A popular engagement at the ends.

abstract：:Human telomeres are associated with ATM and the protein complex consisting of MRE11, RAD50 and NBS1 (MRN), which are central to maintaining genomic stability. Here we show that when targeted to telomeres, wild-type RAD50 downregulates telomeric association of TRF1, a negative regulator of telomere maintenance. TRF1 bi...

journal_title：Nature structural & molecular biology

authors： Wu Y,Xiao S,Zhu XD

更新日期：2007-09-01 00:00:00

abstract：:The Ca(2+) sensor synaptotagmin-1 is thought to trigger membrane fusion by binding to acidic membrane lipids and SNARE proteins. Previous work has shown that binding is mediated by electrostatic interactions that are sensitive to the ionic environment. However, the influence of divalent or polyvalent ions, at physiolo...

journal_title：Nature structural & molecular biology

authors： Park Y,Seo JB,Fraind A,Pérez-Lara A,Yavuz H,Han K,Jung SR,Kattan I,Walla PJ,Choi M,Cafiso DS,Koh DS,Jahn R

更新日期：2015-10-01 00:00:00

abstract：:In eukaryotes, the essential dimeric molecular chaperone Hsp90 is required for the activation and maturation of specific substrates such as steroid hormone receptors, tyrosine kinases and transcription factors. Hsp90 is involved in the establishment of cancer and has become an attractive target for drug design. Here w...

journal_title：Nature structural & molecular biology

authors： Hagn F,Lagleder S,Retzlaff M,Rohrberg J,Demmer O,Richter K,Buchner J,Kessler H

更新日期：2011-09-04 00:00:00

abstract：:The choice of codons can influence local translation kinetics during protein synthesis. Whether codon preference is linked to cotranslational regulation of polypeptide folding remains unclear. Here, we derive a revised translational efficiency scale that incorporates the competition between tRNA supply and demand. App...

journal_title：Nature structural & molecular biology

authors： Pechmann S,Frydman J

更新日期：2013-02-01 00:00:00

abstract：:Neuronal communication is mediated by Ca(2+)-triggered fusion of transmitter-filled synaptic vesicles with the presynaptic plasma membrane. Synaptotagmin I functions as a Ca(2+) sensor that regulates exocytosis, whereas soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins in th...

journal_title：Nature structural & molecular biology

authors： Chicka MC,Hui E,Liu H,Chapman ER

更新日期：2008-08-01 00:00:00

abstract：:In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA. Using purified components in the presence of ATP-γS, we have captured in vitro an intermediate in pre-RC assembly that contain...

journal_title：Nature structural & molecular biology

authors： Sun J,Evrin C,Samel SA,Fernández-Cid A,Riera A,Kawakami H,Stillman B,Speck C,Li H

更新日期：2013-08-01 00:00:00

abstract：: ...

journal_title：Nature structural & molecular biology

authors： Statsyuk AV

更新日期：2018-06-01 00:00:00

abstract：:Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal struc...

journal_title：Nature structural & molecular biology

authors： Huang J,Brown AF,Wu J,Xue J,Bley CJ,Rand DP,Wu L,Zhang R,Chen JJ,Lei M

更新日期：2014-06-01 00:00:00

abstract：:Small-molecule BET inhibitors interfere with the epigenetic interactions between acetylated histones and the bromodomains of the BET family proteins, including BRD4, and they potently inhibit growth of malignant cells by targeting cancer-promoting genes. BRD4 interacts with the pause-release factor P-TEFb and has been...

journal_title：Nature structural & molecular biology

authors： Kanno T,Kanno Y,LeRoy G,Campos E,Sun HW,Brooks SR,Vahedi G,Heightman TD,Garcia BA,Reinberg D,Siebenlist U,O'Shea JJ,Ozato K

更新日期：2014-12-01 00:00:00

abstract：:Platelet-activating-factor receptor (PAFR) responds to platelet-activating factor (PAF), a phospholipid mediator of cell-to-cell communication that exhibits diverse physiological effects. PAFR is considered an important drug target for treating asthma, inflammation and cardiovascular diseases. Here we report crystal s...

journal_title：Nature structural & molecular biology

authors： Cao C,Tan Q,Xu C,He L,Yang L,Zhou Y,Zhou Y,Qiao A,Lu M,Yi C,Han GW,Wang X,Li X,Yang H,Rao Z,Jiang H,Zhao Y,Liu J,Stevens RC,Zhao Q,Zhang XC,Wu B

