Abstract:
:The endosomal sorting complex required for transport (ESCRT) machinery, including ESCRT-III, localizes to the midbody and participates in the membrane-abscission step of cytokinesis. The ESCRT-III protein charged multivesicular body protein 1B (CHMP1B) is required for recruitment of the MIT domain-containing protein spastin, a microtubule-severing enzyme, to the midbody. The 2.5-A structure of the C-terminal tail of CHMP1B with the MIT domain of spastin reveals a specific, high-affinity complex involving a noncanonical binding site between the first and third helices of the MIT domain. The structural interface is twice as large as that of the MIT domain of the VPS4-CHMP complex, consistent with the high affinity of the interaction. A series of unique hydrogen-bonding interactions and close packing of small side chains discriminate against the other ten human ESCRT-III subunits. Point mutants in the CHMP1B binding site of spastin block recruitment of spastin to the midbody and impair cytokinesis.
journal_name
Nat Struct Mol Bioljournal_title
Nature structural & molecular biologyauthors
Yang D,Rismanchi N,Renvoisé B,Lippincott-Schwartz J,Blackstone C,Hurley JHdoi
10.1038/nsmb.1512subject
Has Abstractpub_date
2008-12-01 00:00:00pages
1278-86issue
12eissn
1545-9993issn
1545-9985pii
nsmb.1512journal_volume
15pub_type
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