Abstract：
:The function of human XPA protein, a key subunit of the nucleotide excision repair pathway, has been examined with site-directed substitutions in its putative DNA-binding cleft. After screening for repair activity in a host-cell reactivation assay, we analyzed mutants by comparing their affinities for different substrate architectures, including DNA junctions that provide a surrogate for distorted reaction intermediates, and by testing their ability to recruit the downstream endonuclease partner. Normal repair proficiency was retained when XPA mutations abolished only the simple interaction with linear DNA molecules. By contrast, results from a K141E K179E double mutant revealed that excision is crucially dependent on the assembly of XPA protein with a sharp bending angle in the DNA substrate. These findings show how an increased deformability of damaged sites, leading to helical kinks recognized by XPA, contributes to target selectivity in DNA repair.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Camenisch U,Dip R,Schumacher SB,Schuler B,Naegeli Hdoi
10.1038/nsmb1061keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
278-84issue
3eissn
1545-9993issn
1545-9985pii
nsmb1061journal_volume
13pub_type
杂志文章abstract：:We investigated co-transcriptional recruitment of pre-mRNA processing factors to human genes. Capping factors associate with paused RNA polymerase II (pol II) at the 5' ends of quiescent genes. They also track throughout actively transcribed genes and accumulate with paused polymerase in the 3' flanking region. The 3'...
journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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abstract：:The noncoding RNA Xist recruits silencing factors to the inactive X chromosome (Xi) and facilitates re-organization of Xi structure. Here, we examine the mouse epigenomic landscape of Xi and assess how Xist alters chromatin accessibility. Xist deletion triggers a gain of accessibility of select chromatin regions that ...
journal_title：Nature structural & molecular biology
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
doi：10.1038/nsmb1286
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journal_title：Nature structural & molecular biology
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2020-08-01 00:00:00
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journal_title：Nature structural & molecular biology
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pub_type： 杂志文章
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abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature structural & molecular biology
pub_type： 已发布勘误
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更新日期：2020-09-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 评论,社论
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journal_title：Nature structural & molecular biology
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journal_title：Nature structural & molecular biology
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doi：10.1038/nsmb749
更新日期：2004-04-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
doi：10.1038/nsmb.1571
更新日期：2009-04-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
doi：10.1038/nsmb1128
更新日期：2006-08-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
doi：10.1038/nsmb.2147
更新日期：2011-10-30 00:00:00
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journal_title：Nature structural & molecular biology
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2008-08-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2004-09-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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更新日期：2008-08-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2004-12-01 00:00:00
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pub_type： 杂志文章
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章,评审
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更新日期：2015-11-01 00:00:00
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journal_title：Nature structural & molecular biology
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
