The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.

abstract：:Glucokinase (GK) is a glucose-phosphorylating enzyme that regulates insulin release and hepatic metabolism, and its loss of function is implicated in diabetes pathogenesis. GK activators (GKAs) are attractive therapeutics in diabetes; however, clinical data indicate that their benefits can be offset by hypoglycemia, o...

journal_title：Nature structural & molecular biology

authors： Szlyk B,Braun CR,Ljubicic S,Patton E,Bird GH,Osundiji MA,Matschinsky FM,Walensky LD,Danial NN

更新日期：2014-01-01 00:00:00

abstract：:The endosomal sorting complex required for transport (ESCRT) machinery, including ESCRT-III, localizes to the midbody and participates in the membrane-abscission step of cytokinesis. The ESCRT-III protein charged multivesicular body protein 1B (CHMP1B) is required for recruitment of the MIT domain-containing protein s...

journal_title：Nature structural & molecular biology

authors： Yang D,Rismanchi N,Renvoisé B,Lippincott-Schwartz J,Blackstone C,Hurley JH

更新日期：2008-12-01 00:00:00

abstract：:The histone variant macroH2A1 regulates gene expression important for differentiation, stem-cell reprogramming and tumor suppression. Here, we demonstrate that in primary human cells, macroH2A1 participates in two physically and functionally distinct types of chromatin marked by either H3K27me3 or nine histone acetyla...

journal_title：Nature structural & molecular biology

authors： Chen H,Ruiz PD,Novikov L,Casill AD,Park JW,Gamble MJ

更新日期：2014-11-01 00:00:00

abstract：:Agents that activate expression of specific genes to probe cellular pathways or alleviate disease would go beyond existing approaches for controlling gene expression. Duplex RNAs complementary to promoter regions can repress or activate gene expression. The mechanism of these promoter-directed antigene RNAs (agRNAs) h...

journal_title：Nature structural & molecular biology

authors： Schwartz JC,Younger ST,Nguyen NB,Hardy DB,Monia BP,Corey DR,Janowski BA

更新日期：2008-08-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) play critical roles during tumorigenesis by functioning as scaffolds that regulate protein-protein, protein-DNA or protein-RNA interactions. Using a clinically guided genetic screening approach, we identified lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1), which is overexpre...

journal_title：Nature structural & molecular biology

authors： Zhang Y,He Q,Hu Z,Feng Y,Fan L,Tang Z,Yuan J,Shan W,Li C,Hu X,Tanyi JL,Fan Y,Huang Q,Montone K,Dang CV,Zhang L

更新日期：2016-06-01 00:00:00

abstract：:SIX1 interacts with EYA to form a bipartite transcription factor essential for mammalian development. Loss of function of this complex causes branchio-oto-renal (BOR) syndrome, whereas re-expression of SIX1 or EYA promotes metastasis. Here we describe the 2.0-Å structure of SIX1 bound to EYA2, which suggests a new DNA...

journal_title：Nature structural & molecular biology

authors： Patrick AN,Cabrera JH,Smith AL,Chen XS,Ford HL,Zhao R

更新日期：2013-04-01 00:00:00

abstract：:Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its gamma subunit (eEF1Bgamma) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by approximately 20% and selectively increases 80S ribosom...

journal_title：Nature structural & molecular biology

authors： Kim S,Kellner J,Lee CH,Coulombe PA

更新日期：2007-10-01 00:00:00

abstract：:We developed an experimental system for studying genome instability caused by fragile X (CGG)n repeats in mammalian cells. Our method uses a selectable cassette carrying the HyTK gene under the control of the FMR1 promoter with (CGG)n repeats in its 5' UTR, which is integrated into the unique RL5 site in murine erythr...

journal_title：Nature structural & molecular biology

authors： Kononenko AV,Ebersole T,Vasquez KM,Mirkin SM

更新日期：2018-08-01 00:00:00

abstract：:The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its ...

journal_title：Nature structural & molecular biology

authors： Yamada T,Yoshida T,Kawamoto A,Mitsuoka K,Iwasaki K,Tsuge H

更新日期：2020-03-01 00:00:00

abstract：:Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryot...