更新日期：2018-06-01 00:00:00

abstract：:The N-end rule pathway is a regulated proteolytic system that targets proteins containing destabilizing N-terminal residues (N-degrons) for ubiquitination and proteasomal degradation in eukaryotes. The N-degrons of type 1 substrates contain an N-terminal basic residue that is recognized by the UBR box domain of the E3...

journal_title：Nature structural & molecular biology

authors： Choi WS,Jeong BC,Joo YJ,Lee MR,Kim J,Eck MJ,Song HK

更新日期：2010-10-01 00:00:00

abstract：:Mg-ADP release is considered to be a crucial process for the regulation and motility of kinesin. To gain insight into the structural basis of this process, we solved the atomic structures of kinesin superfamily protein-1A (KIF1A) during and after Mg(2+) release. On the basis of new structural and mutagenesis data, we ...

journal_title：Nature structural & molecular biology

authors： Nitta R,Okada Y,Hirokawa N

更新日期：2008-10-01 00:00:00

abstract：:The eukaryotic DNA replication machinery must traverse every nucleosome in the genome during S phase. As nucleosomes are generally inhibitory to DNA-dependent processes, chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved...

journal_title：Nature structural & molecular biology

authors： Vincent JA,Kwong TJ,Tsukiyama T

更新日期：2008-05-01 00:00:00

abstract：:Mutually exclusive splicing is a regulated means to generate protein diversity, but the underlying mechanisms are poorly understood. Here comparative genome analysis revealed the built-in intronic elements for controlling mutually exclusive splicing of the 14-3-3ξ pre-mRNA. These elements are clade specific but are ev...

journal_title：Nature structural & molecular biology

authors： Yang Y,Zhan L,Zhang W,Sun F,Wang W,Tian N,Bi J,Wang H,Shi D,Jiang Y,Zhang Y,Jin Y

更新日期：2011-02-01 00:00:00

abstract：:Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by c...

journal_title：Nature structural & molecular biology

authors： Schaub JR,Lu X,Doneske B,Shin YK,McNew JA

更新日期：2006-08-01 00:00:00

abstract：:The proteasome is the main ATP-dependent protease in eukaryotic cells and controls the concentration of many regulatory proteins in the cytosol and nucleus. Proteins are targeted to the proteasome by the covalent attachment of polyubiquitin chains. The ubiquitin modification serves as the proteasome recognition elemen...

journal_title：Nature structural & molecular biology

authors： Prakash S,Tian L,Ratliff KS,Lehotzky RE,Matouschek A

更新日期：2004-09-01 00:00:00

abstract：:Multiple Argonaute proteins are implicated in gene silencing by RNA interference (RNAi), but only one is known to be an endonuclease that can cleave target mRNAs. Chimeric Argonaute proteins now reveal an unexpected mechanism by which mutations distal to the catalytic center can unmask intrinsic catalytic activity, re...

journal_title：Nature structural & molecular biology

authors： Kidwell MA,Doudna JA

更新日期：2013-07-01 00:00:00

abstract：:During homologous recombination, Rad51 forms a nucleoprotein filament with single-stranded DNA (ssDNA) that undergoes strand exchange with homologous double-stranded DNA (dsDNA). Here, we use real-time analysis to show that strand exchange by fission yeast Rad51 proceeds via two distinct three-strand intermediates, C1...

journal_title：Nature structural & molecular biology

authors： Ito K,Murayama Y,Takahashi M,Iwasaki H

更新日期：2018-01-01 00:00:00

abstract：:Tripeptidyl peptidase II (TPP II) is the largest known eukaryotic protease (6 MDa). It is believed to act downstream of the 26S proteasome, cleaving tripeptides from the N termini of longer peptides, and it is implicated in numerous cellular processes. Here we report the structure of Drosophila TPP II determined by a ...

journal_title：Nature structural & molecular biology

authors： Chuang CK,Rockel B,Seyit G,Walian PJ,Schönegge AM,Peters J,Zwart PH,Baumeister W,Jap BK

更新日期：2010-08-01 00:00:00

abstract：:Microtubules are regulated by post-translational modifications of tubulin. The ligation and cleavage of the carboxy-terminal tyrosine of α-tubulin impact microtubule functions during mitosis, cardiomyocyte contraction and neuronal processes. Tubulin tyrosination and detyrosination are mediated by tubulin tyrosine liga...

