A tight regulatory network therefore controls IFN signaling, which in turn may interfere with medical interventions. The JAK-STAT signaling pathway transm...

journal_title：Nature structural & molecular biology

authors： Arimoto KI,Löchte S,Stoner SA,Burkart C,Zhang Y,Miyauchi S,Wilmes S,Fan JB,Heinisch JJ,Li Z,Yan M,Pellegrini S,Colland F,Piehler J,Zhang DE

更新日期：2017-03-01 00:00:00

abstract：:In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the ...

journal_title：Nature structural & molecular biology

authors： Yoshizawa S,Rasubala L,Ose T,Kohda D,Fourmy D,Maenaka K

更新日期：2005-02-01 00:00:00

abstract：:Changing environmental conditions and lessening fresh water supplies have sparked intense interest in understanding and manipulating abscisic acid (ABA) signaling, which controls adaptive responses to drought and other abiotic stressors. We recently discovered a selective ABA agonist, pyrabactin, and used it to discov...

journal_title：Nature structural & molecular biology

authors： Peterson FC,Burgie ES,Park SY,Jensen DR,Weiner JJ,Bingman CA,Chang CE,Cutler SR,Phillips GN Jr,Volkman BF

更新日期：2010-09-01 00:00:00

abstract：:The typical function of kinesins is to transport cargo along microtubules. Binding of ATP to microtubule-attached motile kinesins leads to cargo displacement. To better understand the nature of the conformational changes that lead to the power stroke that moves a kinesin's load along a microtubule, we determined the X...

journal_title：Nature structural & molecular biology

authors： Gigant B,Wang W,Dreier B,Jiang Q,Pecqueur L,Plückthun A,Wang C,Knossow M

更新日期：2013-08-01 00:00:00

abstract：:RNA functions at enhancers remain mysterious. Here we show that the 7SK small nuclear RNA (snRNA) inhibits enhancer transcription by modulating nucleosome position. 7SK occupies enhancers and super enhancers genome wide in mouse and human cells, and it is required to limit enhancer-RNA initiation and synthesis in a ma...

journal_title：Nature structural & molecular biology

authors： Flynn RA,Do BT,Rubin AJ,Calo E,Lee B,Kuchelmeister H,Rale M,Chu C,Kool ET,Wysocka J,Khavari PA,Chang HY

更新日期：2016-03-01 00:00:00