journal_title：Nature structural & molecular biology

authors： Losey HC,Ruthenburg AJ,Verdine GL

更新日期：2006-02-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression, including type I interferon production, by promoting host mRNA degradation and inhibiting host translation, in infected cells. We present evidence that nsp1 uses a novel, two-pronged strategy to inhibit host translation and gen...

journal_title：Nature structural & molecular biology

authors： Kamitani W,Huang C,Narayanan K,Lokugamage KG,Makino S

更新日期：2009-11-01 00:00:00

abstract：:Cells respond to changes in environmental conditions via orchestrated modifications in gene expression. For example, in response to heat shock, cells execute a program of gene-specific transcriptional activation and repression. Although the activation of genes upon heat shock has been widely studied, the mechanism of ...

journal_title：Nature structural & molecular biology

authors： Allen TA,Von Kaenel S,Goodrich JA,Kugel JF

更新日期：2004-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature structural & molecular biology

authors： Koch A,Aguilera L,Morisaki T,Munsky B,Stasevich TJ

更新日期：2020-12-01 00:00:00

abstract：:The Mre11-Rad50-NBS1 (MRN) complex has many roles in response to DNA double-strand breaks, but its functions in repair by nonhomologous end joining (NHEJ) pathways are poorly understood. We have investigated requirements for MRN in class switch recombination (CSR), a programmed DNA rearrangement in B lymphocytes that ...

journal_title：Nature structural & molecular biology

authors： Dinkelmann M,Spehalski E,Stoneham T,Buis J,Wu Y,Sekiguchi JM,Ferguson DO

更新日期：2009-08-01 00:00:00

abstract：: ...

journal_title：Nature structural & molecular biology

authors： Statsyuk AV

更新日期：2018-06-01 00:00:00

abstract：:Bacteriophage T4 and related viruses have a contractile tail that serves as an efficient mechanical device for infecting bacteria. A three-dimensional cryo-EM reconstruction of the mature T4 tail assembly at 15-A resolution shows the hexagonal dome-shaped baseplate, the extended contractile sheath, the long tail fiber...

journal_title：Nature structural & molecular biology

authors： Kostyuchenko VA,Chipman PR,Leiman PG,Arisaka F,Mesyanzhinov VV,Rossmann MG

更新日期：2005-09-01 00:00:00

abstract：:The human APOBEC3G (A3G) DNA cytosine deaminase restricts and hypermutates DNA-based parasites including HIV-1. The viral infectivity factor (Vif) prevents restriction by triggering A3G degradation. Although the structure of the A3G catalytic domain is known, the structure of the N-terminal Vif-binding domain has prov...

journal_title：Nature structural & molecular biology

authors： Kouno T,Luengas EM,Shigematsu M,Shandilya SM,Zhang J,Chen L,Hara M,Schiffer CA,Harris RS,Matsuo H

更新日期：2015-06-01 00:00:00

abstract：:To our knowledge, no structural study to date has characterized, in an intact receptor, the coupling of conformational change in extracellular domains through a single-pass transmembrane domain to conformational change in cytoplasmic domains. Here we examine such coupling, and its unexpected complexity, using nearly f...

journal_title：Nature structural & molecular biology

authors： Mi LZ,Lu C,Li Z,Nishida N,Walz T,Springer TA

更新日期：2011-08-07 00:00:00

abstract：:Processing of microRNAs (miRNAs) from their precursors to their biologically active mature forms is regulated during development and cancer. We show that mouse pri- or pre-miR-151 can bind to and compete with mature miR-151-5p and miR-151-3p for binding sites contained within the complementary regions of the E2f6 mRNA...

journal_title：Nature structural & molecular biology

authors： Roy-Chaudhuri B,Valdmanis PN,Zhang Y,Wang Q,Luo QJ,Kay MA

更新日期：2014-09-01 00:00:00

abstract：:In eukaryotic cells, many introns are constitutively, rather than alternatively, spliced and therefore do not contribute to isoform diversification. It has remained unclear what functional roles such constitutive splicing provides. To explore this issue, we asked how splicing affects the efficiency with which individu...