journal_title：Nature structural & molecular biology

authors： Li F,Hu Y,Qi S,Luo X,Yu H

更新日期：2019-07-01 00:00:00

abstract：:Core Factor (CF) is a conserved RNA polymerase (Pol) I general transcription factor comprising Rrn6, Rrn11 and the TFIIB-related subunit Rrn7. CF binds TATA-binding protein (TBP), Pol I and the regulatory factors Rrn3 and upstream activation factor. We used chemical cross-linking-MS to determine the molecular architec...

journal_title：Nature structural & molecular biology

authors： Knutson BA,Luo J,Ranish J,Hahn S

更新日期：2014-09-01 00:00:00

abstract：:Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can ...

journal_title：Nature structural & molecular biology

authors： Sarlós K,Biebricher AS,Bizard AH,Bakx JAM,Ferreté-Bonastre AG,Modesti M,Paramasivam M,Yao Q,Peterman EJG,Wuite GJL,Hickson ID

更新日期：2018-09-01 00:00:00

abstract：:Expansion of CAG/CTG trinucleotide repeats is associated with certain familial neurological disorders, including Huntington's disease. Increasing evidence suggests that formation of a stable DNA hairpin within CAG/CTG repeats during DNA metabolism contributes to their expansion. However, the molecular mechanism(s) by ...

journal_title：Nature structural & molecular biology

authors： Hou C,Chan NL,Gu L,Li GM

更新日期：2009-08-01 00:00:00

abstract：:The typical function of kinesins is to transport cargo along microtubules. Binding of ATP to microtubule-attached motile kinesins leads to cargo displacement. To better understand the nature of the conformational changes that lead to the power stroke that moves a kinesin's load along a microtubule, we determined the X...

journal_title：Nature structural & molecular biology

authors： Gigant B,Wang W,Dreier B,Jiang Q,Pecqueur L,Plückthun A,Wang C,Knossow M

更新日期：2013-08-01 00:00:00

abstract：:Aggregation of proteins containing polyglutamine (polyQ) expansions characterizes many neurodegenerative disorders, including Huntington's disease. Molecular chaperones modulate the aggregation and toxicity of the huntingtin (Htt) protein by an ill-defined mechanism. Here we determine how the chaperonin TRiC suppresse...

journal_title：Nature structural & molecular biology

authors： Tam S,Spiess C,Auyeung W,Joachimiak L,Chen B,Poirier MA,Frydman J

更新日期：2009-12-01 00:00:00

abstract：:Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJC and the translation machinery has not been identified. The EJC prot...

journal_title：Nature structural & molecular biology

authors： Diem MD,Chan CC,Younis I,Dreyfuss G

更新日期：2007-12-01 00:00:00

abstract：:Myotonic dystrophy is an RNA gain-of-function disease caused by expanded CUG or CCUG repeats, which sequester the RNA binding protein MBNL1. Here we describe a newly discovered function for MBNL1 as a regulator of pre-miR-1 biogenesis and find that miR-1 processing is altered in heart samples from people with myotonic...

journal_title：Nature structural & molecular biology

authors： Rau F,Freyermuth F,Fugier C,Villemin JP,Fischer MC,Jost B,Dembele D,Gourdon G,Nicole A,Duboc D,Wahbi K,Day JW,Fujimura H,Takahashi MP,Auboeuf D,Dreumont N,Furling D,Charlet-Berguerand N

更新日期：2011-06-19 00:00:00

abstract：:Many aspects of nucleic acid metabolism, such as DNA replication, repair and transcription, are regulated by the post-translational modifiers ubiquitin and SUMO. Not surprisingly, DNA itself plays an integral part in determining the modification of most chromatin-associated targets. Conversely, ubiquitination or SUMOy...

journal_title：Nature structural & molecular biology

authors： Ulrich HD

更新日期：2014-04-01 00:00:00

abstract：:Lipoteichoic acids (LTAs) are essential cell-wall components in Gram-positive bacteria, including the human pathogen Staphylococcus aureus, contributing to cell adhesion, cell division and antibiotic resistance. Genetic evidence has suggested that LtaA is the flippase that mediates the translocation of the lipid-linke...

journal_title：Nature structural & molecular biology

authors： Zhang B,Liu X,Lambert E,Mas G,Hiller S,Veening JW,Perez C

更新日期：2020-06-01 00:00:00

abstract：:TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by...

journal_title：Nature structural & molecular biology

authors： Price AJ,Marzetta F,Lammers M,Ylinen LM,Schaller T,Wilson SJ,Towers GJ,James LC

更新日期：2009-10-01 00:00:00