journal_title：Nature structural & molecular biology

authors： Ding F,Elowitz MB

更新日期：2019-06-01 00:00:00

abstract：:Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall qua...

journal_title：Nature structural & molecular biology

authors： Gautier A,Mott HR,Bostock MJ,Kirkpatrick JP,Nietlispach D

更新日期：2010-06-01 00:00:00

abstract：:HIV-1 capsid plays multiple key roles in viral replication, and inhibition of capsid assembly is an attractive target for therapeutic intervention. Here, we report the atomic-resolution structure of capsid protein (CA) tubes, determined by magic-angle spinning NMR and data-guided molecular dynamics simulations. Functi...

journal_title：Nature structural & molecular biology

authors： Lu M,Russell RW,Bryer AJ,Quinn CM,Hou G,Zhang H,Schwieters CD,Perilla JR,Gronenborn AM,Polenova T

更新日期：2020-09-01 00:00:00

abstract：:Translation has a fundamental function in defining the fate of the transcribed genome. RNA-sequencing (RNA-seq) data enable the quantification of complex transcript mixtures, often detecting several transcript isoforms of unknown functions for one gene. Here, we describe ORFquant, a method to annotate and quantify tra...

journal_title：Nature structural & molecular biology

authors： Calviello L,Hirsekorn A,Ohler U

更新日期：2020-08-01 00:00:00

abstract：:The function of human XPA protein, a key subunit of the nucleotide excision repair pathway, has been examined with site-directed substitutions in its putative DNA-binding cleft. After screening for repair activity in a host-cell reactivation assay, we analyzed mutants by comparing their affinities for different substr...

journal_title：Nature structural & molecular biology

authors： Camenisch U,Dip R,Schumacher SB,Schuler B,Naegeli H

更新日期：2006-03-01 00:00:00

abstract：:Although DNA replication is a fundamental aspect of biology, it is not known what determines where DNA replication starts and stops in the human genome. We directly identified and quantitatively compared sites of replication initiation and termination in untransformed human cells. We found that replication preferentia...

journal_title：Nature structural & molecular biology

authors： Chen YH,Keegan S,Kahli M,Tonzi P,Fenyö D,Huang TT,Smith DJ

更新日期：2019-01-01 00:00:00

abstract：:Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Br...

journal_title：Nature structural & molecular biology

authors： Ke J,Chen RZ,Ban T,Zhou XE,Gu X,Tan MH,Chen C,Kang Y,Brunzelle JS,Zhu JK,Melcher K,Xu HE

更新日期：2013-12-01 00:00:00

abstract：:Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused re...

journal_title：Nature structural & molecular biology

authors： Campagne S,Marsh ME,Capitani G,Vorholt JA,Allain FH

更新日期：2014-03-01 00:00:00

abstract：:Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that a...

journal_title：Nature structural & molecular biology

authors： Takeshita D,Tomita K

更新日期：2012-01-15 00:00:00

abstract：:Trimethylation of Lys36 in histone H3 (H3K36me3) coordinates events associated with the elongation phase of transcription and is also emerging as an important epigenetic regulator of cell growth and differentiation. We have identified the PWWP domain of bromo and plant homeodomain (PHD) finger-containing protein 1 (BR...

journal_title：Nature structural & molecular biology

authors： Vezzoli A,Bonadies N,Allen MD,Freund SM,Santiveri CM,Kvinlaug BT,Huntly BJ,Göttgens B,Bycroft M

更新日期：2010-05-01 00:00:00

abstract：:The GTPase dynamin is essential for numerous vesiculation events including clathrin-mediated endocytosis. Upon GTP hydrolysis, dynamin constricts a lipid bilayer. Previously, a three-dimensional structure of mutant dynamin in the constricted state was determined by helical reconstruction methods. We solved the noncons...

journal_title：Nature structural & molecular biology

authors： Chen YJ,Zhang P,Egelman EH,Hinshaw JE

更新日期：2004-06-01 00:00:00